A Study of Molecular Mimicry and Immunological Cross-reactivity between Hepatitis B Surface Antigen and Myelin Mimics
On the basis of the reported association between hepatitis B vaccination (HBvacc) and autoimmune demyelinating complications such as multiple sclerosis (MS), we have looked for aminoacid similarities between the small hepatitis B virus surface antigen (SHBsAg),...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2005-01-01
|
| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1080/17402520500285247 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832549270498050048 |
|---|---|
| author | Dimitrios-Petrou Bogdanos Heather Smith Yun Ma Harold Baum Giorgina Mieli-Vergani Diego Vergani |
| author_facet | Dimitrios-Petrou Bogdanos Heather Smith Yun Ma Harold Baum Giorgina Mieli-Vergani Diego Vergani |
| author_sort | Dimitrios-Petrou Bogdanos |
| collection | DOAJ |
| description | On the basis of the reported association between hepatitis B vaccination
(HBvacc) and autoimmune demyelinating complications such as multiple sclerosis
(MS), we have looked for aminoacid similarities between the small hepatitis
B virus surface antigen (SHBsAg), and the MS-autoantigens myelin basic protein
(MBP) and myelin oligodendrocyte glycoprotein (MOG) that could serve as targets
of immunological cross-reactivity. Twenty-mer peptides spanning 4 SHBsAg/MOG
and 1 SHBsAg/MBP mimicking pairs, were constructed and tested by ELISA as
targets of cross-reactive responses. A total of 147 samples from 58 adults were
collected before HBvacc (58/58), and post-HBvacc (48/58 before the second and
41/58 before the third boost). Eighty-seven sera from anti-SHBsAg antibody
negative patients with various diseases were tested as pathological controls.
Reactivity to at least one of the SHBsAg peptides was found in 8 (14%)
pre-HBvacc subjects; amongst the remaining 50, reactivity to at least one of the
SHBsAg peptides appeared in 47 (94%) post-HBvacc. Reactivity to at least one
of the MOG mimics was present in 4 (8%) pre-HBvacc and in 30 (60%)
post-HBvacc (p < 0.001). Overall 30/50 (60%) vaccinees had SHBsAg/MOG
double reactivity on at least one occasion compared to none before-vaccination
and in 2 (2%) of the pathological controls (p < 0.001 for both). SHBsAg/MOG
double reactivity was cross-reactive as confirmed by inhibition studies. At 6 months
post-vaccination, 3 of the 4 anti-MOG reactive cases before vaccination and 7
of the 24 (29%) of the anti-MOG reactive cases at 3 months post-vaccination had
lost their reactivity to MOG5-24. There was no reactivity to the SHBsAg/MBP
mimics. None of the vaccinees reported symptoms of demyelinating disorders. In
view of the observed SHBsAg/MOG cross-reactivity, the vaccine's possible role as
an immunomodulator of viral/self cross-reactivity must be further investigated. |
| format | Article |
| id | doaj-art-e6de9c739f4b41f0afcd4253adcfef18 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2005-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-e6de9c739f4b41f0afcd4253adcfef182025-02-03T06:11:46ZengWileyClinical and Developmental Immunology1740-25221740-25302005-01-0112321722410.1080/17402520500285247A Study of Molecular Mimicry and Immunological Cross-reactivity between Hepatitis B Surface Antigen and Myelin MimicsDimitrios-Petrou Bogdanos0Heather Smith1Yun Ma2Harold Baum3Giorgina Mieli-Vergani4Diego Vergani5Institute of Liver Studies, King's College London School of Medicine at King's College Hospital, Denmark Hill, London SE5 9RS, UKInstitute of Liver Studies, King's College London School of Medicine at King's College Hospital, Denmark Hill, London SE5 9RS, UKInstitute of Liver Studies, King's College London School of Medicine at King's College Hospital, Denmark Hill, London SE5 9RS, UKSchool of Biomedical and Health Sciences, King's College, London SE1 9NN, UKInstitute of Liver Studies, King's College London School of Medicine at King's College Hospital, Denmark Hill, London SE5 9RS, UKInstitute of Liver Studies, King's College London School of Medicine at King's College Hospital, Denmark Hill, London SE5 9RS, UKOn the basis of the reported association between hepatitis B vaccination (HBvacc) and autoimmune demyelinating complications such as multiple sclerosis (MS), we have looked for aminoacid similarities between the small hepatitis