Rapid Screening of 60 Types of Natural Toxins in Whole Blood by UPLC-Q/Orbitrap HRMS
ObjectiveTo establish a rapid screening method for 60 types of natural toxins in whole blood by ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry （UPLC-Q/Orbitrap HRMS).MethodsThe chromatographic and mass spectrometric information...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Forensic Medicine
2024-12-01
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| Series: | Fayixue Zazhi |
| Subjects: | |
| Online Access: | http://www.fyxzz.cn/CN/10.12116/j.issn.1004-5619.2024.340202 |
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| Summary: | ObjectiveTo establish a rapid screening method for 60 types of natural toxins in whole blood by ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry （UPLC-Q/Orbitrap HRMS).MethodsThe chromatographic and mass spectrometric information of 60 standard samples of natural toxins were analyzed and recorded, and a screening database was built. Whole blood was pretreated by protein precipitation method combined with ultrasonic-assisted dispersion, and then a Hypersil GOLDTM C18 column was used with 5 mmol/L ammonium formate aqueous solution （containing 0.1% formic acid） and acetonitrile as mobile phase for gradient elution. In the positive ion mode, the data were collected in full scan/data-dependent secondary scan （Full MS/dd-MS2） mode. Based on the established screening library, the rapid screening of 60 types of natural toxins in whole blood was realized by TraceFinder software.ResultsA UPLC-Q/Orbitrap HRMS method was developed for the screening of 60 types of natural toxins in whole blood. Except for the limit of detection （LOD） of oxymatrine （20 ng/mL） and strophanthidin （40 ng/mL）, the LOD for the other 58 natural toxins was in the range of 0.05-5 ng/mL.ConclusionThis method has a simple and efficient pretreatment process and can achieve rapid screening of 60 types of natural toxins in whole blood. |
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| ISSN: | 1004-5619 |