Aptamer Sequence Optimization and Its Application in Food Safety Analysis
Aptamers are single-stranded DNA or RNA oligonucleotides screened by systematic evolution of ligands by exponential enrichment (SELEX) methods, which are widely used in food analysis. Aptamers have the advantages of low molecular weight, ease of preparation, simplicity of chemical modification, and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-07-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/14/15/2622 |
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| author | Xinna Qin Lina Zhao Yang Zhang Jiyong Shi Haroon Elrasheid Tahir Xuechao Xu Kaiyi Zheng Xiaobo Zou |
| author_facet | Xinna Qin Lina Zhao Yang Zhang Jiyong Shi Haroon Elrasheid Tahir Xuechao Xu Kaiyi Zheng Xiaobo Zou |
| author_sort | Xinna Qin |
| collection | DOAJ |
| description | Aptamers are single-stranded DNA or RNA oligonucleotides screened by systematic evolution of ligands by exponential enrichment (SELEX) methods, which are widely used in food analysis. Aptamers have the advantages of low molecular weight, ease of preparation, simplicity of chemical modification, and structural stability. Aptamers generated by SELEX are typically 80–100 bases in length, and the affinity of the aptamer can be improved by sequence optimization. Methods of aptamer optimization commonly include truncation, mutation, and chemical modification, and molecular docking, molecular dynamics, circular dichroism, and isothermal titration to assess often the binding performance of the aptamer to the target. Optimized aptamers usually enhance the affinity of the aptamer for the target and increase its sensitivity in the detection of pesticides, heavy metals, fungal toxins, pathogenic bacteria, and other objects. This paper focuses on truncation, mutation, chemical modification, the introduction of rare nucleotides, and computer-aided design. It provides an overview of non-immobilized optimization metrics. |
| format | Article |
| id | doaj-art-e71bed3113584a4e9548d0777d4697eb |
| institution | Kabale University |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-e71bed3113584a4e9548d0777d4697eb2025-08-20T03:36:01ZengMDPI AGFoods2304-81582025-07-011415262210.3390/foods14152622Aptamer Sequence Optimization and Its Application in Food Safety AnalysisXinna Qin0Lina Zhao1Yang Zhang2Jiyong Shi3Haroon Elrasheid Tahir4Xuechao Xu5Kaiyi Zheng6Xiaobo Zou7School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaAptamers are single-stranded DNA or RNA oligonucleotides screened by systematic evolution of ligands by exponential enrichment (SELEX) methods, which are widely used in food analysis. Aptamers have the advantages of low molecular weight, ease of preparation, simplicity of chemical modification, and structural stability. Aptamers generated by SELEX are typically 80–100 bases in length, and the affinity of the aptamer can be improved by sequence optimization. Methods of aptamer optimization commonly include truncation, mutation, and chemical modification, and molecular docking, molecular dynamics, circular dichroism, and isothermal titration to assess often the binding performance of the aptamer to the target. Optimized aptamers usually enhance the affinity of the aptamer for the target and increase its sensitivity in the detection of pesticides, heavy metals, fungal toxins, pathogenic bacteria, and other objects. This paper focuses on truncation, mutation, chemical modification, the introduction of rare nucleotides, and computer-aided design. It provides an overview of non-immobilized optimization metrics.https://www.mdpi.com/2304-8158/14/15/2622aptamersequenceoptimizationtargetdetection |
| spellingShingle | Xinna Qin Lina Zhao Yang Zhang Jiyong Shi Haroon Elrasheid Tahir Xuechao Xu Kaiyi Zheng Xiaobo Zou Aptamer Sequence Optimization and Its Application in Food Safety Analysis Foods aptamer sequence optimization target detection |
| title | Aptamer Sequence Optimization and Its Application in Food Safety Analysis |
| title_full | Aptamer Sequence Optimization and Its Application in Food Safety Analysis |
| title_fullStr | Aptamer Sequence Optimization and Its Application in Food Safety Analysis |
| title_full_unstemmed | Aptamer Sequence Optimization and Its Application in Food Safety Analysis |
| title_short | Aptamer Sequence Optimization and Its Application in Food Safety Analysis |
| title_sort | aptamer sequence optimization and its application in food safety analysis |
| topic | aptamer sequence optimization target detection |
| url | https://www.mdpi.com/2304-8158/14/15/2622 |
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