B virus surface antigen (SHBsAg), and the MS-autoantigens myelin basic protein (MBP) and myelin oligodendrocyte glycoprotein (MOG) that could serve as targets of immunological cross-reactivity. Twenty-mer peptides spanning 4 SHBsAg/MOG and 1 SHBsAg/MBP mimicking pairs, were constructed and tested by ELISA as targets of cross-reactive responses. A total of 147 samples from 58 adults were collected before HBvacc (58/58), and post-HBvacc (48/58 before the second and 41/58 before the third boost). Eighty-seven sera from anti-SHBsAg antibody negative patients with various diseases were tested as pathological controls. Reactivity to at least one of the SHBsAg peptides was found in 8 (14%) pre-HBvacc subjects; amongst the remaining 50, reactivity to at least one of the SHBsAg peptides appeared in 47 (94%) post-HBvacc. Reactivity to at least one of the MOG mimics was present in 4 (8%) pre-HBvacc and in 30 (60%) post-HBvacc (p < 0.001). Overall 30/50 (60%) vaccinees had SHBsAg/MOG double reactivity on at least one occasion compared to none before-vaccination and in 2 (2%) of the pathological controls (p < 0.001 for both). SHBsAg/MOG double reactivity was cross-reactive as confirmed by inhibition studies. At 6 months post-vaccination, 3 of the 4 anti-MOG reactive cases before vaccination and 7 of the 24 (29%) of the anti-MOG reactive cases at 3 months post-vaccination had lost their reactivity to MOG5-24. There was no reactivity to the SHBsAg/MBP mimics. None of the vaccinees reported symptoms of demyelinating disorders. In view of the observed SHBsAg/MOG cross-reactivity, the vaccine's possible role as an immunomodulator of viral/self cross-reactivity must be further investigated.http://dx.doi.org/10.1080/17402520500285247 |
| spellingShingle | Dimitrios-Petrou Bogdanos Heather Smith Yun Ma Harold Baum Giorgina Mieli-Vergani Diego Vergani A Study of Molecular Mimicry and Immunological Cross-reactivity between Hepatitis B Surface Antigen and Myelin Mimics Clinical and Developmental Immunology |
| title | A Study of Molecular Mimicry and Immunological Cross-reactivity between Hepatitis B Surface Antigen and Myelin Mimics |
| title_full | A Study of Molecular Mimicry and Immunological Cross-reactivity between Hepatitis B Surface Antigen and Myelin Mimics |
| title_fullStr | A Study of Molecular Mimicry and Immunological Cross-reactivity between Hepatitis B Surface Antigen and Myelin Mimics |
| title_full_unstemmed | A Study of Molecular Mimicry and Immunological Cross-reactivity between Hepatitis B Surface Antigen and Myelin Mimics |
| title_short | A Study of Molecular Mimicry and Immunological Cross-reactivity between Hepatitis B Surface Antigen and Myelin Mimics |
| title_sort | study of molecular mimicry and immunological cross reactivity between hepatitis b surface antigen and myelin mimics |
| url | http://dx.doi.org/10.1080/17402520500285247 |
| work_keys_str_mv | AT dimitriospetroubogdanos astudyofmolecularmimicryandimmunologicalcrossreactivitybetweenhepatitisbsurfaceantigenandmyelinmimics AT heathersmith astudyofmolecularmimicryandimmunologicalcrossreactivitybetweenhepatitisbsurfaceantigenandmyelinmimics AT yunma astudyofmolecularmimicryandimmunologicalcrossreactivitybetweenhepatitisbsurfaceantigenandmyelinmimics AT haroldbaum astudyofmolecularmimicryandimmunologicalcrossreactivitybetweenhepatitisbsurfaceantigenandmyelinmimics AT giorginamielivergani astudyofmolecularmimicryandimmunologicalcrossreactivitybetweenhepatitisbsurfaceantigenandmyelinmimics AT diegovergani astudyofmolecularmimicryandimmunologicalcrossreactivitybetweenhepatitisbsurfaceantigenandmyelinmimics AT dimitriospetroubogdanos studyofmolecularmimicryandimmunologicalcrossreactivitybetweenhepatitisbsurfaceantigenandmyelinmimics AT heathersmith studyofmolecularmimicryandimmunologicalcrossreactivitybetweenhepatitisbsurfaceantigenandmyelinmimics AT yunma studyofmolecularmimicryandimmunologicalcrossreactivitybetweenhepatitisbsurfaceantigenandmyelinmimics AT haroldbaum studyofmolecularmimicryandimmunologicalcrossreactivitybetweenhepatitisbsurfaceantigenandmyelinmimics AT giorginamielivergani studyofmolecularmimicryandimmunologicalcrossreactivitybetweenhepatitisbsurfaceantigenandmyelinmimics AT diegovergani studyofmolecularmimicryandimmunologicalcrossreactivitybetweenhepatitisbsurfaceantigenandmyelinmimics |