Structural dynamics of RAF1-HSP90-CDC37 and HSP90 complexes reveal asymmetric client interactions and key structural elements
Abstract RAF kinases are integral to the RAS-MAPK signaling pathway, and proper RAF1 folding relies on its interaction with the chaperone HSP90 and the cochaperone CDC37. Understanding the intricate molecular interactions governing RAF1 folding is crucial for comprehending this process. Here, we pre...
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Nature Portfolio
2024-03-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-024-05959-3 |
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| author | Lorenzo I. Finci Mayukh Chakrabarti Gulcin Gulten Joseph Finney Carissa Grose Tara Fox Renbin Yang Dwight V. Nissley Frank McCormick Dominic Esposito Trent E. Balius Dhirendra K. Simanshu |
| author_facet | Lorenzo I. Finci Mayukh Chakrabarti Gulcin Gulten Joseph Finney Carissa Grose Tara Fox Renbin Yang Dwight V. Nissley Frank McCormick Dominic Esposito Trent E. Balius Dhirendra K. Simanshu |
| author_sort | Lorenzo I. Finci |
| collection | DOAJ |
| description | Abstract RAF kinases are integral to the RAS-MAPK signaling pathway, and proper RAF1 folding relies on its interaction with the chaperone HSP90 and the cochaperone CDC37. Understanding the intricate molecular interactions governing RAF1 folding is crucial for comprehending this process. Here, we present a cryo-EM structure of the closed-state RAF1-HSP90-CDC37 complex, where the C-lobe of the RAF1 kinase domain binds to one side of the HSP90 dimer, and an unfolded N-lobe segment of the RAF1 kinase domain threads through the center of the HSP90 dimer. CDC37 binds to the kinase C-lobe, mimicking the N-lobe with its HxNI motif. We also describe structures of HSP90 dimers without RAF1 and CDC37, displaying only N-terminal and middle domains, which we term the semi-open state. Employing 1 μs atomistic simulations, energetic decomposition, and comparative structural analysis, we elucidate the dynamics and interactions within these complexes. Our quantitative analysis reveals that CDC37 bridges the HSP90-RAF1 interaction, RAF1 binds HSP90 asymmetrically, and that HSP90 structural elements engage RAF1’s unfolded region. Additionally, N- and C-terminal interactions stabilize HSP90 dimers, and molecular interactions in HSP90 dimers rearrange between the closed and semi-open states. Our findings provide valuable insight into the contributions of HSP90 and CDC37 in mediating client folding. |
| format | Article |
| id | doaj-art-e615e38499a94c368dff4f9a7c76932e |
| institution | Kabale University |
| issn | 2399-3642 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj-art-e615e38499a94c368dff4f9a7c76932e2025-02-02T12:37:31ZengNature PortfolioCommunications Biology2399-36422024-03-017111810.1038/s42003-024-05959-3Structural dynamics of RAF1-HSP90-CDC37 and HSP90 complexes reveal asymmetric client interactions and key structural elementsLorenzo I. Finci0Mayukh Chakrabarti1Gulcin Gulten2Joseph Finney3Carissa Grose4Tara Fox5Renbin Yang6Dwight V. Nissley7Frank McCormick8Dominic Esposito9Trent E. Balius10Dhirendra K. Simanshu11NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer ResearchNCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer ResearchNCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer ResearchNational Cryo-EM Facility, Cancer Research Technology Program, Frederick National Laboratory for Cancer ResearchNCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer ResearchNational Cryo-EM Facility, Cancer Research Technology Program, Frederick National Laboratory for Cancer ResearchCenter for Molecular Microscopy, Cancer Research Technology Program, Frederick National Laboratory for Cancer ResearchNCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer ResearchNCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer ResearchNCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer ResearchNCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer ResearchNCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer ResearchAbstract RAF kinases are integral to the RAS-MAPK signaling pathway, and proper RAF1 folding relies on its interaction with the chaperone HSP90 and the cochaperone CDC37. Understanding the intricate molecular interactions governing RAF1 folding is crucial for comprehending this process. Here, we present a cryo-EM structure of the closed-state RAF1-HSP90-CDC37 complex, where the C-lobe of the RAF1 kinase domain binds to one side of the HSP90 dimer, and an unfolded N-lobe segment of the RAF1 kinase domain threads through the center of the HSP90 dimer. CDC37 binds to the kinase C-lobe, mimicking the N-lobe with its HxNI motif. We also describe structures of HSP90 dimers without RAF1 and CDC37, displaying only N-terminal and middle domains, which we term the semi-open state. Employing 1 μs atomistic simulations, energetic decomposition, and comparative structural analysis, we elucidate the dynamics and interactions within these complexes. Our quantitative analysis reveals that CDC37 bridges the HSP90-RAF1 interaction, RAF1 binds HSP90 asymmetrically, and that HSP90 structural elements engage RAF1’s unfolded region. Additionally, N- and C-terminal interactions stabilize HSP90 dimers, and molecular interactions in HSP90 dimers rearrange between the closed and semi-open states. Our findings provide valuable insight into the contributions of HSP90 and CDC37 in mediating client folding.https://doi.org/10.1038/s42003-024-05959-3 |
| spellingShingle | Lorenzo I. Finci Mayukh Chakrabarti Gulcin Gulten Joseph Finney Carissa Grose Tara Fox Renbin Yang Dwight V. Nissley Frank McCormick Dominic Esposito Trent E. Balius Dhirendra K. Simanshu Structural dynamics of RAF1-HSP90-CDC37 and HSP90 complexes reveal asymmetric client interactions and key structural elements Communications Biology |
| title | Structural dynamics of RAF1-HSP90-CDC37 and HSP90 complexes reveal asymmetric client interactions and key structural elements |
| title_full | Structural dynamics of RAF1-HSP90-CDC37 and HSP90 complexes reveal asymmetric client interactions and key structural elements |
| title_fullStr | Structural dynamics of RAF1-HSP90-CDC37 and HSP90 complexes reveal asymmetric client interactions and key structural elements |
| title_full_unstemmed | Structural dynamics of RAF1-HSP90-CDC37 and HSP90 complexes reveal asymmetric client interactions and key structural elements |
| title_short | Structural dynamics of RAF1-HSP90-CDC37 and HSP90 complexes reveal asymmetric client interactions and key structural elements |
| title_sort | structural dynamics of raf1 hsp90 cdc37 and hsp90 complexes reveal asymmetric client interactions and key structural elements |
| url | https://doi.org/10.1038/s42003-024-05959-3 |
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