Decitabine Increases the Transcription of <i>RIG-I</i> Gene to Suppress the Replication of Feline Calicivirus and Canine Influenza Virus
Developing novel antiviral drugs has always been a significant forefront in biological medicine research. Antiviral drugs can be extracted, purified, and synthesized from various biological sources and by different methods. However, they are less explored in veterinary medicine for animal viruses. T...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/1/143 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587889563664384 |
|---|---|
| author | Shaotang Ye Zhen Wang Aolei Chen Ying Chen Gaoming Lou Qingmei Xie Gang Lu Shoujun Li |
| author_facet | Shaotang Ye Zhen Wang Aolei Chen Ying Chen Gaoming Lou Qingmei Xie Gang Lu Shoujun Li |
| author_sort | Shaotang Ye |
| collection | DOAJ |
| description | Developing novel antiviral drugs has always been a significant forefront in biological medicine research. Antiviral drugs can be extracted, purified, and synthesized from various biological sources and by different methods. However, they are less explored in veterinary medicine for animal viruses. This research primarily selected feline calicivirus (FCV) to screen the novel antiviral drug against animal viruses. A preliminary screening from a natural product library was conducted, with subsequent assessments to ascertain their toxicity levels and antiviral capabilities. The results showed that decitabine and alprostadil were effective in reducing FCV replication. The decitabine (5-aza-2′-deoxycytidine) was selected for antiviral mechanism investigation. Decitabine has been proven to modulate gene expression through its demethylating effect. Thus, we carried out further experiments and found that decitabine inhibited the FCV by enhancing the transcription of the feline <i>Retinoic acid-inducible gene I (RIG-I)</i> gene. Moreover, we also validated the same antiviral effect and mechanism of decitabine against the canine influenza virus (CIV). In summary, this study unveils the antiviral role of decitabine against FCV and CIV and provides evidence and novel insights into the demethylation drug-mediated antiviral effect for animal RNA viruses. |
| format | Article |
| id | doaj-art-e666fda3984a49579ef84acc1a066b94 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-e666fda3984a49579ef84acc1a066b942025-01-24T13:42:48ZengMDPI AGMicroorganisms2076-26072025-01-0113114310.3390/microorganisms13010143Decitabine Increases the Transcription of <i>RIG-I</i> Gene to Suppress the Replication of Feline Calicivirus and Canine Influenza VirusShaotang Ye0Zhen Wang1Aolei Chen2Ying Chen3Gaoming Lou4Qingmei Xie5Gang Lu6Shoujun Li7College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaHenry Fok School of Biology and Agriculture, Shaoguan University, Shaoguan 512005, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaDeveloping novel antiviral drugs has always been a significant forefront in biological medicine research. Antiviral drugs can be extracted, purified, and synthesized from various biological sources and by different methods. However, they are less explored in veterinary medicine for animal viruses. This research primarily selected feline calicivirus (FCV) to screen the novel antiviral drug against animal viruses. A preliminary screening from a natural product library was conducted, with subsequent assessments to ascertain their toxicity levels and antiviral capabilities. The results showed that decitabine and alprostadil were effective in reducing FCV replication. The decitabine (5-aza-2′-deoxycytidine) was selected for antiviral mechanism investigation. Decitabine has been proven to modulate gene expression through its demethylating effect. Thus, we carried out further experiments and found that decitabine inhibited the FCV by enhancing the transcription of the feline <i>Retinoic acid-inducible gene I (RIG-I)</i> gene. Moreover, we also validated the same antiviral effect and mechanism of decitabine against the canine influenza virus (CIV). In summary, this study unveils the antiviral role of decitabine against FCV and CIV and provides evidence and novel insights into the demethylation drug-mediated antiviral effect for animal RNA viruses.https://www.mdpi.com/2076-2607/13/1/143antiviraldecitabinefeline caliciviruscanine influenza virusRIG-I |
| spellingShingle | Shaotang Ye Zhen Wang Aolei Chen Ying Chen Gaoming Lou Qingmei Xie Gang Lu Shoujun Li Decitabine Increases the Transcription of <i>RIG-I</i> Gene to Suppress the Replication of Feline Calicivirus and Canine Influenza Virus Microorganisms antiviral decitabine feline calicivirus canine influenza virus RIG-I |
| title | Decitabine Increases the Transcription of <i>RIG-I</i> Gene to Suppress the Replication of Feline Calicivirus and Canine Influenza Virus |
| title_full | Decitabine Increases the Transcription of <i>RIG-I</i> Gene to Suppress the Replication of Feline Calicivirus and Canine Influenza Virus |
| title_fullStr | Decitabine Increases the Transcription of <i>RIG-I</i> Gene to Suppress the Replication of Feline Calicivirus and Canine Influenza Virus |
| title_full_unstemmed | Decitabine Increases the Transcription of <i>RIG-I</i> Gene to Suppress the Replication of Feline Calicivirus and Canine Influenza Virus |
| title_short | Decitabine Increases the Transcription of <i>RIG-I</i> Gene to Suppress the Replication of Feline Calicivirus and Canine Influenza Virus |
| title_sort | decitabine increases the transcription of i rig i i gene to suppress the replication of feline calicivirus and canine influenza virus |
| topic | antiviral decitabine feline calicivirus canine influenza virus RIG-I |
| url | https://www.mdpi.com/2076-2607/13/1/143 |
| work_keys_str_mv | AT shaotangye decitabineincreasesthetranscriptionofirigiigenetosuppressthereplicationoffelinecalicivirusandcanineinfluenzavirus AT zhenwang decitabineincreasesthetranscriptionofirigiigenetosuppressthereplicationoffelinecalicivirusandcanineinfluenzavirus AT aoleichen decitabineincreasesthetranscriptionofirigiigenetosuppressthereplicationoffelinecalicivirusandcanineinfluenzavirus AT yingchen decitabineincreasesthetranscriptionofirigiigenetosuppressthereplicationoffelinecalicivirusandcanineinfluenzavirus AT gaominglou decitabineincreasesthetranscriptionofirigiigenetosuppressthereplicationoffelinecalicivirusandcanineinfluenzavirus AT qingmeixie decitabineincreasesthetranscriptionofirigiigenetosuppressthereplicationoffelinecalicivirusandcanineinfluenzavirus AT ganglu decitabineincreasesthetranscriptionofirigiigenetosuppressthereplicationoffelinecalicivirusandcanineinfluenzavirus AT shoujunli decitabineincreasesthetranscriptionofirigiigenetosuppressthereplicationoffelinecalicivirusandcanineinfluenzavirus |