N-Nornuciferine Promotes Human Non-Small Cell Lung Cancer A549 Cells Apoptosis via miR-361-3p/TRAF2/JNK Axis
Background: N-Nornuciferine (N-NF) is an aporphine alkaloid in lotus leaf with a good resource for obtaining the biologically active substances with antioxidant properties. The purpose of this study was to explore the effect of N-NF on cell viability and apoptosis in A549 cells in vitro culture and...
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Shiraz University of Medical Sciences
2025-04-01
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| Series: | Middle East Journal of Cancer |
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| Online Access: | https://mejc.sums.ac.ir/article_50310_b70d4f3d6908135d40efd23be7245430.pdf |
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| author | Chengxiang Wang Lin Bai |
| author_facet | Chengxiang Wang Lin Bai |
| author_sort | Chengxiang Wang |
| collection | DOAJ |
| description | Background: N-Nornuciferine (N-NF) is an aporphine alkaloid in lotus leaf with a good resource for obtaining the biologically active substances with antioxidant properties. The purpose of this study was to explore the effect of N-NF on cell viability and apoptosis in A549 cells in vitro culture and to explore its mechanism of action.Method: In this experimental study, we cultured A549 cells in vitro and added different concentrations of N-NF for intervention to explore cell apoptosis and its mechanism. Cell viability was detected by CCK-8, and cell apoptosis level was detected by flow cytometry. The mechanism of action of N-NF was explored by regulating the expression of miR-361-3p. The data were analyzed using the one-way analysis of variance (ANOVA) technique in SPSS 22.0 software, with statistical significance set at a level of P < 0.05.Results: The study results showed that N-NF could significantly inhibit the viability of A549 cells and promote apoptosis. Using dual-luciferase reporter gene and molecular biology detection experiments, it was found that N-NF inhibits the viability of A549 cells through inhibiting the expression of miR-361-3p and promoting the TRAF2/JNK pathway.Conclusion: This study provides a basis for the development of N-NF as an anticancer drug and explains its mechanism of promoting apoptosis of A549 cells. |
| format | Article |
| id | doaj-art-e74aeb8fd97e4fdd8a9f964151df8d7d |
| institution | OA Journals |
| issn | 2008-6709 2008-6687 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Middle East Journal of Cancer |
| spelling | doaj-art-e74aeb8fd97e4fdd8a9f964151df8d7d2025-08-20T02:15:50ZengShiraz University of Medical SciencesMiddle East Journal of Cancer2008-67092008-66872025-04-0116213914910.30476/mejc.2024.101824.204650310N-Nornuciferine Promotes Human Non-Small Cell Lung Cancer A549 Cells Apoptosis via miR-361-3p/TRAF2/JNK AxisChengxiang Wang0Lin Bai1School of Basic Medicine, Guangxi University of Traditional Chinese Medicine, Nanning, ChinaSchool of Basic Medicine, Guangxi University of Traditional Chinese Medicine, Nanning, ChinaBackground: N-Nornuciferine (N-NF) is an aporphine alkaloid in lotus leaf with a good resource for obtaining the biologically active substances with antioxidant properties. The purpose of this study was to explore the effect of N-NF on cell viability and apoptosis in A549 cells in vitro culture and to explore its mechanism of action.Method: In this experimental study, we cultured A549 cells in vitro and added different concentrations of N-NF for intervention to explore cell apoptosis and its mechanism. Cell viability was detected by CCK-8, and cell apoptosis level was detected by flow cytometry. The mechanism of action of N-NF was explored by regulating the expression of miR-361-3p. The data were analyzed using the one-way analysis of variance (ANOVA) technique in SPSS 22.0 software, with statistical significance set at a level of P < 0.05.Results: The study results showed that N-NF could significantly inhibit the viability of A549 cells and promote apoptosis. Using dual-luciferase reporter gene and molecular biology detection experiments, it was found that N-NF inhibits the viability of A549 cells through inhibiting the expression of miR-361-3p and promoting the TRAF2/JNK pathway.Conclusion: This study provides a basis for the development of N-NF as an anticancer drug and explains its mechanism of promoting apoptosis of A549 cells.https://mejc.sums.ac.ir/article_50310_b70d4f3d6908135d40efd23be7245430.pdfmicrornasapoptosiscarcinomanon-small-cell lung |
| spellingShingle | Chengxiang Wang Lin Bai N-Nornuciferine Promotes Human Non-Small Cell Lung Cancer A549 Cells Apoptosis via miR-361-3p/TRAF2/JNK Axis Middle East Journal of Cancer micrornas apoptosis carcinoma non-small-cell lung |
| title | N-Nornuciferine Promotes Human Non-Small Cell Lung Cancer A549 Cells Apoptosis via miR-361-3p/TRAF2/JNK Axis |
| title_full | N-Nornuciferine Promotes Human Non-Small Cell Lung Cancer A549 Cells Apoptosis via miR-361-3p/TRAF2/JNK Axis |
| title_fullStr | N-Nornuciferine Promotes Human Non-Small Cell Lung Cancer A549 Cells Apoptosis via miR-361-3p/TRAF2/JNK Axis |
| title_full_unstemmed | N-Nornuciferine Promotes Human Non-Small Cell Lung Cancer A549 Cells Apoptosis via miR-361-3p/TRAF2/JNK Axis |
| title_short | N-Nornuciferine Promotes Human Non-Small Cell Lung Cancer A549 Cells Apoptosis via miR-361-3p/TRAF2/JNK Axis |
| title_sort | n nornuciferine promotes human non small cell lung cancer a549 cells apoptosis via mir 361 3p traf2 jnk axis |
| topic | micrornas apoptosis carcinoma non-small-cell lung |
| url | https://mejc.sums.ac.ir/article_50310_b70d4f3d6908135d40efd23be7245430.pdf |
| work_keys_str_mv | AT chengxiangwang nnornuciferinepromoteshumannonsmallcelllungcancera549cellsapoptosisviamir3613ptraf2jnkaxis AT linbai nnornuciferinepromoteshumannonsmallcelllungcancera549cellsapoptosisviamir3613ptraf2jnkaxis |