FADD is a key regulator of lipid metabolism

Abstract FADD, a classical apoptotic signaling adaptor, was recently reported to have non‐apoptotic functions. Here, we report the discovery that FADD regulates lipid metabolism. PPAR‐α is a dietary lipid sensor, whose activation results in hypolipidemic effects. We show that FADD interacts with RIP...

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Main Authors: Hongqin Zhuang, Xueshi Wang, Daolong Zha, Ziyi Gan, Fangfang Cai, Pan Du, Yunwen Yang, Bingya Yang, Xiangyu Zhang, Chun Yao, Yuqiang Zhou, Chizhou Jiang, Shengwen Guan, Xuerui Zhang, Jing Zhang, Wenhui Jiang, Qingang Hu, Zi‐Chun Hua
Format: Article
Language:English
Published: Springer Nature 2016-06-01
Series:EMBO Molecular Medicine
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Online Access:https://doi.org/10.15252/emmm.201505924
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author Hongqin Zhuang
Xueshi Wang
Daolong Zha
Ziyi Gan
Fangfang Cai
Pan Du
Yunwen Yang
Bingya Yang
Xiangyu Zhang
Chun Yao
Yuqiang Zhou
Chizhou Jiang
Shengwen Guan
Xuerui Zhang
Jing Zhang
Wenhui Jiang
Qingang Hu
Zi‐Chun Hua
author_facet Hongqin Zhuang
Xueshi Wang
Daolong Zha
Ziyi Gan
Fangfang Cai
Pan Du
Yunwen Yang
Bingya Yang
Xiangyu Zhang
Chun Yao
Yuqiang Zhou
Chizhou Jiang
Shengwen Guan
Xuerui Zhang
Jing Zhang
Wenhui Jiang
Qingang Hu
Zi‐Chun Hua
author_sort Hongqin Zhuang
collection DOAJ
description Abstract FADD, a classical apoptotic signaling adaptor, was recently reported to have non‐apoptotic functions. Here, we report the discovery that FADD regulates lipid metabolism. PPAR‐α is a dietary lipid sensor, whose activation results in hypolipidemic effects. We show that FADD interacts with RIP140, which is a corepressor for PPAR‐α, and FADD phosphorylation‐mimic mutation (FADD‐D) or FADD deficiency abolishes RIP140‐mediated transcriptional repression, leading to the activation of PPAR‐α. FADD‐D‐mutant mice exhibit significantly decreased adipose tissue mass and triglyceride accumulation. Also, they exhibit increased energy expenditure with enhanced fatty acid oxidation in adipocytes due to the activation of PPAR‐α. Similar metabolic phenotypes, such as reduced fat formation, insulin resistance, and resistance to HFD‐induced obesity, are shown in adipose‐specific FADD knockout mice. Additionally, FADD‐D mutation can reverse the severe genetic obesity phenotype of ob/ob mice, with elevated fatty acid oxidation and oxygen consumption in adipose tissue, improved insulin resistance, and decreased triglyceride storage. We conclude that FADD is a master regulator of glucose and fat metabolism with potential applications for treatment of insulin resistance and obesity.
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spelling doaj-art-e70cdb2e805b45e7a77b0401ac41530c2025-08-20T04:03:06ZengSpringer NatureEMBO Molecular Medicine1757-46761757-46842016-06-018889591810.15252/emmm.201505924FADD is a key regulator of lipid metabolismHongqin Zhuang0Xueshi Wang1Daolong Zha2Ziyi Gan3Fangfang Cai4Pan Du5Yunwen Yang6Bingya Yang7Xiangyu Zhang8Chun Yao9Yuqiang Zhou10Chizhou Jiang11Shengwen Guan12Xuerui Zhang13Jing Zhang14Wenhui Jiang15Qingang Hu16Zi‐Chun Hua17The State Key Laboratory of Pharmaceutical Biotechnology, College of Life Science and School of Stomatology, Affiliated Stomatological Hospital, Nanjing UniversityThe State Key Laboratory of Pharmaceutical Biotechnology, College of Life Science and School of Stomatology, Affiliated Stomatological Hospital, Nanjing UniversityThe State Key Laboratory of Pharmaceutical Biotechnology, College of Life Science and School of Stomatology, Affiliated Stomatological Hospital, Nanjing UniversityThe State Key Laboratory of Pharmaceutical Biotechnology, College of Life Science and School of Stomatology, Affiliated Stomatological Hospital, Nanjing UniversityThe State Key Laboratory of Pharmaceutical Biotechnology, College of Life Science and School of Stomatology, Affiliated Stomatological Hospital, Nanjing UniversityThe State Key Laboratory of Pharmaceutical Biotechnology, College of Life Science and School of Stomatology, Affiliated