New Occurrence of <i>Nigrospora oryzae</i> Causing Leaf Blight in <i>Ginkgo biloba</i> in China and Biocontrol Screening of Endophytic Bacteria

New Occurrence of <i>Nigrospora oryzae</i> Causing Leaf Blight in <i>Ginkgo biloba</i> in China and Biocontrol Screening of Endophytic Bacteria

<i>Ginkgo biloba</i> is a multifunctional composite tree species that has important ornamental, economic, medicinal, and scientific research value. In October 2023, the foliage of <i>G. biloba</i> on the campus of Nanjing Forestry University exhibited leaf blight. Black-brown...

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Bibliographic Details
Main Authors: Yuan Tao, Chun Yang, Sinong Yu, Fangfang Fu, Tingting Dai
Format: Article
Language:English
Published: MDPI AG 2024-10-01
Series:Microorganisms
Subjects:
plant disease
phylogenetic analysis
pathogenicity
endophytic biocontrol bacteria
Online Access:https://www.mdpi.com/2076-2607/12/11/2125
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Summary:<i>Ginkgo biloba</i> is a multifunctional composite tree species that has important ornamental, economic, medicinal, and scientific research value. In October 2023, the foliage of <i>G. biloba</i> on the campus of Nanjing Forestry University exhibited leaf blight. Black-brown necrotic spots were observed on a large number of leaves, with a disease incidence of 86%. After isolating a fungus from symptomatic leaves, pathogenicity was tested to satisfy Koch’s postulates. Using morphological features and multi-gene phylogenetic analyses of an internal transcribed spacer (ITS), elongation factor 1-alpha (<i>EF1-α</i>), and beta-tubulin (<i>β-tub</i>), the isolates YKB1-1 and YKB1-2 were identified as <i>Nigrospora oryzae. N. oryzae</i> was previously reported as an endophyte of <i>G. biloba</i>. However, this study shows it to be pathogenic to <i>G. biloba</i>, causing leaf spots. Two endophytic bacteria were isolated from asymptomatic leaves of diseased <i>G. biloba</i> trees, and their molecular identification was performed using 16S ribosomal DNA (16S rDNA). GBB1-2 was identified as <i>Bacillus altitudinis</i>, while GBB1-5 was identified as <i>Bacillus amyloliquefaciens</i>. The screening and verification of endophytic bacteria provide a new strategy for the control of <i>N. oryzae</i>.
ISSN:2076-2607

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