Discriminative spatial localisation in confocal reflectance and carbon dots-based fluorescence imaging using mixed-mode endoscopic scanner

Discriminative spatial localisation in confocal reflectance and carbon dots-based fluorescence imaging using mixed-mode endoscopic scanner

Carbon dots (CDs)-based fluorescence imaging with confocal reflectance effectively localises labelled structures with clear sample visualization. Yet, integrating these modalities into single-fibre cantilever-based endoscopic scanners poses challenges. Thus, we present an endoscopic scanner with a m...

Full description

Saved in:
Bibliographic Details
Main Authors: Yang Sing Leong, Mohd Hadri Hafiz Mokhtar, Nur Afifah Ahmad Nazri, Mohd Hafiz Abu Bakar, Mohd Saiful Dzulkefly Zan, Norhana Arsad, Peer Mohamed Abdul, Ahmad Ashrif A. Bakar
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:International Journal of Optomechatronics
Subjects:
Lissajous scanning
confocal reflectance
fluorescence imaging
endoscopic scanner
carbon dots
Online Access:https://www.tandfonline.com/doi/10.1080/15599612.2024.2347292
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Carbon dots (CDs)-based fluorescence imaging with confocal reflectance effectively localises labelled structures with clear sample visualization. Yet, integrating these modalities into single-fibre cantilever-based endoscopic scanners poses challenges. Thus, we present an endoscopic scanner with a mixed-mode fibre cantilever for dual modalities imaging. The cantilever features two-attached fibre construction for multiple illumination and detection channels without additional optical components. The measured lateral and axial resolutions are 5.81 and 6.52 µm, respectively with field-of-view of 408 × 488 µm. The fluorescence detection capability was first verified using fluorescent microspheres and CD-embedded polymer film, followed by confocal reflectance and CDs-based fluorescence imaging on labelled fibrous network and plant samples. The results demonstrate the complementary strengths of confocal reflectance, which extracts surface profiles, and CDs-based fluorescence, which captures fluorescence signals, leading to precise localization of fluorescence signals within the sample. Switching modality schemes at the scanner’s back-end enables implementation of different modalities.
ISSN:1559-9612
1559-9620

Similar Items