Crown Gall Induced by a Natural Isolate of <i>Brucella</i> (<i>Ochrobactrum</i>) <i>pseudogrignonense</i> Containing a Tumor-Inducing Plasmid
Crown gall disease in plants is caused by “Agrobacteria”, bacteria belonging to the <i>Rhizobiaceae</i> family, which carry a tumor-inducing (Ti) plasmid. Unexpectedly, we found evidence that a natural isolate from a rose crown gall, called NBC51/LBA8980, was a bacterium that did not bel...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/1/102 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587889345560576 |
|---|---|
| author | Marjolein J. G. Hooykaas Paul J. J. Hooykaas |
| author_facet | Marjolein J. G. Hooykaas Paul J. J. Hooykaas |
| author_sort | Marjolein J. G. Hooykaas |
| collection | DOAJ |
| description | Crown gall disease in plants is caused by “Agrobacteria”, bacteria belonging to the <i>Rhizobiaceae</i> family, which carry a tumor-inducing (Ti) plasmid. Unexpectedly, we found evidence that a natural isolate from a rose crown gall, called NBC51/LBA8980, was a bacterium that did not belong to the <i>Rhizobiaceae</i> family. Whole-genome sequencing revealed that this bacterium contained three large DNA circles with rRNA and tRNA genes, representing one chromosome and two chromids, respectively, and two megaplasmids, including a Ti plasmid. Average nucleotide identity (ANIb, ANIm) and genome-to-genome distance (GGDC) values above the thresholds of 96% and 70%, respectively, showed that NBC51/LBA8980 belonged to the species <i>Brucella</i> (<i>Ochrobactrum) pseudogrignonense.</i> Its Ti plasmid was almost identical to certain succinamopine Ti plasmids previously identified in <i>Agrobacterium</i> strains, suggesting that this Ti plasmid may have been recently acquired by NBC51/LBA8980 in the tumor environment. |
| format | Article |
| id | doaj-art-e641e16a03ab40d5aaaaf3c814853fee |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-e641e16a03ab40d5aaaaf3c814853fee2025-01-24T13:42:39ZengMDPI AGMicroorganisms2076-26072025-01-0113110210.3390/microorganisms13010102Crown Gall Induced by a Natural Isolate of <i>Brucella</i> (<i>Ochrobactrum</i>) <i>pseudogrignonense</i> Containing a Tumor-Inducing PlasmidMarjolein J. G. Hooykaas0Paul J. J. Hooykaas1Institute Biology Leiden (IBL), Leiden University, 2333 BE Leiden, The NetherlandsInstitute Biology Leiden (IBL), Leiden University, 2333 BE Leiden, The NetherlandsCrown gall disease in plants is caused by “Agrobacteria”, bacteria belonging to the <i>Rhizobiaceae</i> family, which carry a tumor-inducing (Ti) plasmid. Unexpectedly, we found evidence that a natural isolate from a rose crown gall, called NBC51/LBA8980, was a bacterium that did not belong to the <i>Rhizobiaceae</i> family. Whole-genome sequencing revealed that this bacterium contained three large DNA circles with rRNA and tRNA genes, representing one chromosome and two chromids, respectively, and two megaplasmids, including a Ti plasmid. Average nucleotide identity (ANIb, ANIm) and genome-to-genome distance (GGDC) values above the thresholds of 96% and 70%, respectively, showed that NBC51/LBA8980 belonged to the species <i>Brucella</i> (<i>Ochrobactrum) pseudogrignonense.</i> Its Ti plasmid was almost identical to certain succinamopine Ti plasmids previously identified in <i>Agrobacterium</i> strains, suggesting that this Ti plasmid may have been recently acquired by NBC51/LBA8980 in the tumor environment.https://www.mdpi.com/2076-2607/13/1/102crown gall<i>Agrobacterium</i><i>Brucella</i><i>Ochrobactrum</i>Ti plasmidsuccinamopine type Ti |
| spellingShingle | Marjolein J. G. Hooykaas Paul J. J. Hooykaas Crown Gall Induced by a Natural Isolate of <i>Brucella</i> (<i>Ochrobactrum</i>) <i>pseudogrignonense</i> Containing a Tumor-Inducing Plasmid Microorganisms crown gall <i>Agrobacterium</i> <i>Brucella</i> <i>Ochrobactrum</i> Ti plasmid succinamopine type Ti |
| title | Crown Gall Induced by a Natural Isolate of <i>Brucella</i> (<i>Ochrobactrum</i>) <i>pseudogrignonense</i> Containing a Tumor-Inducing Plasmid |
| title_full | Crown Gall Induced by a Natural Isolate of <i>Brucella</i> (<i>Ochrobactrum</i>) <i>pseudogrignonense</i> Containing a Tumor-Inducing Plasmid |
| title_fullStr | Crown Gall Induced by a Natural Isolate of <i>Brucella</i> (<i>Ochrobactrum</i>) <i>pseudogrignonense</i> Containing a Tumor-Inducing Plasmid |
| title_full_unstemmed | Crown Gall Induced by a Natural Isolate of <i>Brucella</i> (<i>Ochrobactrum</i>) <i>pseudogrignonense</i> Containing a Tumor-Inducing Plasmid |
| title_short | Crown Gall Induced by a Natural Isolate of <i>Brucella</i> (<i>Ochrobactrum</i>) <i>pseudogrignonense</i> Containing a Tumor-Inducing Plasmid |
| title_sort | crown gall induced by a natural isolate of i brucella i i ochrobactrum i i pseudogrignonense i containing a tumor inducing plasmid |
| topic | crown gall <i>Agrobacterium</i> <i>Brucella</i> <i>Ochrobactrum</i> Ti plasmid succinamopine type Ti |
| url | https://www.mdpi.com/2076-2607/13/1/102 |
| work_keys_str_mv | AT marjoleinjghooykaas crowngallinducedbyanaturalisolateofibrucellaiiochrobactrumiipseudogrignonenseicontainingatumorinducingplasmid AT pauljjhooykaas crowngallinducedbyanaturalisolateofibrucellaiiochrobactrumiipseudogrignonenseicontainingatumorinducingplasmid |