Isolation and characterization of Saprolegnia parasitica from cage-reared Pangasianodon hypophthalmus and its sensitivity to different antifungal compounds
Abstract Saprolegniasis is one of the most dangerous fungal diseases of fish, causing significant mortality in fish hatcheries and young ones. The present study aimed to isolate and characterize the causative fungus from fingerlings of Pangasianodon hypophthalmus cultured intensively in freshwater c...
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Nature Portfolio
2024-12-01
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| author | Sanjaykumar Karsanbhai Rathod Basanta Kumar Das Ritesh Shantilal Tandel Sohini Chatterjee Nilemesh Das Gayatri Tripathi Saurav Kumar Satyen Kumar Panda Prasanna Kumar Patil Sanjib Kumar Manna |
| author_facet | Sanjaykumar Karsanbhai Rathod Basanta Kumar Das Ritesh Shantilal Tandel Sohini Chatterjee Nilemesh Das Gayatri Tripathi Saurav Kumar Satyen Kumar Panda Prasanna Kumar Patil Sanjib Kumar Manna |
| author_sort | Sanjaykumar Karsanbhai Rathod |
| collection | DOAJ |
| description | Abstract Saprolegniasis is one of the most dangerous fungal diseases of fish, causing significant mortality in fish hatcheries and young ones. The present study aimed to isolate and characterize the causative fungus from fingerlings of Pangasianodon hypophthalmus cultured intensively in freshwater cages in Indian reservoirs and to determine minimum inhibitory concentrations of different antifungal compounds against the fungal hyphae and zoospores. The fungal isolates grown on potato dextrose agar showed an abundance of gemmae, elongated mycelia, non-septate hyphae, primary zoospores, mature zoosporangia with numerous zoospores, cysts with bundles of long hairs and were further identified as Saprolegnia parasitica following PCR amplification and sequencing of internal transcribed spacer region. S. parasitica showed temperature-sensitive optimum growth in a narrow window of 12–24 ℃, which might drive its experimental pathogenesis as well as natural infections in the winter months. In vitro sensitivity testing established negligible inhibitory activity of fluconazole, boric acid, sodium thiosulfate, and potassium permanganate while clotrimazole arrested the spore and hyphal growths at 2 mgL-1 concentration suggesting potential of the imidazole antifungal in treating S. parasitica infection in fish. The present study will serve as the baseline information for developing therapeutic and management strategies for controlling saprolegniasis in the economically significant iridescent catfish. |
| format | Article |
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| institution | DOAJ |
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| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-e65283fe4cab452fa08ccdf20e41eba22025-08-20T02:39:37ZengNature PortfolioScientific Reports2045-23222024-12-0114111210.1038/s41598-024-80075-0Isolation and characterization of Saprolegnia parasitica from cage-reared Pangasianodon hypophthalmus and its sensitivity to different antifungal compoundsSanjaykumar Karsanbhai Rathod0Basanta Kumar Das1Ritesh Shantilal Tandel2Sohini Chatterjee3Nilemesh Das4Gayatri Tripathi5Saurav Kumar6Satyen Kumar Panda7Prasanna Kumar Patil8Sanjib Kumar Manna9ICAR-Central Institute of Fisheries EducationICAR-Central Inland Fisheries Research InstituteICAR-Central Institute of Brackishwater AquacultureICAR-Central Inland Fisheries Research InstituteICAR-Central Inland Fisheries Research InstituteICAR-Central Institute of Fisheries EducationICAR-Central Institute of Fisheries EducationFood Safety and Standard Authority of IndiaICAR-Central Institute of Brackishwater AquacultureICAR-Central Inland Fisheries Research InstituteAbstract Saprolegniasis is one of the most dangerous fungal diseases of fish, causing significant mortality in fish hatcheries and young ones. The present study aimed to isolate and characterize the causative fungus from fingerlings of Pangasianodon hypophthalmus cultured intensively in freshwater cages in Indian reservoirs and to determine minimum inhibitory concentrations of different antifungal compounds against the fungal hyphae and zoospores. The fungal isolates grown on potato dextrose agar showed an abundance of gemmae, elongated mycelia, non-septate hyphae, primary zoospores, mature zoosporangia with numerous zoospores, cysts with bundles of long hairs and were further identified as Saprolegnia parasitica following PCR amplification and sequencing of internal transcribed spacer region. S. parasitica showed temperature-sensitive optimum growth in a narrow window of 12–24 ℃, which might drive its experimental pathogenesis as well as natural infections in the winter months. In vitro sensitivity testing established negligible inhibitory activity of fluconazole, boric acid, sodium thiosulfate, and potassium permanganate while clotrimazole arrested the spore and hyphal growths at 2 mgL-1 concentration suggesting potential of the imidazole antifungal in treating S. parasitica infection in fish. The present study will serve as the baseline information for developing therapeutic and management strategies for controlling saprolegniasis in the economically significant iridescent catfish.https://doi.org/10.1038/s41598-024-80075-0Pangasianodon hypophthlamusSaprolegnia parasiticaCage cultureAntifungal sensitivityTemperature |
| spellingShingle | Sanjaykumar Karsanbhai Rathod Basanta Kumar Das Ritesh Shantilal Tandel Sohini Chatterjee Nilemesh Das Gayatri Tripathi Saurav Kumar Satyen Kumar Panda Prasanna Kumar Patil Sanjib Kumar Manna Isolation and characterization of Saprolegnia parasitica from cage-reared Pangasianodon hypophthalmus and its sensitivity to different antifungal compounds Scientific Reports Pangasianodon hypophthlamus Saprolegnia parasitica Cage culture Antifungal sensitivity Temperature |
| title | Isolation and characterization of Saprolegnia parasitica from cage-reared Pangasianodon hypophthalmus and its sensitivity to different antifungal compounds |
| title_full | Isolation and characterization of Saprolegnia parasitica from cage-reared Pangasianodon hypophthalmus and its sensitivity to different antifungal compounds |
| title_fullStr | Isolation and characterization of Saprolegnia parasitica from cage-reared Pangasianodon hypophthalmus and its sensitivity to different antifungal compounds |
| title_full_unstemmed | Isolation and characterization of Saprolegnia parasitica from cage-reared Pangasianodon hypophthalmus and its sensitivity to different antifungal compounds |
| title_short | Isolation and characterization of Saprolegnia parasitica from cage-reared Pangasianodon hypophthalmus and its sensitivity to different antifungal compounds |
| title_sort | isolation and characterization of saprolegnia parasitica from cage reared pangasianodon hypophthalmus and its sensitivity to different antifungal compounds |
| topic | Pangasianodon hypophthlamus Saprolegnia parasitica Cage culture Antifungal sensitivity Temperature |
| url | https://doi.org/10.1038/s41598-024-80075-0 |
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