Metagenomic investigation of viruses in green sea turtles (Chelonia mydas)
Green sea turtles are listed on the International Union for Conservation of Nature’s Red List of Threatened Species. Thus, conservation efforts, including investigation of factors affecting the health of green sea turtles, are critical. Viral communities play vital roles in maintaining animal health...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1492038/full |
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| author | Hongwei Li Yuan Chen Zhongrong Xia Daohua Zhuang Feng Cong Yue-Xiao Lian |
| author_facet | Hongwei Li Yuan Chen Zhongrong Xia Daohua Zhuang Feng Cong Yue-Xiao Lian |
| author_sort | Hongwei Li |
| collection | DOAJ |
| description | Green sea turtles are listed on the International Union for Conservation of Nature’s Red List of Threatened Species. Thus, conservation efforts, including investigation of factors affecting the health of green sea turtles, are critical. Viral communities play vital roles in maintaining animal health. In the present study, shotgun metagenomics was used for the first time to survey viruses in the feces of green sea turtles. Most viral contigs were DNA viruses that mainly belonged to Caudoviricetes, followed by Crassvirales. Additionally, most of the viral contigs were not assigned to any known family or genus, implying a large knowledge gap in the taxonomy of green sea turtle gut viruses. Host prediction showed that most viruses were connected to two phyla: Bacteroidetes and Firmicutes. Furthermore, KEGG enrichment analysis showed that the viral genes were mainly involved in phage-associated and metabolic pathways. Phylogenetic tree reconstruction of Caudovirales terminase large-subunit (TerL) protein showed that most of the sequences were phylogenetically distant. This study expands our understanding of the viral diversity in green sea turtles. In particular, analysis of the virome RNA fraction is exceedingly important for investigating intestinal viromes; therefore, future studies could use metatranscriptomics to study RNA viruses. |
| format | Article |
| id | doaj-art-e7296730d14b4c5b94e82b0b1dbd28a4 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-e7296730d14b4c5b94e82b0b1dbd28a42025-01-22T07:11:26ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011610.3389/fmicb.2025.14920381492038Metagenomic investigation of viruses in green sea turtles (Chelonia mydas)Hongwei Li0Yuan Chen1Zhongrong Xia2Daohua Zhuang3Feng Cong4Yue-Xiao Lian5School of Life Science, Huizhou University, Huizhou, ChinaSchool of Life Science, Huizhou University, Huizhou, ChinaGuangdong Huidong Sea Turtle National Nature Reserve Bureau, Sea Turtle Bay, Huizhou, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Life Sciences, Yunnan University, Kunming, ChinaGuangdong Laboratory Animal Monitoring Institute and Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou, ChinaGuangdong Laboratory Animal Monitoring Institute and Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou, ChinaGreen sea turtles are listed on the International Union for Conservation of Nature’s Red List of Threatened Species. Thus, conservation efforts, including investigation of factors affecting the health of green sea turtles, are critical. Viral communities play vital roles in maintaining animal health. In the present study, shotgun metagenomics was used for the first time to survey viruses in the feces of green sea turtles. Most viral contigs were DNA viruses that mainly belonged to Caudoviricetes, followed by Crassvirales. Additionally, most of the viral contigs were not assigned to any known family or genus, implying a large knowledge gap in the taxonomy of green sea turtle gut viruses. Host prediction showed that most viruses were connected to two phyla: Bacteroidetes and Firmicutes. Furthermore, KEGG enrichment analysis showed that the viral genes were mainly involved in phage-associated and metabolic pathways. Phylogenetic tree reconstruction of Caudovirales terminase large-subunit (TerL) protein showed that most of the sequences were phylogenetically distant. This study expands our understanding of the viral diversity in green sea turtles. In particular, analysis of the virome RNA fraction is exceedingly important for investigating intestinal viromes; therefore, future studies could use metatranscriptomics to study RNA viruses.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1492038/fullgreen sea turtleviromemetagenomicsfecesvirus |
| spellingShingle | Hongwei Li Yuan Chen Zhongrong Xia Daohua Zhuang Feng Cong Yue-Xiao Lian Metagenomic investigation of viruses in green sea turtles (Chelonia mydas) Frontiers in Microbiology green sea turtle virome metagenomics feces virus |
| title | Metagenomic investigation of viruses in green sea turtles (Chelonia mydas) |
| title_full | Metagenomic investigation of viruses in green sea turtles (Chelonia mydas) |
| title_fullStr | Metagenomic investigation of viruses in green sea turtles (Chelonia mydas) |
| title_full_unstemmed | Metagenomic investigation of viruses in green sea turtles (Chelonia mydas) |
| title_short | Metagenomic investigation of viruses in green sea turtles (Chelonia mydas) |
| title_sort | metagenomic investigation of viruses in green sea turtles chelonia mydas |
| topic | green sea turtle virome metagenomics feces virus |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1492038/full |
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