Expression, Purification, and Evaluation of Diagnostic Potential and Immunogenicity of a Recombinant NS3 Protein from All Serotypes of Dengue Virus
Dengue is one of the major public health concerns in the world. Since all the four serotypes are actively circulating in Mexico, there is a need to develop an efficient diagnosis system to improve case management of the patients. There exist few studies evaluating the use of the NS3 protein as a pro...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Tropical Medicine |
| Online Access: | http://dx.doi.org/10.1155/2012/956875 |
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| author | Laura Mónica Álvarez-Rodríguez Angel Ramos-Ligonio José Luis Rosales-Encina María Teresa Martínez-Cázares Aurora Parissi-Crivelli Aracely López-Monteon |
| author_facet | Laura Mónica Álvarez-Rodríguez Angel Ramos-Ligonio José Luis Rosales-Encina María Teresa Martínez-Cázares Aurora Parissi-Crivelli Aracely López-Monteon |
| author_sort | Laura Mónica Álvarez-Rodríguez |
| collection | DOAJ |
| description | Dengue is one of the major public health concerns in the world. Since all the four serotypes are actively circulating in Mexico, there is a need to develop an efficient diagnosis system to improve case management of the patients. There exist few studies evaluating the use of the NS3 protein as a protective antigen against dengue virus (DENV). In this paper we show the expression of a recombinant NS3 protein from all serotypes of dengue virus (GST-DVNS3-1-4) and report a reliable “in-house detection system” for the diagnosis of dengue infection which was field-tested in a small village (Tezonapa) in the state of Veracruz, Mexico. The fusion proteins were immunogenic, inducing antibodies to be able to recognize to antigens up to a 1 : 3200 dilution. The purified proteins were used to develop an in-house detection system (ELISA) and were further tested with a panel of 239 serum samples. The in-house results were in excellent agreement with the commercial kits with κ=0.934±0.064 (95% CI = 0.808–1.061), and κ=0.872±0.048 (95% CI = 0.779–0.965) for IgM and IgG, respectively. The agreement between the NS1 antigen detection versus the rNS3 ELISA, κ=0.837±0.066 (95% CI = 0.708–0.966), was very good. Thus, these results demonstrate that recombinant NS3 proteins have potential in early diagnosis of dengue infections. |
| format | Article |
| id | doaj-art-e6d632e18c8a413389d25d6e76b00b64 |
| institution | Kabale University |
| issn | 1687-9686 1687-9694 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Tropical Medicine |
| spelling | doaj-art-e6d632e18c8a413389d25d6e76b00b642025-02-03T01:03:11ZengWileyJournal of Tropical Medicine1687-96861687-96942012-01-01201210.1155/2012/956875956875Expression, Purification, and Evaluation of Diagnostic Potential and Immunogenicity of a Recombinant NS3 Protein from All Serotypes of Dengue VirusLaura Mónica Álvarez-Rodríguez0Angel Ramos-Ligonio1José Luis Rosales-Encina2María Teresa Martínez-Cázares3Aurora Parissi-Crivelli4Aracely López-Monteon5LADISER Inmunología y Biología Molecular, Facultad de Ciencias Químicas, Universidad Veracruzana, 94340 Orizaba, VER, MexicoLADISER Inmunología y Biología Molecular, Facultad de Ciencias Químicas, Universidad Veracruzana, 94340 Orizaba, VER, MexicoLaboratorio de Biología Molecular, Departamento de Infectómica y Patogénesis Molecular, Centro de Investigaciones y de Estudios Avanzados del Instituto Politécnico Nacional, 07360 México, DF, MexicoLaboratorio Estatal de Salud Pública, Secretaría de Salud de Veracruz, 91697 Xalapa, VER, MexicoLaboratorio Estatal de Salud Pública, Secretaría de Salud de Veracruz, 91697 Xalapa, VER, MexicoLADISER Inmunología y Biología Molecular, Facultad de Ciencias Químicas, Universidad Veracruzana, 94340 Orizaba, VER, MexicoDengue is one of the major public health concerns in the world. Since all the four serotypes are actively circulating in Mexico, there is a need to develop an efficient diagnosis system to improve case management of the patients. There exist few studies evaluating the use of the NS3 protein as a protective antigen against dengue virus (DENV). In this paper we show the expression of a recombinant NS3 protein from all serotypes of dengue virus (GST-DVNS3-1-4) and report a reliable “in-house detection system” for the diagnosis of dengue infection which was field-tested in a small village (Tezonapa) in the state of Veracruz, Mexico. The fusion proteins were immunogenic, inducing antibodies to be able to recognize to antigens up to a 1 : 3200 dilution. The purified proteins were used to develop an in-house detection system (ELISA) and were further tested with a panel of 239 serum samples. The in-house results were in excellent agreement with the commercial kits with κ=0.934±0.064 (95% CI = 0.808–1.061), and κ=0.872±0.048 (95% CI = 0.779–0.965) for IgM and IgG, respectively. The agreement between the NS1 antigen detection versus the rNS3 ELISA, κ=0.837±0.066 (95% CI = 0.708–0.966), was very good. Thus, these results demonstrate that recombinant NS3 proteins have potential in early diagnosis of dengue infections.http://dx.doi.org/10.1155/2012/956875 |
| spellingShingle | Laura Mónica Álvarez-Rodríguez Angel Ramos-Ligonio José Luis Rosales-Encina María Teresa Martínez-Cázares Aurora Parissi-Crivelli Aracely López-Monteon Expression, Purification, and Evaluation of Diagnostic Potential and Immunogenicity of a Recombinant NS3 Protein from All Serotypes of Dengue Virus Journal of Tropical Medicine |
| title | Expression, Purification, and Evaluation of Diagnostic Potential and Immunogenicity of a Recombinant NS3 Protein from All Serotypes of Dengue Virus |
| title_full | Expression, Purification, and Evaluation of Diagnostic Potential and Immunogenicity of a Recombinant NS3 Protein from All Serotypes of Dengue Virus |
| title_fullStr | Expression, Purification, and Evaluation of Diagnostic Potential and Immunogenicity of a Recombinant NS3 Protein from All Serotypes of Dengue Virus |
| title_full_unstemmed | Expression, Purification, and Evaluation of Diagnostic Potential and Immunogenicity of a Recombinant NS3 Protein from All Serotypes of Dengue Virus |
| title_short | Expression, Purification, and Evaluation of Diagnostic Potential and Immunogenicity of a Recombinant NS3 Protein from All Serotypes of Dengue Virus |
| title_sort | expression purification and evaluation of diagnostic potential and immunogenicity of a recombinant ns3 protein from all serotypes of dengue virus |
| url | http://dx.doi.org/10.1155/2012/956875 |
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