Tolerance of Chemoorganotrophic Bioleaching Microorganisms to Heavy Metal and Alkaline Stresses
The bioleaching potential of the bacterium Bacillus mucilaginosus and the fungus Aspergillus niger towards industrial residues was investigated by assessing their response towards various heavy metals (including arsenic, cadmium, cobalt, chromium, nickel, lead, and zinc) and elevated pH. The plate d...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2015/861874 |
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| _version_ | 1832562137915981824 |
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| author | Annick Monballiu Nele Cardon Minh Tri Nguyen Christel Cornelly Boudewijn Meesschaert Yi Wai Chiang |
| author_facet | Annick Monballiu Nele Cardon Minh Tri Nguyen Christel Cornelly Boudewijn Meesschaert Yi Wai Chiang |
| author_sort | Annick Monballiu |
| collection | DOAJ |
| description | The bioleaching potential of the bacterium Bacillus mucilaginosus and the fungus Aspergillus niger towards industrial residues was investigated by assessing their response towards various heavy metals (including arsenic, cadmium, cobalt, chromium, nickel, lead, and zinc) and elevated pH. The plate diffusion method was performed for each metal to determine the toxicity effect. Liquid batch cultures were set up for more quantitative evaluation as well as for studying the influence of basicity. Growth curves were prepared using bacterial/fungal growth counting techniques such as plate counting, optical density measurement, and dry biomass determination. Cadmium, nickel, and arsenite had a negative influence on the growth of B. mucilaginosus, whereas A. niger was sensitive to cadmium and arsenate. However, it was shown that growth recovered when microorganisms cultured in the presence of these metals were inoculated onto metal-free medium. Based on the findings of the bacteriostatic/fungistatic effect of the metals and the adaptability of the microorganisms to fairly elevated pH values, it is concluded that both strains have potential applicability for further research concerning bioleaching of alkaline waste materials. |
| format | Article |
| id | doaj-art-e6b76c9306e7475c848ea8546545f334 |
| institution | Kabale University |
| issn | 1565-3633 1687-479X |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-e6b76c9306e7475c848ea8546545f3342025-02-03T01:23:25ZengWileyBioinorganic Chemistry and Applications1565-36331687-479X2015-01-01201510.1155/2015/861874861874Tolerance of Chemoorganotrophic Bioleaching Microorganisms to Heavy Metal and Alkaline StressesAnnick Monballiu0Nele Cardon1Minh Tri Nguyen2Christel Cornelly3Boudewijn Meesschaert4Yi Wai Chiang5Laboratory for Microbial and Biochemical Technology (Lab µBCT), Cluster for Bioengineering Technology, Department of Microbial and Molecular Systems, KU Leuven @ Brugge-Oostende (Kulab), 8400 Oostende, BelgiumLaboratory for Microbial and Biochemical Technology (Lab µBCT), Cluster for Bioengineering Technology, Department of Microbial and Molecular Systems, KU Leuven @ Brugge-Oostende (Kulab), 8400 Oostende, BelgiumMatériaux et Contrôles Physico-Chimiques, Département Mesures Physiques, Université de Bordeaux, 33175 Gradignan, FranceLaboratory for Microbial and Biochemical Technology (Lab µBCT), Cluster for Bioengineering Technology, Department of Microbial and Molecular Systems, KU Leuven @ Brugge-Oostende (Kulab), 8400 Oostende, BelgiumLaboratory for Microbial and Biochemical Technology (Lab µBCT), Cluster for Bioengineering Technology, Department of Microbial and Molecular Systems, KU Leuven @ Brugge-Oostende (Kulab), 8400 Oostende, BelgiumSchool of Engineering, University of Guelph, Guelph, ON, N1G 2W1, CanadaThe bioleaching potential of the bacterium Bacillus mucilaginosus and the fungus Aspergillus niger towards industrial residues was investigated by assessing their response towards various heavy metals (including arsenic, cadmium, cobalt, chromium, nickel, lead, and zinc) and elevated pH. The plate diffusion method was performed for each metal to determine the toxicity effect. Liquid batch cultures were set up for more quantitative evaluation as well as for studying the influence of basicity. Growth curves were prepared using bacterial/fungal growth counting techniques such as plate counting, optical density measurement, and dry biomass determination. Cadmium, nickel, and arsenite had a negative influence on the growth of B. mucilaginosus, whereas A. niger was sensitive to cadmium and arsenate. However, it was shown that growth recovered when microorganisms cultured in the presence of these metals were inoculated onto metal-free medium. Based on the findings of the bacteriostatic/fungistatic effect of the metals and the adaptability of the microorganisms to fairly elevated pH values, it is concluded that both strains have potential applicability for further research concerning bioleaching of alkaline waste materials.http://dx.doi.org/10.1155/2015/861874 |
| spellingShingle | Annick Monballiu Nele Cardon Minh Tri Nguyen Christel Cornelly Boudewijn Meesschaert Yi Wai Chiang Tolerance of Chemoorganotrophic Bioleaching Microorganisms to Heavy Metal and Alkaline Stresses Bioinorganic Chemistry and Applications |
| title | Tolerance of Chemoorganotrophic Bioleaching Microorganisms to Heavy Metal and Alkaline Stresses |
| title_full | Tolerance of Chemoorganotrophic Bioleaching Microorganisms to Heavy Metal and Alkaline Stresses |
| title_fullStr | Tolerance of Chemoorganotrophic Bioleaching Microorganisms to Heavy Metal and Alkaline Stresses |
| title_full_unstemmed | Tolerance of Chemoorganotrophic Bioleaching Microorganisms to Heavy Metal and Alkaline Stresses |
| title_short | Tolerance of Chemoorganotrophic Bioleaching Microorganisms to Heavy Metal and Alkaline Stresses |
| title_sort | tolerance of chemoorganotrophic bioleaching microorganisms to heavy metal and alkaline stresses |
| url | http://dx.doi.org/10.1155/2015/861874 |
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