Testosterone and 17β-estradiol regulate hippocampal area CA3 sharp waves in male and female rats

Summary: Sharp wave-ripples (SPW-Rs) are critical to hippocampal function, and the same is true of gonadal steroids, but the interactions are unclear. We find that surgical removal of the gonads greatly reduces SPW rates in both sexes. Ripples are greatly reduced also. Testosterone treatment rescues...

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Main Authors: Patrice Pearce, John J. LaFrancois, Vanessa Skucas, Daniel Friedman, André A. Fenton, Dino Dvorak, Neil J. MacLusky, Helen E. Scharfman
Format: Article
Language:English
Published: Elsevier 2025-07-01
Series:Cell Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S2211124725007235
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author Patrice Pearce
John J. LaFrancois
Vanessa Skucas
Daniel Friedman
André A. Fenton
Dino Dvorak
Neil J. MacLusky
Helen E. Scharfman
author_facet Patrice Pearce
John J. LaFrancois
Vanessa Skucas
Daniel Friedman
André A. Fenton
Dino Dvorak
Neil J. MacLusky
Helen E. Scharfman
author_sort Patrice Pearce
collection DOAJ
description Summary: Sharp wave-ripples (SPW-Rs) are critical to hippocampal function, and the same is true of gonadal steroids, but the interactions are unclear. We find that surgical removal of the gonads greatly reduces SPW rates in both sexes. Ripples are greatly reduced also. Testosterone treatment rescues SPW and ripple rates in males, and 17β-estradiol restores SPW rates in females. We also find that male SPW rates are higher than females but have less power. Furthermore, in intact females, SPW rates fluctuate with the stage of the ovarian cycle. These data demonstrate that hippocampal SPWs are significantly affected by gonadal removal, testosterone, and 17β-estradiol. In addition, there are sex differences. The data are consistent with past demonstrations that testosterone and 17β-estradiol play central roles in hippocampus and significantly expand the views of hormone action and SPW-Rs.
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publishDate 2025-07-01
publisher Elsevier
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series Cell Reports
spelling doaj-art-e6115b6fda4b46b7b70e7a021bea2f4d2025-08-20T03:17:24ZengElsevierCell Reports2211-12472025-07-0144711595210.1016/j.celrep.2025.115952Testosterone and 17β-estradiol regulate hippocampal area CA3 sharp waves in male and female ratsPatrice Pearce0John J. LaFrancois1Vanessa Skucas2Daniel Friedman3André A. Fenton4Dino Dvorak5Neil J. MacLusky6Helen E. Scharfman7The Nathan Kline Institute for Psychiatric Research, Orangeburg, NY 10962, USA; New York University Grossman School of Medicine, New York, NY 10016, USAThe Nathan Kline Institute for Psychiatric Research, Orangeburg, NY 10962, USAThe Nathan Kline Institute for Psychiatric Research, Orangeburg, NY 10962, USANew York University Grossman School of Medicine, New York, NY 10016, USANew York University Grossman School of Medicine, New York, NY 10016, USA; New York University, New York, NY 10012, USANew York University Grossman School of Medicine, New York, NY 10016, USA; Gilgamesh Pharmaceuticals, New York, NY 10003, USAUniversity of Guelph, Guelph, ON, CanadaThe Nathan Kline Institute for Psychiatric Research, Orangeburg, NY 10962, USA; New York University Grossman School of Medicine, New York, NY 10016, USA; Corresponding authorSummary: Sharp wave-ripples (SPW-Rs) are critical to hippocampal function, and the same is true of gonadal steroids, but the interactions are unclear. We find that surgical removal of the gonads greatly reduces SPW rates in both sexes. Ripples are greatly reduced also. Testosterone treatment rescues SPW and ripple rates in males, and 17β-estradiol restores SPW rates in females. We also find that male SPW rates are higher than females but have less power. Furthermore, in intact females, SPW rates fluctuate with the stage of the ovarian cycle. These data demonstrate that hippocampal SPWs are significantly affected by gonadal removal, testosterone, and 17β-estradiol. In addition, there are sex differences. The data are consistent with past demonstrations that testosterone and 17β-estradiol play central roles in hippocampus and significantly expand the views of hormone action and SPW-Rs.http://www.sciencedirect.com/science/article/pii/S2211124725007235CP: Neuroscience
spellingShingle Patrice Pearce
John J. LaFrancois
Vanessa Skucas
Daniel Friedman
André A. Fenton
Dino Dvorak
Neil J. MacLusky
Helen E. Scharfman
Testosterone and 17β-estradiol regulate hippocampal area CA3 sharp waves in male and female rats
Cell Reports
CP: Neuroscience
title Testosterone and 17β-estradiol regulate hippocampal area CA3 sharp waves in male and female rats
title_full Testosterone and 17β-estradiol regulate hippocampal area CA3 sharp waves in male and female rats
title_fullStr Testosterone and 17β-estradiol regulate hippocampal area CA3 sharp waves in male and female rats
title_full_unstemmed Testosterone and 17β-estradiol regulate hippocampal area CA3 sharp waves in male and female rats
title_short Testosterone and 17β-estradiol regulate hippocampal area CA3 sharp waves in male and female rats
title_sort testosterone and 17β estradiol regulate hippocampal area ca3 sharp waves in male and female rats
topic CP: Neuroscience
url http://www.sciencedirect.com/science/article/pii/S2211124725007235
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