Supplementing exogenous xylanase improves the liver antioxidant capacity and immune response of Tibetan sheep fed wheat-based diets
Xylanase is a heteropolysaccharide found in the plant cell wall, which aids in the absorption and utilisation of nutrients. When embedded in animal feed, it enhances nutrient availability, leading to improved animal performance. The aim of this study was to explore the effect of xylanase supplementa...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Italian Journal of Animal Science |
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| Online Access: | http://dx.doi.org/10.1080/1828051X.2024.2411413 |
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| author | Feng-Shuo Zhang Bo-Yan Ma Zhan-Hong Gao Zhen-Ling Wu Yang-Ang-Mao Qu Yu Zhang Sheng-Zhen Hou Lin-Sheng Gui |
| author_facet | Feng-Shuo Zhang Bo-Yan Ma Zhan-Hong Gao Zhen-Ling Wu Yang-Ang-Mao Qu Yu Zhang Sheng-Zhen Hou Lin-Sheng Gui |
| author_sort | Feng-Shuo Zhang |
| collection | DOAJ |
| description | Xylanase is a heteropolysaccharide found in the plant cell wall, which aids in the absorption and utilisation of nutrients. When embedded in animal feed, it enhances nutrient availability, leading to improved animal performance. The aim of this study was to explore the effect of xylanase supplementation in wheat-based feeds on the hepatic development, antioxidant capacity, and immune response in Tibetan sheep. Sixty healthy, weaned, non-neutered male lambs (19.35 ± 2.18 kg) were randomly assigned to two equal groups (n = 30 per group). Dietary treatments were: basal diet (H group), and basal diet with 0.2% xylanase supplementation (E group). After a 10–day adaptation period, the feeding trial was carried out for 90 days. The live weight, morphology, antioxidant capacity and immune response were measured. The differential genes in liver were identified by RNA-sequencing. Our results showed that the levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were significantly higher in E group than in H group (p < 0.05). Enzyme-linked immunosorbent assay (ELISA) revealed that immunoglobulin A (IgA), IgM, and IgG were significantly higher in E group than in H group (p < 0.05). The hepatocytes of sheep fed xylanase diet showed arranged radially into hepatic plates cantered around the central vein, and the plates linked with each other to form a lost-like structure. A total of 4,509 differential genes (DEGs) were identified, among which 4,427 were up-regulated and 82 down-regulated. Additionally, the expression of antioxidant-related genes in cluding catalase (CAT), superoxide dismutase 1 (SOD1), oxidation resistance 1 (OXR1) and glutathione peroxidase 2 (GPX2), and immunity-related gene including mitogen-activated protein kinase 1 (MAPK1), signal transducer and activator of transcription 5B (STAT5B), janus kinase 3 (JAK3) and jun proto-oncogene (JUN) were verified using the quantitative reverse transcription polymerase chain reaction (qRT-PCR). Conclusion: In conclusion, dietary 0.2% xylanase supplementation promoted hepatic antioxidant capacity and immune response via modulating the expression of functional genes in Tibetan sheep. |
| format | Article |
| id | doaj-art-e5fe0a43defa44df811ee6fc9601e97f |
| institution | DOAJ |
| issn | 1594-4077 1828-051X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj-art-e5fe0a43defa44df811ee6fc9601e97f2025-08-20T02:57:53ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2024-12-012311524153410.1080/1828051X.2024.24114132411413Supplementing exogenous xylanase improves the liver antioxidant capacity and immune response of Tibetan sheep fed wheat-based dietsFeng-Shuo Zhang0Bo-Yan Ma1Zhan-Hong Gao2Zhen-Ling Wu3Yang-Ang-Mao Qu4Yu Zhang5Sheng-Zhen Hou6Lin-Sheng Gui7College of Agriculture and Animal Husbandry, Qinghai UniversityCollege of Agriculture and Animal Husbandry, Qinghai UniversityCollege of Agriculture and Animal Husbandry, Qinghai UniversityCollege of Agriculture and Animal Husbandry, Qinghai UniversityCollege of Agriculture