Improvement of Aspergillus niger for Sodium Gluconate Synthesis by UV Mutation Method
The sodium gluconate synthesis pathway was improved in Aspergillus niger by random mutation method. A. niger was mutated with Ultraviolet (UV) radiation and the alteration of cell bound enzymes activity of gluconic acid synthesis pathway and sodium gluconate synthesis were evaluated. The improved mu...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2012/768901 |
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| author | Rajagopalan Prabu Thomas Chand Sunhare Raksha |
| author_facet | Rajagopalan Prabu Thomas Chand Sunhare Raksha |
| author_sort | Rajagopalan Prabu |
| collection | DOAJ |
| description | The sodium gluconate synthesis pathway was improved in Aspergillus niger by random mutation method. A. niger was mutated with Ultraviolet (UV) radiation and the alteration of cell bound enzymes activity of gluconic acid synthesis pathway and sodium gluconate synthesis were evaluated. The improved mutants (A. niger UV-112) was capable of producing sodium gluconate up to final concentrations of 60 g/L in batch fermentation, which was 3.0 fold higher than the parent strain. It have been observed that the changes in activities of cell bound enzymes related for gluconic acid pathway such as glucose dehydrogenase and glucose oxidase and it were significantly higher than the parent strain. The mutant A. niger strain and the simple method used to decrease the production cost and development of fermentation process for industrial production of gluconic acid or its salt. |
| format | Article |
| id | doaj-art-e5fd945aafe5447db66b420fe7496fab |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-e5fd945aafe5447db66b420fe7496fab2025-02-03T01:10:59ZengWileyE-Journal of Chemistry0973-49452090-98102012-01-01942052205710.1155/2012/768901Improvement of Aspergillus niger for Sodium Gluconate Synthesis by UV Mutation MethodRajagopalan Prabu0Thomas Chand1Sunhare Raksha2Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaDepartment of Bioscience, Christian Eminent Academy, Indore 452001, IndiaInstitute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaThe sodium gluconate synthesis pathway was improved in Aspergillus niger by random mutation method. A. niger was mutated with Ultraviolet (UV) radiation and the alteration of cell bound enzymes activity of gluconic acid synthesis pathway and sodium gluconate synthesis were evaluated. The improved mutants (A. niger UV-112) was capable of producing sodium gluconate up to final concentrations of 60 g/L in batch fermentation, which was 3.0 fold higher than the parent strain. It have been observed that the changes in activities of cell bound enzymes related for gluconic acid pathway such as glucose dehydrogenase and glucose oxidase and it were significantly higher than the parent strain. The mutant A. niger strain and the simple method used to decrease the production cost and development of fermentation process for industrial production of gluconic acid or its salt.http://dx.doi.org/10.1155/2012/768901 |
| spellingShingle | Rajagopalan Prabu Thomas Chand Sunhare Raksha Improvement of Aspergillus niger for Sodium Gluconate Synthesis by UV Mutation Method E-Journal of Chemistry |
| title | Improvement of Aspergillus niger for Sodium Gluconate Synthesis by UV Mutation Method |
| title_full | Improvement of Aspergillus niger for Sodium Gluconate Synthesis by UV Mutation Method |
| title_fullStr | Improvement of Aspergillus niger for Sodium Gluconate Synthesis by UV Mutation Method |
| title_full_unstemmed | Improvement of Aspergillus niger for Sodium Gluconate Synthesis by UV Mutation Method |
| title_short | Improvement of Aspergillus niger for Sodium Gluconate Synthesis by UV Mutation Method |
| title_sort | improvement of aspergillus niger for sodium gluconate synthesis by uv mutation method |
| url | http://dx.doi.org/10.1155/2012/768901 |
| work_keys_str_mv | AT rajagopalanprabu improvementofaspergillusnigerforsodiumgluconatesynthesisbyuvmutationmethod AT thomaschand improvementofaspergillusnigerforsodiumgluconatesynthesisbyuvmutationmethod AT sunhareraksha improvementofaspergillusnigerforsodiumgluconatesynthesisbyuvmutationmethod |