Methylenetetrahydrofolate reductase gene polymorphisms, lipid profiles, and basic renal functional markers as risk for myocardial infarction: A case-control study and haplotype analysis
Myocardial infarction (MI) is a serious cardiovascular disease and the primary cause of mortality, with a complex etiopathology. Identifying the genetic basis of myocardial infarction (MI) is essential for developing personalized medical treatments. This study examined the possible association betwe...
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University of Belgrade, University of Novi Sad
2024-01-01
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| Series: | Archives of Biological Sciences |
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| Online Access: | https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400035P.pdf |
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| author | Perović Svetlana Vujović Slavica Kapur-Pojskić Lejla Garai Nemanja Šćepanović Anđelka |
| author_facet | Perović Svetlana Vujović Slavica Kapur-Pojskić Lejla Garai Nemanja Šćepanović Anđelka |
| author_sort | Perović Svetlana |
| collection | DOAJ |
| description | Myocardial infarction (MI) is a serious cardiovascular disease and the primary cause of mortality, with a complex etiopathology. Identifying the genetic basis of myocardial infarction (MI) is essential for developing personalized medical treatments. This study examined the possible association between polymorphisms in the methylenetetrahydrofolate reductase (MTHFR) gene and MI. In the study, 120 patients with MI and 120 age-and-sex-matched controls were genotyped for C677T and A1298C MTHFR polymorphisms by the allele-specific or amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). In the case of the C677T polymorphism, the T/T and C/T genotypes were associated with a significantly increased risk of MI under the dominant genetic model (odds ratio (OR)=2.060; P=0.006). Although there was no significant association between the A1298C variant and MI, this polymorphism was linked to a higher level of creatinine in MI patients (P<0.002). A similar association was observed for the C677T polymorphism (P=0.003). An A-T haplotype represented an increased risk for MI (OR=1.630; P=0.014), whereas the A-C haplotype had a protective role (R=0.517; P=0.002). These findings indicate that C677T MTHFR polymorphism is strongly associated with and increased risk of MI, making it a potential genetic risk factor and a possible predictor of MI. |
| format | Article |
| id | doaj-art-e729a8bb5cfc4c14a0dd9fb65e8fa983 |
| institution | Kabale University |
| issn | 0354-4664 1821-4339 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj-art-e729a8bb5cfc4c14a0dd9fb65e8fa9832025-01-30T06:38:39ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392024-01-0176445546410.2298/ABS240801035P0354-46642400035PMethylenetetrahydrofolate reductase gene polymorphisms, lipid profiles, and basic renal functional markers as risk for myocardial infarction: A case-control study and haplotype analysisPerović Svetlana0https://orcid.org/0009-0009-3973-2907Vujović Slavica1https://orcid.org/0000-0002-4024-9009Kapur-Pojskić Lejla2https://orcid.org/0000-0003-2260-318XGarai Nemanja3https://orcid.org/0000-0002-8194-6329Šćepanović Anđelka4https://orcid.org/0000-0002-2094-6245Department of Biology, Faculty of Natural Sciences and Mathematics, University of Montenegro, Podgorica, MontenegroDepartment of Biology, Faculty of Natural Sciences and Mathematics, University of Montenegro, Podgorica, MontenegroInstitute of Genetic Engineering and Biotechnology, University of Sarajevo, Sarajevo, Bosnia and HerzegovinaCenter for Human Molecular Genetics, Faculty of Biology, University of Belgrade, Belgrade, SerbiaDepartment of Biology, Faculty of Natural Sciences and Mathematics, University of Montenegro, Podgorica, MontenegroMyocardial infarction (MI) is a serious cardiovascular disease and the primary cause of mortality, with a complex etiopathology. Identifying the genetic basis of myocardial infarction (MI) is essential for developing personalized medical treatments. This study examined the possible association between polymorphisms in the methylenetetrahydrofolate reductase (MTHFR) gene and MI. In the study, 120 patients with MI and 120 age-and-sex-matched controls were genotyped for C677T and A1298C MTHFR polymorphisms by the allele-specific or amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). In the case of the C677T polymorphism, the T/T and C/T genotypes were associated with a significantly increased risk of MI under the dominant genetic model (odds ratio (OR)=2.060; P=0.006). Although there was no significant association between the A1298C variant and MI, this polymorphism was linked to a higher level of creatinine in MI patients (P<0.002). A similar association was observed for the C677T polymorphism (P=0.003). An A-T haplotype represented an increased risk for MI (OR=1.630; P=0.014), whereas the A-C haplotype had a protective role (R=0.517; P=0.002). These findings indicate that C677T MTHFR polymorphism is strongly associated with and increased risk of MI, making it a potential genetic risk factor and a possible predictor of MI.https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400035P.pdfmyocardial infarctionmethylenetetrahydrofolate reductase gene polymorphismsmyocardial infarction risk factorshaplotype analysisrenal functional markers |
| spellingShingle | Perović Svetlana Vujović Slavica Kapur-Pojskić Lejla Garai Nemanja Šćepanović Anđelka Methylenetetrahydrofolate reductase gene polymorphisms, lipid profiles, and basic renal functional markers as risk for myocardial infarction: A case-control study and haplotype analysis Archives of Biological Sciences myocardial infarction methylenetetrahydrofolate reductase gene polymorphisms myocardial infarction risk factors haplotype analysis renal functional markers |
| title | Methylenetetrahydrofolate reductase gene polymorphisms, lipid profiles, and basic renal functional markers as risk for myocardial infarction: A case-control study and haplotype analysis |
| title_full | Methylenetetrahydrofolate reductase gene polymorphisms, lipid profiles, and basic renal functional markers as risk for myocardial infarction: A case-control study and haplotype analysis |
| title_fullStr | Methylenetetrahydrofolate reductase gene polymorphisms, lipid profiles, and basic renal functional markers as risk for myocardial infarction: A case-control study and haplotype analysis |
| title_full_unstemmed | Methylenetetrahydrofolate reductase gene polymorphisms, lipid profiles, and basic renal functional markers as risk for myocardial infarction: A case-control study and haplotype analysis |
| title_short | Methylenetetrahydrofolate reductase gene polymorphisms, lipid profiles, and basic renal functional markers as risk for myocardial infarction: A case-control study and haplotype analysis |
| title_sort | methylenetetrahydrofolate reductase gene polymorphisms lipid profiles and basic renal functional markers as risk for myocardial infarction a case control study and haplotype analysis |
| topic | myocardial infarction methylenetetrahydrofolate reductase gene polymorphisms myocardial infarction risk factors haplotype analysis renal functional markers |
| url | https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400035P.pdf |
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