Antibiotic residues correlate with antibiotic resistance of Salmonella typhimurium isolated from edible chicken meat

Abstract Irrational application and overuse of antibiotics in poultry production is associated with antibiotic residues in meat tissues which lead to the development of antibiotic resistance in pathogenic bacteria such as Salmonella. This study investigated the co-existence of multi-drug resistant S...

Full description

Saved in:
Bibliographic Details
Main Authors: Hala R. Ali, Esraa G. Hefny, Naglaa F. Koraney, Samah F. Ali, Mohamed I. AbdAllah, Mai A. Fadel, Sara M. Elnomrosy, Momataz A. Shahein
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Scientific Reports
Subjects:
Online Access:https://doi.org/10.1038/s41598-025-98189-4
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850284508811624448
author Hala R. Ali
Esraa G. Hefny
Naglaa F. Koraney
Samah F. Ali
Mohamed I. AbdAllah
Mai A. Fadel
Sara M. Elnomrosy
Momataz A. Shahein
author_facet Hala R. Ali
Esraa G. Hefny
Naglaa F. Koraney
Samah F. Ali
Mohamed I. AbdAllah
Mai A. Fadel
Sara M. Elnomrosy
Momataz A. Shahein
author_sort Hala R. Ali
collection DOAJ
description Abstract Irrational application and overuse of antibiotics in poultry production is associated with antibiotic residues in meat tissues which lead to the development of antibiotic resistance in pathogenic bacteria such as Salmonella. This study investigated the co-existence of multi-drug resistant Salmonella typhimurium (S. typhimurium) and residual traces of antibiotics in chilled chicken meat collected from Fayoum province in Egypt. Our data reported that 22.2% of chicken meat and liver were contaminated with S. typhimurium with 100% being drug resistant to a wide range of antimicrobials including tylosin, chloramphenicol, and oxytetracycline (OTC). The residual analysis of chicken meat samples revealed that they contained residues of 3antibacterial drugs including tylosin, chloramphenicol, OTC and its metabolite (4-epi-OTC). The statistical analysis of obtained data also revealed a relationship between S. typhimurium resistance and the presence of antibiotic residues in chicken samples, as isolates derived from samples with residual drug content higher than maximum residue levels (MRL) were found to be resistant to the same antibiotic. This confirms the importance of integrating sustainable practices in the veterinary field with a strict commitment to monitoring the bacterial content and residual antibiotics in foods of animal origin before marketing them, to protect the effectiveness of antibiotics for future generations.
format Article
id doaj-art-e73e2ce1ea274658bde6e77756adfd21
institution OA Journals
issn 2045-2322
language English
publishDate 2025-04-01
publisher Nature Portfolio
record_format Article
series Scientific Reports
spelling doaj-art-e73e2ce1ea274658bde6e77756adfd212025-08-20T01:47:33ZengNature PortfolioScientific Reports2045-23222025-04-0115111210.1038/s41598-025-98189-4Antibiotic residues correlate with antibiotic resistance of Salmonella typhimurium isolated from edible chicken meatHala R. Ali0Esraa G. Hefny1Naglaa F. Koraney2Samah F. Ali3Mohamed I. AbdAllah4Mai A. Fadel5Sara M. Elnomrosy6Momataz A. Shahein7Bacteriology Department, Animal Health Research Institute (AHRI), Agriculture Research Centre (ARC)Food Hygiene Department, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC)Bacteriology Department, Animal Health Research Institute (AHRI), Agriculture Research Centre (ARC)Bacteriology Department, Animal Health Research Institute (AHRI), Agriculture Research Centre (ARC)Genome Research Unit, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC)Pharmacology and Pyrogen Unit, Department of Biochemistry, Toxicology and Feed Deficiency, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC)Genome Research Unit, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC)Department of Virology Research, Animal Health Research Institute, Agriculture Research Center (ARC)Abstract Irrational application and overuse of antibiotics in poultry production is associated with antibiotic residues in meat tissues which lead to the development of antibiotic resistance in pathogenic bacteria such as Salmonella. This study investigated the co-existence of multi-drug resistant Salmonella typhimurium (S. typhimurium) and residual traces of antibiotics in chilled chicken meat collected from Fayoum province in Egypt. Our data reported that 22.2% of chicken meat and liver were contaminated with S. typhimurium with 100% being drug resistant to a wide range of antimicrobials including tylosin, chloramphenicol, and oxytetracycline (OTC). The residual analysis of chicken meat samples revealed that they contained residues of 3antibacterial drugs including tylosin, chloramphenicol, OTC and its metabolite (4-epi-OTC). The statistical analysis of obtained data also revealed a relationship between S. typhimurium resistance and the presence of antibiotic residues in chicken samples, as isolates derived from samples with residual drug content higher than maximum residue levels (MRL) were found to be resistant to the same antibiotic. This confirms the importance of integrating sustainable practices in the veterinary field with a strict commitment to monitoring the bacterial content and residual antibiotics in foods of animal origin before marketing them, to protect the effectiveness of antibiotics for future generations.https://doi.org/10.1038/s41598-025-98189-4SalmonellaS. typhimuriumAntimicrobialsAntibiotic residues
spellingShingle Hala R. Ali
Esraa G. Hefny
Naglaa F. Koraney
Samah F. Ali
Mohamed I. AbdAllah
Mai A. Fadel
Sara M. Elnomrosy
Momataz A. Shahein
Antibiotic residues correlate with antibiotic resistance of Salmonella typhimurium isolated from edible chicken meat
Scientific Reports
Salmonella
S. typhimurium
Antimicrobials
Antibiotic residues
title Antibiotic residues correlate with antibiotic resistance of Salmonella typhimurium isolated from edible chicken meat
title_full Antibiotic residues correlate with antibiotic resistance of Salmonella typhimurium isolated from edible chicken meat
title_fullStr Antibiotic residues correlate with antibiotic resistance of Salmonella typhimurium isolated from edible chicken meat
title_full_unstemmed Antibiotic residues correlate with antibiotic resistance of Salmonella typhimurium isolated from edible chicken meat
title_short Antibiotic residues correlate with antibiotic resistance of Salmonella typhimurium isolated from edible chicken meat
title_sort antibiotic residues correlate with antibiotic resistance of salmonella typhimurium isolated from edible chicken meat
topic Salmonella
S. typhimurium
Antimicrobials
Antibiotic residues
url https://doi.org/10.1038/s41598-025-98189-4
work_keys_str_mv AT halarali antibioticresiduescorrelatewithantibioticresistanceofsalmonellatyphimuriumisolatedfromediblechickenmeat
AT esraaghefny antibioticresiduescorrelatewithantibioticresistanceofsalmonellatyphimuriumisolatedfromediblechickenmeat
AT naglaafkoraney antibioticresiduescorrelatewithantibioticresistanceofsalmonellatyphimuriumisolatedfromediblechickenmeat
AT samahfali antibioticresiduescorrelatewithantibioticresistanceofsalmonellatyphimuriumisolatedfromediblechickenmeat
AT mohamediabdallah antibioticresiduescorrelatewithantibioticresistanceofsalmonellatyphimuriumisolatedfromediblechickenmeat
AT maiafadel antibioticresiduescorrelatewithantibioticresistanceofsalmonellatyphimuriumisolatedfromediblechickenmeat
AT saramelnomrosy antibioticresiduescorrelatewithantibioticresistanceofsalmonellatyphimuriumisolatedfromediblechickenmeat
AT momatazashahein antibioticresiduescorrelatewithantibioticresistanceofsalmonellatyphimuriumisolatedfromediblechickenmeat