Species-Specific Gene, spt5, in the Qualitative and Quantitative Detection of Boletus reticulatus
Boletus reticulatus is a wild edible fungus with high nutritional value in Yunnan Province. In this study, B. reticulatus was used as the research object to diagnose the species characteristics. A commercial kit was used to extract the DNA of various fungi, and the quality of DNA was determined by u...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Food Quality |
| Online Access: | http://dx.doi.org/10.1155/2022/5526810 |
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| author | Zhan Lei Chen Zhang Yinjiao Li Lunzhao Yi Ying Shang |
| author_facet | Zhan Lei Chen Zhang Yinjiao Li Lunzhao Yi Ying Shang |
| author_sort | Zhan Lei |
| collection | DOAJ |
| description | Boletus reticulatus is a wild edible fungus with high nutritional value in Yunnan Province. In this study, B. reticulatus was used as the research object to diagnose the species characteristics. A commercial kit was used to extract the DNA of various fungi, and the quality of DNA was determined by using universal fungus primers. Through sequence alignment, the spt5 gene was selected as the species-specific gene of B. reticulatus. This gene was then qualitatively and quantitatively analyzed by PCR. In the qualitative detection, the spt5 amplification products were only found in B. reticulatus which proved its good specificity. Meanwhile, SYBR Green I based quantitative PCR results were highly sensitive, and the limit of detection was 0.04 ng of genomic DNA. These experiments illustrated that spt5 is an ideal species-specific gene for the quantitative and qualitative detection of B. reticulatus. This method is also suitable for the analysis of the processed samples of B. reticulatus and the determination of the adulteration of edible wild mushrooms. |
| format | Article |
| id | doaj-art-e6dbd5e768e94702a4b869ffca918693 |
| institution | Kabale University |
| issn | 1745-4557 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Food Quality |
| spelling | doaj-art-e6dbd5e768e94702a4b869ffca9186932025-02-03T01:07:01ZengWileyJournal of Food Quality1745-45572022-01-01202210.1155/2022/5526810Species-Specific Gene, spt5, in the Qualitative and Quantitative Detection of Boletus reticulatusZhan Lei0Chen Zhang1Yinjiao Li2Lunzhao Yi3Ying Shang4Faculty of Agriculture and FoodFaculty of Agriculture and FoodFaculty of Agriculture and FoodFaculty of Agriculture and FoodFaculty of Agriculture and FoodBoletus reticulatus is a wild edible fungus with high nutritional value in Yunnan Province. In this study, B. reticulatus was used as the research object to diagnose the species characteristics. A commercial kit was used to extract the DNA of various fungi, and the quality of DNA was determined by using universal fungus primers. Through sequence alignment, the spt5 gene was selected as the species-specific gene of B. reticulatus. This gene was then qualitatively and quantitatively analyzed by PCR. In the qualitative detection, the spt5 amplification products were only found in B. reticulatus which proved its good specificity. Meanwhile, SYBR Green I based quantitative PCR results were highly sensitive, and the limit of detection was 0.04 ng of genomic DNA. These experiments illustrated that spt5 is an ideal species-specific gene for the quantitative and qualitative detection of B. reticulatus. This method is also suitable for the analysis of the processed samples of B. reticulatus and the determination of the adulteration of edible wild mushrooms.http://dx.doi.org/10.1155/2022/5526810 |
| spellingShingle | Zhan Lei Chen Zhang Yinjiao Li Lunzhao Yi Ying Shang Species-Specific Gene, spt5, in the Qualitative and Quantitative Detection of Boletus reticulatus Journal of Food Quality |
| title | Species-Specific Gene, spt5, in the Qualitative and Quantitative Detection of Boletus reticulatus |
| title_full | Species-Specific Gene, spt5, in the Qualitative and Quantitative Detection of Boletus reticulatus |
| title_fullStr | Species-Specific Gene, spt5, in the Qualitative and Quantitative Detection of Boletus reticulatus |
| title_full_unstemmed | Species-Specific Gene, spt5, in the Qualitative and Quantitative Detection of Boletus reticulatus |
| title_short | Species-Specific Gene, spt5, in the Qualitative and Quantitative Detection of Boletus reticulatus |
| title_sort | species specific gene spt5 in the qualitative and quantitative detection of boletus reticulatus |
| url | http://dx.doi.org/10.1155/2022/5526810 |
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