Spectrofluorimetric determination of gemifloxacin mesylate and linezolid in pharmaceutical formulations: Application of quinone-based fluorophores and enhanced native fluorescence
Quinone-based fluorophores and enhanced native fluorescence techniques were applied for a fast quantitative analysis of gemifloxacin mesylate (GEM) and linezolid (LIN) in pharmaceutical formulations. For this purpose, three sensitive, accurate and precise spectrofluorimetric methods were developed....
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Sciendo
2014-03-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | http://www.degruyter.com/view/j/acph.2014.64.issue-1/acph-2014-0005/acph-2014-0005.xml?format=INT |
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| author | Moussa Bahia Abbas Mahrouse Marianne Alphonse Hassan Mahmoud Ali Fawzy Michael Gamal |
| author_facet | Moussa Bahia Abbas Mahrouse Marianne Alphonse Hassan Mahmoud Ali Fawzy Michael Gamal |
| author_sort | Moussa Bahia Abbas |
| collection | DOAJ |
| description | Quinone-based fluorophores and enhanced native fluorescence techniques were applied for a fast quantitative analysis of gemifloxacin mesylate (GEM) and linezolid (LIN) in pharmaceutical formulations. For this purpose, three sensitive, accurate and precise spectrofluorimetric methods were developed. GEM, as an n-electron donor, reacts with 7,7,8,8-tetracyanoquinodimethane (method A) and 2,5-dichloro-3,6-dihydroxy-p-benzoquinone (method B) as п-electron acceptors, forming charge transfer complexes that exhibit high fluorescence intensity at 441 and 390 nm upon excitation at 260 and 339 nm, respectively. Method C depends on measurement of enhanced native fluorescence of LIN in phosphate buffer (pH 5) at 380 nm upon excitation at 260 nm. Experimental factors affecting fluorescence intensity were optimized. Linearity was obtained over concentration ranges 50-500, 10-60 and 20-400 ng mL-1 for methods A, B and C, respectively. The developed methods were validated and successfully applied for determination of the cited drugs in tablets. |
| format | Article |
| id | doaj-art-e72b8ef9103941c7a5e55040fd08a180 |
| institution | Kabale University |
| issn | 1330-0075 1846-9558 |
| language | English |
| publishDate | 2014-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-e72b8ef9103941c7a5e55040fd08a1802025-02-02T13:20:29ZengSciendoActa Pharmaceutica1330-00751846-95582014-03-01641152810.2478/acph-2014-0005acph-2014-0005Spectrofluorimetric determination of gemifloxacin mesylate and linezolid in pharmaceutical formulations: Application of quinone-based fluorophores and enhanced native fluorescenceMoussa Bahia Abbas0Mahrouse Marianne Alphonse1Hassan Mahmoud Ali2Fawzy Michael Gamal3Pharmaceutical Chemistry Department Faculty of Pharmacy, Cairo University Kasr El-Aini St., Cairo 11562, EgyptPharmaceutical Chemistry Department Faculty of Pharmacy, Cairo University Kasr El-Aini St., Cairo 11562, EgyptPharmaceutical Medicinal Chemistry Department, Faculty of Pharmacy Egyptian Russian University, Badr City Cairo, 11829, EgyptPharmaceutical Medicinal Chemistry Department, Faculty of Pharmacy Egyptian Russian University, Badr City Cairo, 11829, EgyptQuinone-based fluorophores and enhanced native fluorescence techniques were applied for a fast quantitative analysis of gemifloxacin mesylate (GEM) and linezolid (LIN) in pharmaceutical formulations. For this purpose, three sensitive, accurate and precise spectrofluorimetric methods were developed. GEM, as an n-electron donor, reacts with 7,7,8,8-tetracyanoquinodimethane (method A) and 2,5-dichloro-3,6-dihydroxy-p-benzoquinone (method B) as п-electron acceptors, forming charge transfer complexes that exhibit high fluorescence intensity at 441 and 390 nm upon excitation at 260 and 339 nm, respectively. Method C depends on measurement of enhanced native fluorescence of LIN in phosphate buffer (pH 5) at 380 nm upon excitation at 260 nm. Experimental factors affecting fluorescence intensity were optimized. Linearity was obtained over concentration ranges 50-500, 10-60 and 20-400 ng mL-1 for methods A, B and C, respectively. The developed methods were validated and successfully applied for determination of the cited drugs in tablets.http://www.degruyter.com/view/j/acph.2014.64.issue-1/acph-2014-0005/acph-2014-0005.xml?format=INTgemifloxacin mesylatelinezolid7,7,8,8-tetracyanoquinodimethane2,5-dichloro-3,6-dihydroxy-p-ben- -zoquinonefluorimetrycharge transfer complex |
| spellingShingle | Moussa Bahia Abbas Mahrouse Marianne Alphonse Hassan Mahmoud Ali Fawzy Michael Gamal Spectrofluorimetric determination of gemifloxacin mesylate and linezolid in pharmaceutical formulations: Application of quinone-based fluorophores and enhanced native fluorescence Acta Pharmaceutica gemifloxacin mesylate linezolid 7,7,8,8-tetracyanoquinodimethane 2,5-dichloro-3,6-dihydroxy-p-ben- -zoquinone fluorimetry charge transfer complex |
| title | Spectrofluorimetric determination of gemifloxacin mesylate and linezolid in pharmaceutical formulations: Application of quinone-based fluorophores and enhanced native fluorescence |
| title_full | Spectrofluorimetric determination of gemifloxacin mesylate and linezolid in pharmaceutical formulations: Application of quinone-based fluorophores and enhanced native fluorescence |
| title_fullStr | Spectrofluorimetric determination of gemifloxacin mesylate and linezolid in pharmaceutical formulations: Application of quinone-based fluorophores and enhanced native fluorescence |
| title_full_unstemmed | Spectrofluorimetric determination of gemifloxacin mesylate and linezolid in pharmaceutical formulations: Application of quinone-based fluorophores and enhanced native fluorescence |
| title_short | Spectrofluorimetric determination of gemifloxacin mesylate and linezolid in pharmaceutical formulations: Application of quinone-based fluorophores and enhanced native fluorescence |
| title_sort | spectrofluorimetric determination of gemifloxacin mesylate and linezolid in pharmaceutical formulations application of quinone based fluorophores and enhanced native fluorescence |
| topic | gemifloxacin mesylate linezolid 7,7,8,8-tetracyanoquinodimethane 2,5-dichloro-3,6-dihydroxy-p-ben- -zoquinone fluorimetry charge transfer complex |
| url | http://www.degruyter.com/view/j/acph.2014.64.issue-1/acph-2014-0005/acph-2014-0005.xml?format=INT |
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