Crown Gall Induced by a Natural Isolate of <i>Brucella</i> (<i>Ochrobactrum</i>) <i>pseudogrignonense</i> Containing a Tumor-Inducing Plasmid
Crown gall disease in plants is caused by “Agrobacteria”, bacteria belonging to the <i>Rhizobiaceae</i> family, which carry a tumor-inducing (Ti) plasmid. Unexpectedly, we found evidence that a natural isolate from a rose crown gall, called NBC51/LBA8980, was a bacterium that did not bel...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/1/102 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Crown gall disease in plants is caused by “Agrobacteria”, bacteria belonging to the <i>Rhizobiaceae</i> family, which carry a tumor-inducing (Ti) plasmid. Unexpectedly, we found evidence that a natural isolate from a rose crown gall, called NBC51/LBA8980, was a bacterium that did not belong to the <i>Rhizobiaceae</i> family. Whole-genome sequencing revealed that this bacterium contained three large DNA circles with rRNA and tRNA genes, representing one chromosome and two chromids, respectively, and two megaplasmids, including a Ti plasmid. Average nucleotide identity (ANIb, ANIm) and genome-to-genome distance (GGDC) values above the thresholds of 96% and 70%, respectively, showed that NBC51/LBA8980 belonged to the species <i>Brucella</i> (<i>Ochrobactrum) pseudogrignonense.</i> Its Ti plasmid was almost identical to certain succinamopine Ti plasmids previously identified in <i>Agrobacterium</i> strains, suggesting that this Ti plasmid may have been recently acquired by NBC51/LBA8980 in the tumor environment. |
|---|---|
| ISSN: | 2076-2607 |