Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloproteinases and Their Inhibitors
Evening primrose (Oenothera paradoxa Hudziok) seeds are a rich source of not only a valuable oil containing an essential fatty acid - ᵧ-linolenic acid (GLA) - but also polyphenols which can be obtained from the biomass remaining after oil pressing. The aim of our studies was to evaluate the influenc...
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Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2014-09-01
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| Series: | Polish Journal of Food and Nutrition Sciences |
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| Online Access: | http://www.degruyter.com/view/j/pjfns.2014.64.issue-3/pjfns-2013-0003/pjfns-2013-0003.xml?format=INT |
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| author | Szewczyk Karolina Lewandowska Urszula Owczarek Katarzyna Sosnowska Dorota Gorlach Sylwia Koziołkiewicz Maria Hrabec Zbigniew Hrabec Elżbieta |
| author_facet | Szewczyk Karolina Lewandowska Urszula Owczarek Katarzyna Sosnowska Dorota Gorlach Sylwia Koziołkiewicz Maria Hrabec Zbigniew Hrabec Elżbieta |
| author_sort | Szewczyk Karolina |
| collection | DOAJ |
| description | Evening primrose (Oenothera paradoxa Hudziok) seeds are a rich source of not only a valuable oil containing an essential fatty acid - ᵧ-linolenic acid (GLA) - but also polyphenols which can be obtained from the biomass remaining after oil pressing. The aim of our studies was to evaluate the influence of a polyphenol extract from defatted seeds of evening primrose on human colorectal adenocarcinoma Caco-2 cell proliferation and matrix metalloproteinases (MMPs) synthesis and activity. To assess the effect of evening primrose extract on Caco-2 cell proliferation, crystal violet staining and sulforhodamine B (SRB) assays were used whereas mRNA expression and activity of MMPs were evaluated by RT-PCR and gelatin zymography. |
| format | Article |
| id | doaj-art-e60f747881c64fa0890cf14d14308afd |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2014-09-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-e60f747881c64fa0890cf14d14308afd2025-02-02T22:29:25ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072014-09-0164318119110.2478/pjfns-2013-0003pjfns-2013-0003Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloproteinases and Their InhibitorsSzewczyk Karolina0Lewandowska Urszula1Owczarek Katarzyna2Sosnowska Dorota3Gorlach Sylwia4Koziołkiewicz Maria5Hrabec Zbigniew6Hrabec Elżbieta7Department of Medical Enzymology, Medical University of Lodz, Mazowiecka 6/8, 92–215 Lodz, PolandDepartment of Biochemistry, Medical Univeristy of Lodz, Mazowiecka 6/8, 92-215 Lodz, PolandDepartment of Biochemistry, Medical Univeristy of Lodz, Mazowiecka 6/8, 92-215 Lodz, PolandInstitute of Technical Biochemistry, Technical University of Lodz, Stefanowskiego 4/10, 90–924 Lodz, PolandInstitute of Technical Biochemistry, Technical University of Lodz, Stefanowskiego 4/10, 90–924 Lodz, PolandInstitute of Technical Biochemistry, Technical University of Lodz, Stefanowskiego 4/10, 90–924 Lodz, PolandDepartment of Medical Enzymology, Medical University of Lodz, Mazowiecka 6/8, 92–215 Lodz, PolandDepartment of Medical Enzymology, Medical University of Lodz, Mazowiecka 6/8, 92–215 Lodz, PolandEvening primrose (Oenothera paradoxa Hudziok) seeds are a rich source of not only a valuable oil containing an essential fatty acid - ᵧ-linolenic acid (GLA) - but also polyphenols which can be obtained from the biomass remaining after oil pressing. The aim of our studies was to evaluate the influence of a polyphenol extract from defatted seeds of evening primrose on human colorectal adenocarcinoma Caco-2 cell proliferation and matrix metalloproteinases (MMPs) synthesis and activity. To assess the effect of evening primrose extract on Caco-2 cell proliferation, crystal violet staining and sulforhodamine B (SRB) assays were used whereas mRNA expression and activity of MMPs were evaluated by RT-PCR and gelatin zymography.http://www.degruyter.com/view/j/pjfns.2014.64.issue-3/pjfns-2013-0003/pjfns-2013-0003.xml?format=INTevening primrosematrix metalloproteinasescancer |
| spellingShingle | Szewczyk Karolina Lewandowska Urszula Owczarek Katarzyna Sosnowska Dorota Gorlach Sylwia Koziołkiewicz Maria Hrabec Zbigniew Hrabec Elżbieta Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloproteinases and Their Inhibitors Polish Journal of Food and Nutrition Sciences evening primrose matrix metalloproteinases cancer |
| title | Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloproteinases and Their Inhibitors |
| title_full | Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloproteinases and Their Inhibitors |
| title_fullStr | Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloproteinases and Their Inhibitors |
| title_full_unstemmed | Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloproteinases and Their Inhibitors |
| title_short | Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloproteinases and Their Inhibitors |
| title_sort | influence of polyphenol extract from evening primrose oenothera paradoxa seeds on proliferation of caco 2 cells and on expression synthesis and activity of matrix metalloproteinases and their inhibitors |
| topic | evening primrose matrix metalloproteinases cancer |
| url | http://www.degruyter.com/view/j/pjfns.2014.64.issue-3/pjfns-2013-0003/pjfns-2013-0003.xml?format=INT |
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