Chemical Composition, Antioxidant Potential, and Antimicrobial Activity of Novel Antiseptic Lotion from the Leaves of <i>Betula pendula</i> Roth
Plants of the genus <i>Betula pendula</i> (Betulaceae) have been used as antiseptics in the treatment of skin diseases. While the bark of this plant has so far been used as a medicinal raw material, our research has revealed that the leaves are an extremely valuable source of health-prom...
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| author | Łukasz Kucharski Edyta Kucharska Anna Muzykiewicz-Szymańska Anna Nowak Robert Pełech |
| author_facet | Łukasz Kucharski Edyta Kucharska Anna Muzykiewicz-Szymańska Anna Nowak Robert Pełech |
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| description | Plants of the genus <i>Betula pendula</i> (Betulaceae) have been used as antiseptics in the treatment of skin diseases. While the bark of this plant has so far been used as a medicinal raw material, our research has revealed that the leaves are an extremely valuable source of health-promoting bioactive compounds, especially polyphenolic compounds. The antioxidant activity of a novel lotion from the <i>Betula pendula</i> leaf and its phenolic content was determined using the DPPH and FRAP assays and the Folin–Ciocalteu method, and a qualitative analysis was conducted using the GC-MS method. The antiseptic preparation was also subjected to HPLC analysis for an assessment of the phenolic acids content (gallic, 3,4-dihydroxybenzoic, 2,5-dihydroxybenzoic, chlorogenic, and 3-hydroxybenzoic acids). The shake flask method was used to determine the partition coefficient of the lotion to assess its lipophilicity. A new ethanol-reduced antiseptic lotion containing antimicrobial ingredients was evaluated using standard killing methods (EN 13697:2019 and EN 13697:2015) at 60 ± 10 s and 300 ± 10 s against six microorganisms. The aim of these tests was to determine whether the formulation exhibited performance like a traditional ethanol. In addition, hand disinfection tests were also carried out using the preparation obtained from <i>B. pendula</i> leaves in accordance with EN 1500:2013, using <i>Escherichia coli</i> strain K12 NCTC 10538. According to the results, high polyphenol contents (52 ± 2 mg GAE/g dry raw material) and other antioxidant and biologically active substances identified by GC-MS (phytol, alpha-tocospiro B, sitosterol, or dilauryl thiodipropionate) can be thought of as the parameters responsible for the effective activity of the preparation derived from birch leaves. Gallic acid and 3,4-dihydroxybenzoic acid had the greatest concentrations among the phenolic acids examined. The mean 60 s kill time was >99.9 for the new antiseptic against <i>Staphylococcus aureus</i> ATCC 6538 (at a concentration of 30 g/100 mL) and <i>Pseudomonas aeruginosa</i> ATCC 15442 (at a concentration of 40 g/100 mL), while ethanol showed the same reduction for these microorganisms at a concentration of 80 g/100 mL. |
| format | Article |
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| publishDate | 2025-03-01 |
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| spelling | doaj-art-e73cfaed821e4175a9db36d1cc93ca4e2025-08-20T03:06:24ZengMDPI AGApplied Sciences2076-34172025-03-01157365810.3390/app15073658Chemical Composition, Antioxidant Potential, and Antimicrobial Activity of Novel Antiseptic Lotion from the Leaves of <i>Betula pendula</i> RothŁukasz Kucharski0Edyta Kucharska1Anna Muzykiewicz-Szymańska2Anna Nowak3Robert Pełech4Department of Cosmetic and Pharmaceutical Chemistry, Pomeranian Medical University in Szczecin, PL-70111 Szczecin, PolandDepartment of Organic Chemical Technology and Polymeric Materials, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology, Szczecin, PL-70322 Szczecin, PolandDepartment of Cosmetic and Pharmaceutical Chemistry, Pomeranian Medical University in Szczecin, PL-70111 Szczecin, PolandDepartment of Cosmetic and Pharmaceutical Chemistry, Pomeranian Medical University in Szczecin, PL-70111 Szczecin, PolandDepartment of Organic Chemical Technology and Polymeric