Tear Film Proteome of Healthy Domestic Cats
The aim of this study was to investigate the proteins found in tear film of healthy domestic cats. Schirmer tear test strips were used to collect tear samples of twelve healthy cats, which were mixed, centrifuged, and placed in a single 1.5 mL microtube that was frozen at −20°C, until analysis by tw...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2021/8708023 |
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| author | Jéssica Fontes Veloso Paula Elisa Brandão Guedes Luciana Carvalho Lacerda Juliano Oliveira Santana Irma Yuliana Mora-Ocampo Carlos Priminho Pirovani Arianne Pontes Oriá Alexandre Dias Munhoz Renata Santiago Alberto Carlos |
| author_facet | Jéssica Fontes Veloso Paula Elisa Brandão Guedes Luciana Carvalho Lacerda Juliano Oliveira Santana Irma Yuliana Mora-Ocampo Carlos Priminho Pirovani Arianne Pontes Oriá Alexandre Dias Munhoz Renata Santiago Alberto Carlos |
| author_sort | Jéssica Fontes Veloso |
| collection | DOAJ |
| description | The aim of this study was to investigate the proteins found in tear film of healthy domestic cats. Schirmer tear test strips were used to collect tear samples of twelve healthy cats, which were mixed, centrifuged, and placed in a single 1.5 mL microtube that was frozen at −20°C, until analysis by two-dimensional polyacrylamide gel and mass spectrometry associated with high-performance liquid chromatography. The resulting spectra were analyzed and compared with the Swiss-Prot search tool. Forty peptides were detected in the analyzed protein fragments of 90 spots, with 16 proteins identified. Of these, the authors confirmed what has been already found in other studies: lactotransferrin, serum albumin, allergenic lipocalins, and neutrophil gelatinase-associated lipocalin. Others were considered novel in tear film samples of all species: cyclin-dependent protein kinase, serine/arginine repetitive matrix protein, apelin receptor, secretory protein related to C1q/TNF, Wee1, α-1,4 glucan phosphorylase, and WD repeat domain 1. The network was divided into 11 clusters, and a biological function was assigned. Most of the proteins have functions in the defense and maintenance of feline ocular surface homeostasis. Serum albumin is a bottleneck protein, with a high betweenness value. This paper is a pioneer in reporting, in-depth, the tear film proteome of domestic cats. |
| format | Article |
| id | doaj-art-e6a80860312c4b3f9b70717cfa06d42a |
| institution | Kabale University |
| issn | 2090-8113 2042-0048 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-e6a80860312c4b3f9b70717cfa06d42a2025-02-03T05:44:09ZengWileyVeterinary Medicine International2090-81132042-00482021-01-01202110.1155/2021/87080238708023Tear Film Proteome of Healthy Domestic CatsJéssica Fontes Veloso0Paula Elisa Brandão Guedes1Luciana Carvalho Lacerda2Juliano Oliveira Santana3Irma Yuliana Mora-Ocampo4Carlos Priminho Pirovani5Arianne Pontes Oriá6Alexandre Dias Munhoz7Renata Santiago Alberto Carlos8Multidisciplinary Campus of Barra, Federal University of Western Bahia, Av. 23 de Agosto, 873, Assunção, Barra, Bahia, BrazilDepartment of Agricultural and Environmental Sciences, Santa Cruz State University, Rodovia Jorge Amado, km 16, Salobrinho, Ilhéus, Bahia, BrazilDepartment of Agricultural and Environmental Sciences, Santa Cruz State University, Rodovia Jorge Amado, km 16, Salobrinho, Ilhéus, Bahia, BrazilDepartment of Biological Sciences, Santa Cruz State University, Rodovia Jorge Amado, km 16, Salobrinho, Ilhéus, Bahia, BrazilDepartment of Biological Sciences, Santa Cruz State University, Rodovia Jorge Amado, km 16, Salobrinho, Ilhéus, Bahia, BrazilDepartment of Biological Sciences, Santa Cruz State University, Rodovia Jorge Amado, km 16, Salobrinho, Ilhéus, Bahia, BrazilDepartment of Pathology and Clinics, Federal University of Bahia, Av. Adhemar de Barros, 500, Ondina, Salvador, Bahia, BrazilDepartment of Agricultural and Environmental Sciences, Santa Cruz State University, Rodovia Jorge Amado, km 16, Salobrinho, Ilhéus, Bahia, BrazilDepartment of Agricultural and Environmental Sciences, Santa Cruz State University, Rodovia Jorge Amado, km 16, Salobrinho, Ilhéus, Bahia, BrazilThe aim of this study was to investigate the proteins found in tear film of healthy domestic cats. Schirmer tear test strips were used to collect tear samples of twelve healthy cats, which were mixed, centrifuged, and placed in a single 1.5 mL microtube that was frozen at −20°C, until analysis by two-dimensional polyacrylamide gel and mass spectrometry associated with high-performance liquid chromatography. The resulting spectra were analyzed and compared with the Swiss-Prot search tool. Forty peptides were detected in the analyzed protein fragments of 90 spots, with 16 proteins identified. Of these, the authors confirmed what has been already found in other studies: lactotransferrin, serum albumin, allergenic lipocalins, and neutrophil gelatinase-associated lipocalin. Others were considered novel in tear film samples of all species: cyclin-dependent protein kinase, serine/arginine repetitive matrix protein, apelin receptor, secretory protein related to C1q/TNF, Wee1, α-1,4 glucan phosphorylase, and WD repeat domain 1. The network was divided into 11 clusters, and a biological function was assigned. Most of the proteins have functions in the defense and maintenance of feline ocular surface homeostasis. Serum albumin is a bottleneck protein, with a high betweenness value. This paper is a pioneer in reporting, in-depth, the tear film proteome of domestic cats.http://dx.doi.org/10.1155/2021/8708023 |
| spellingShingle | Jéssica Fontes Veloso Paula Elisa Brandão Guedes Luciana Carvalho Lacerda Juliano Oliveira Santana Irma Yuliana Mora-Ocampo Carlos Priminho Pirovani Arianne Pontes Oriá Alexandre Dias Munhoz Renata Santiago Alberto Carlos Tear Film Proteome of Healthy Domestic Cats Veterinary Medicine International |
| title | Tear Film Proteome of Healthy Domestic Cats |
| title_full | Tear Film Proteome of Healthy Domestic Cats |
| title_fullStr | Tear Film Proteome of Healthy Domestic Cats |
| title_full_unstemmed | Tear Film Proteome of Healthy Domestic Cats |
| title_short | Tear Film Proteome of Healthy Domestic Cats |
| title_sort | tear film proteome of healthy domestic cats |
| url | http://dx.doi.org/10.1155/2021/8708023 |
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