Single Enema Fecal Microbiota Transplantation in Cats With Chronic Enteropathy

ABSTRACT Background Chronic enteropathies (CE) are common in cats, and alterations of the intestinal microbiota might be involved in the pathogenesis. Hypothesis/Objectives To evaluate the efficacy of a single enema fecal microbiota transplantation (FMT) in improving intestinal dysbiosis and clinica...

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Main Authors: Dimitra A. Karra, Jan S. Suchodolski, Shelley J. Newman, Evgenia Flouraki, Jonathan A. Lidbury, Joerg M. Steiner, Panagiotis G. Xenoulis
Format: Article
Language:English
Published: Wiley 2025-05-01
Series:Journal of Veterinary Internal Medicine
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Online Access:https://doi.org/10.1111/jvim.70054
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author Dimitra A. Karra
Jan S. Suchodolski
Shelley J. Newman
Evgenia Flouraki
Jonathan A. Lidbury
Joerg M. Steiner
Panagiotis G. Xenoulis
author_facet Dimitra A. Karra
Jan S. Suchodolski
Shelley J. Newman
Evgenia Flouraki
Jonathan A. Lidbury
Joerg M. Steiner
Panagiotis G. Xenoulis
author_sort Dimitra A. Karra
collection DOAJ
description ABSTRACT Background Chronic enteropathies (CE) are common in cats, and alterations of the intestinal microbiota might be involved in the pathogenesis. Hypothesis/Objectives To evaluate the efficacy of a single enema fecal microbiota transplantation (FMT) in improving intestinal dysbiosis and clinical scores in cats with CE. Animals Twenty‐eight cats with either chronic inflammatory enteropathy (CIE; n = 19) or small cell gastrointestinal lymphoma (SCGL; n = 9) were prospectively enrolled. Methods Eleven cats were randomly selected to receive a single enema FMT (FMT‐group), and 17 cats were used as controls. Clinical activity was determined using the Feline Chronic Enteropathy Activity Index (FCEAI), and intestinal dysbiosis was determined using the feline dysbiosis index (DI) on the day of FMT (T0) and 30 days after FMT (T1). Results At T0, 14/28 cats had an abnormal DI > 0. No significant difference was found in the DI from T0 to T1 in the FMT group (mean[SD]: 0.01[2.5] vs. 0.7[2.1]; p = 0.47). No significant difference was found in the DI between the FMT group and the control group at T1 (mean[SD]: −0.7[2.1] vs. 0.8[1.8]; p = 0.92). FCEAI significantly decreased at T1 compared to T0 in the FMT group (median[IQR] 10.0[7.7–11.3] vs. 4.5[4–5]; p = 0.002). No significant difference was found in the FCEAI between the FMT group and the control group at T1 (median[IQR] 4.5[4–5] vs. 4[3–5.75]; p = 0.64). Conclusions In this study, single enema FMT did not lead to a significant improvement in DI or FCEAI in cats with CE compared to controls.
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spelling doaj-art-e6f99ebd681e43cdbdfc805d05fdc7c32025-08-20T01:53:50ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762025-05-01393n/an/a10.1111/jvim.70054Single Enema Fecal Microbiota Transplantation in Cats With Chronic EnteropathyDimitra A. Karra0Jan S. Suchodolski1Shelley J. Newman2Evgenia Flouraki3Jonathan A. Lidbury4Joerg M. Steiner5Panagiotis G. Xenoulis6Clinic of Medicine, Faculty of Veterinary Science University of Thessaly Karditsa GreeceGastrointestinal Laboratory, Department of Small Animal Clinical Sciences Texas A&M University Texas USANewman Specialty VetPath New York USAClinic of Surgery, Faculty of Veterinary Science University of Thessaly Karditsa GreeceGastrointestinal Laboratory, Department of Small Animal Clinical Sciences Texas A&M University Texas USAGastrointestinal Laboratory, Department of Small Animal Clinical Sciences Texas A&M University Texas USAClinic of Medicine, Faculty of Veterinary Science University of Thessaly Karditsa GreeceABSTRACT Background Chronic enteropathies (CE) are common in cats, and alterations of the intestinal microbiota might be involved in the pathogenesis. Hypothesis/Objectives To evaluate the efficacy of a single enema fecal microbiota transplantation (FMT) in improving intestinal dysbiosis and clinical scores in cats with CE. Animals Twenty‐eight cats with either chronic inflammatory enteropathy (CIE; n = 19) or small cell gastrointestinal lymphoma (SCGL; n = 9) were prospectively enrolled. Methods Eleven cats were randomly selected to receive a single enema FMT (FMT‐group), and 17 cats were used as controls. Clinical activity was determined using the Feline Chronic Enteropathy Activity Index (FCEAI), and intestinal dysbiosis was determined using the feline dysbiosis index (DI) on the day of FMT (T0) and 30 days after FMT (T1). Results At T0, 14/28 cats had an abnormal DI > 0. No significant difference was found in the DI from T0 to T1 in the FMT group (mean[SD]: 0.01[2.5] vs. 0.7[2.1]; p = 0.47). No significant difference was found in the DI between the FMT group and the control group at T1 (mean[SD]: −0.7[2.1] vs. 0.8[1.8]; p = 0.92). FCEAI significantly decreased at T1 compared to T0 in the FMT group (median[IQR] 10.0[7.7–11.3] vs. 4.5[4–5]; p = 0.002). No significant difference was found in the FCEAI between the FMT group and the control group at T1 (median[IQR] 4.5[4–5] vs. 4[3–5.75]; p = 0.64). Conclusions In this study, single enema FMT did not lead to a significant improvement in DI or FCEAI in cats with CE compared to controls.https://doi.org/10.1111/jvim.70054CIEfelineFMTlymphoma
spellingShingle Dimitra A. Karra
Jan S. Suchodolski
Shelley J. Newman
Evgenia Flouraki
Jonathan A. Lidbury
Joerg M. Steiner
Panagiotis G. Xenoulis
Single Enema Fecal Microbiota Transplantation in Cats With Chronic Enteropathy
Journal of Veterinary Internal Medicine
CIE
feline
FMT
lymphoma
title Single Enema Fecal Microbiota Transplantation in Cats With Chronic Enteropathy
title_full Single Enema Fecal Microbiota Transplantation in Cats With Chronic Enteropathy
title_fullStr Single Enema Fecal Microbiota Transplantation in Cats With Chronic Enteropathy
title_full_unstemmed Single Enema Fecal Microbiota Transplantation in Cats With Chronic Enteropathy
title_short Single Enema Fecal Microbiota Transplantation in Cats With Chronic Enteropathy
title_sort single enema fecal microbiota transplantation in cats with chronic enteropathy
topic CIE
feline
FMT
lymphoma
url https://doi.org/10.1111/jvim.70054
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AT janssuchodolski singleenemafecalmicrobiotatransplantationincatswithchronicenteropathy
AT shelleyjnewman singleenemafecalmicrobiotatransplantationincatswithchronicenteropathy
AT evgeniaflouraki singleenemafecalmicrobiotatransplantationincatswithchronicenteropathy
AT jonathanalidbury singleenemafecalmicrobiotatransplantationincatswithchronicenteropathy
AT joergmsteiner singleenemafecalmicrobiotatransplantationincatswithchronicenteropathy
AT panagiotisgxenoulis singleenemafecalmicrobiotatransplantationincatswithchronicenteropathy