Materials, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology, Szczecin, PL-70322 Szczecin, PolandPlants of the genus <i>Betula pendula</i> (Betulaceae) have been used as antiseptics in the treatment of skin diseases. While the bark of this plant has so far been used as a medicinal raw material, our research has revealed that the leaves are an extremely valuable source of health-promoting bioactive compounds, especially polyphenolic compounds. The antioxidant activity of a novel lotion from the <i>Betula pendula</i> leaf and its phenolic content was determined using the DPPH and FRAP assays and the Folin–Ciocalteu method, and a qualitative analysis was conducted using the GC-MS method. The antiseptic preparation was also subjected to HPLC analysis for an assessment of the phenolic acids content (gallic, 3,4-dihydroxybenzoic, 2,5-dihydroxybenzoic, chlorogenic, and 3-hydroxybenzoic acids). The shake flask method was used to determine the partition coefficient of the lotion to assess its lipophilicity. A new ethanol-reduced antiseptic lotion containing antimicrobial ingredients was evaluated using standard killing methods (EN 13697:2019 and EN 13697:2015) at 60 ± 10 s and 300 ± 10 s against six microorganisms. The aim of these tests was to determine whether the formulation exhibited performance like a traditional ethanol. In addition, hand disinfection tests were also carried out using the preparation obtained from <i>B. pendula</i> leaves in accordance with EN 1500:2013, using <i>Escherichia coli</i> strain K12 NCTC 10538. According to the results, high polyphenol contents (52 ± 2 mg GAE/g dry raw material) and other antioxidant and biologically active substances identified by GC-MS (phytol, alpha-tocospiro B, sitosterol, or dilauryl thiodipropionate) can be thought of as the parameters responsible for the effective activity of the preparation derived from birch leaves. Gallic acid and 3,4-dihydroxybenzoic acid had the greatest concentrations among the phenolic acids examined. The mean 60 s kill time was >99.9 for the new antiseptic against <i>Staphylococcus aureus</i> ATCC 6538 (at a concentration of 30 g/100 mL) and <i>Pseudomonas aeruginosa</i> ATCC 15442 (at a concentration of 40 g/100 mL), while ethanol showed the same reduction for these microorganisms at a concentration of 80 g/100 mL.https://www.mdpi.com/2076-3417/15/7/3658leaves of <i>Betula pendula</i> Rothbiologically active preparationslog Pbioactive compoundsantioxidative potentialantimicrobial activity |
| spellingShingle | Łukasz Kucharski Edyta Kucharska Anna Muzykiewicz-Szymańska Anna Nowak Robert Pełech Chemical Composition, Antioxidant Potential, and Antimicrobial Activity of Novel Antiseptic Lotion from the Leaves of <i>Betula pendula</i> Roth Applied Sciences leaves of <i>Betula pendula</i> Roth biologically active preparations log P bioactive compounds antioxidative potential antimicrobial activity |
| title | Chemical Composition, Antioxidant Potential, and Antimicrobial Activity of Novel Antiseptic Lotion from the Leaves of <i>Betula pendula</i> Roth |
| title_full | Chemical Composition, Antioxidant Potential, and Antimicrobial Activity of Novel Antiseptic Lotion from the Leaves of <i>Betula pendula</i> Roth |
| title_fullStr | Chemical Composition, Antioxidant Potential, and Antimicrobial Activity of Novel Antiseptic Lotion from the Leaves of <i>Betula pendula</i> Roth |
| title_full_unstemmed | Chemical Composition, Antioxidant Potential, and Antimicrobial Activity of Novel Antiseptic Lotion from the Leaves of <i>Betula pendula</i> Roth |
| title_short | Chemical Composition, Antioxidant Potential, and Antimicrobial Activity of Novel Antiseptic Lotion from the Leaves of <i>Betula pendula</i> Roth |
| title_sort | chemical composition antioxidant potential and antimicrobial activity of novel antiseptic lotion from the leaves of i betula pendula i roth |
| topic | leaves of <i>Betula pendula</i> Roth biologically active preparations log P bioactive compounds antioxidative potential antimicrobial activity |
| url | https://www.mdpi.com/2076-3417/15/7/3658 |
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