Stomatological Hospital, Nanjing UniversityThe State Key Laboratory of Pharmaceutical Biotechnology, College of Life Science and School of Stomatology, Affiliated Stomatological Hospital, Nanjing UniversityThe State Key Laboratory of Pharmaceutical Biotechnology, College of Life Science and School of Stomatology, Affiliated Stomatological Hospital, Nanjing UniversityThe State Key Laboratory of Pharmaceutical Biotechnology, College of Life Science and School of Stomatology, Affiliated Stomatological Hospital, Nanjing UniversityThe State Key Laboratory of Pharmaceutical Biotechnology, College of Life Science and School of Stomatology, Affiliated Stomatological Hospital, Nanjing UniversityThe State Key Laboratory of Pharmaceutical Biotechnology, College of Life Science and School of Stomatology, Affiliated Stomatological Hospital, Nanjing UniversityThe State Key Laboratory of Pharmaceutical Biotechnology, College of Life Science and School of Stomatology, Affiliated Stomatological Hospital, Nanjing UniversityChangzhou High‐Tech Research Institute of Nanjing University and Jiangsu TargetPharma Laboratories Inc.The State Key Laboratory of Pharmaceutical Biotechnology, College of Life Science and School of Stomatology, Affiliated Stomatological Hospital, Nanjing UniversityThe State Key Laboratory of Pharmaceutical Biotechnology, College of Life Science and School of Stomatology, Affiliated Stomatological Hospital, Nanjing UniversityThe State Key Laboratory of Pharmaceutical Biotechnology, College of Life Science and School of Stomatology, Affiliated Stomatological Hospital, Nanjing UniversityThe State Key Laboratory of Pharmaceutical Biotechnology, College of Life Science and School of Stomatology, Affiliated Stomatological Hospital, Nanjing UniversityThe State Key Laboratory of Pharmaceutical Biotechnology, College of Life Science and School of Stomatology, Affiliated Stomatological Hospital, Nanjing UniversityAbstract FADD, a classical apoptotic signaling adaptor, was recently reported to have non‐apoptotic functions. Here, we report the discovery that FADD regulates lipid metabolism. PPAR‐α is a dietary lipid sensor, whose activation results in hypolipidemic effects. We show that FADD interacts with RIP140, which is a corepressor for PPAR‐α, and FADD phosphorylation‐mimic mutation (FADD‐D) or FADD deficiency abolishes RIP140‐mediated transcriptional repression, leading to the activation of PPAR‐α. FADD‐D‐mutant mice exhibit significantly decreased adipose tissue mass and triglyceride accumulation. Also, they exhibit increased energy expenditure with enhanced fatty acid oxidation in adipocytes due to the activation of PPAR‐α. Similar metabolic phenotypes, such as reduced fat formation, insulin resistance, and resistance to HFD‐induced obesity, are shown in adipose‐specific FADD knockout mice. Additionally, FADD‐D mutation can reverse the severe genetic obesity phenotype of ob/ob mice, with elevated fatty acid oxidation and oxygen consumption in adipose tissue, improved insulin resistance, and decreased triglyceride storage. We conclude that FADD is a master regulator of glucose and fat metabolism with potential applications for treatment of insulin resistance and obesity.https://doi.org/10.15252/emmm.201505924FADDlipid metabolismobesityPPAR‐α
spellingShingle Hongqin Zhuang
Xueshi Wang
Daolong Zha
Ziyi Gan
Fangfang Cai
Pan Du
Yunwen Yang
Bingya Yang
Xiangyu Zhang
Chun Yao
Yuqiang Zhou
Chizhou Jiang
Shengwen Guan
Xuerui Zhang
Jing Zhang
Wenhui Jiang
Qingang Hu
Zi‐Chun Hua
FADD is a key regulator of lipid metabolism
EMBO Molecular Medicine
FADD
lipid metabolism
obesity
PPAR‐α
title FADD is a key regulator of lipid metabolism
title_full FADD is a key regulator of lipid metabolism
title_fullStr FADD is a key regulator of lipid metabolism
title_full_unstemmed FADD is a key regulator of lipid metabolism
title_short FADD is a key regulator of lipid metabolism
title_sort fadd is a key regulator of lipid metabolism
topic FADD
lipid metabolism
obesity
PPAR‐α
url https://doi.org/10.15252/emmm.201505924
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