and Animal Husbandry, Qinghai UniversityCollege of Agriculture and Animal Husbandry, Qinghai UniversityCollege of Agriculture and Animal Husbandry, Qinghai UniversityCollege of Agriculture and Animal Husbandry, Qinghai UniversityXylanase is a heteropolysaccharide found in the plant cell wall, which aids in the absorption and utilisation of nutrients. When embedded in animal feed, it enhances nutrient availability, leading to improved animal performance. The aim of this study was to explore the effect of xylanase supplementation in wheat-based feeds on the hepatic development, antioxidant capacity, and immune response in Tibetan sheep. Sixty healthy, weaned, non-neutered male lambs (19.35 ± 2.18 kg) were randomly assigned to two equal groups (n = 30 per group). Dietary treatments were: basal diet (H group), and basal diet with 0.2% xylanase supplementation (E group). After a 10–day adaptation period, the feeding trial was carried out for 90 days. The live weight, morphology, antioxidant capacity and immune response were measured. The differential genes in liver were identified by RNA-sequencing. Our results showed that the levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were significantly higher in E group than in H group (p < 0.05). Enzyme-linked immunosorbent assay (ELISA) revealed that immunoglobulin A (IgA), IgM, and IgG were significantly higher in E group than in H group (p < 0.05). The hepatocytes of sheep fed xylanase diet showed arranged radially into hepatic plates cantered around the central vein, and the plates linked with each other to form a lost-like structure. A total of 4,509 differential genes (DEGs) were identified, among which 4,427 were up-regulated and 82 down-regulated. Additionally, the expression of antioxidant-related genes in cluding catalase (CAT), superoxide dismutase 1 (SOD1), oxidation resistance 1 (OXR1) and glutathione peroxidase 2 (GPX2), and immunity-related gene including mitogen-activated protein kinase 1 (MAPK1), signal transducer and activator of transcription 5B (STAT5B), janus kinase 3 (JAK3) and jun proto-oncogene (JUN) were verified using the quantitative reverse transcription polymerase chain reaction (qRT-PCR). Conclusion: In conclusion, dietary 0.2% xylanase supplementation promoted hepatic antioxidant capacity and immune response via modulating the expression of functional genes in Tibetan sheep.http://dx.doi.org/10.1080/1828051X.2024.2411413exogenous enzymehepatic functiontranscriptomeenzyme-linked immunosorbent assayphenotype |
| spellingShingle | Feng-Shuo Zhang Bo-Yan Ma Zhan-Hong Gao Zhen-Ling Wu Yang-Ang-Mao Qu Yu Zhang Sheng-Zhen Hou Lin-Sheng Gui Supplementing exogenous xylanase improves the liver antioxidant capacity and immune response of Tibetan sheep fed wheat-based diets Italian Journal of Animal Science exogenous enzyme hepatic function transcriptome enzyme-linked immunosorbent assay phenotype |
| title | Supplementing exogenous xylanase improves the liver antioxidant capacity and immune response of Tibetan sheep fed wheat-based diets |
| title_full | Supplementing exogenous xylanase improves the liver antioxidant capacity and immune response of Tibetan sheep fed wheat-based diets |
| title_fullStr | Supplementing exogenous xylanase improves the liver antioxidant capacity and immune response of Tibetan sheep fed wheat-based diets |
| title_full_unstemmed | Supplementing exogenous xylanase improves the liver antioxidant capacity and immune response of Tibetan sheep fed wheat-based diets |
| title_short | Supplementing exogenous xylanase improves the liver antioxidant capacity and immune response of Tibetan sheep fed wheat-based diets |
| title_sort | supplementing exogenous xylanase improves the liver antioxidant capacity and immune response of tibetan sheep fed wheat based diets |
| topic | exogenous enzyme hepatic function transcriptome enzyme-linked immunosorbent assay phenotype |
| url | http://dx.doi.org/10.1080/1828051X.2024.2411413 |
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