Novel Strain <i>Bacillus velezensis</i> LAFUEL 03: Activity Against <i>Xanthomonas vasicola</i> pv. <i>vasculorum</i>, Control of Bacterial Leaf Streak of Corn and Genome Insights into Its Antagonistic Activity

Novel Strain <i>Bacillus velezensis</i> LAFUEL 03: Activity Against <i>Xanthomonas vasicola</i> pv. <i>vasculorum</i>, Control of Bacterial Leaf Streak of Corn and Genome Insights into Its Antagonistic Activity

The main objective of this study was to investigate the antimicrobial activity of three putative antagonist bacterial strains of <i>Bacillus</i> spp. against <i>Xanthomonas vasicola</i> pv. <i>vasculorum</i> (<i>Xvv</i>) and their potential to control...

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Bibliographic Details
Main Authors:	Izabela Moura Duin, Vanessa Hitomi Sugahara Rodrigues, Rui Pereira Leite, Maria Isabel Balbi-Peña
Format:	Article
Language:	English
Published:	MDPI AG 2024-10-01
Series:	Agronomy
Subjects:	antimicrobial activity <i>Bacillus velezensis</i> bacterial genomics biological control biosynthetic gene clusters molecular identification
Online Access:	https://www.mdpi.com/2073-4395/14/11/2495
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Summary:	The main objective of this study was to investigate the antimicrobial activity of three putative antagonist bacterial strains of <i>Bacillus</i> spp. against <i>Xanthomonas vasicola</i> pv. <i>vasculorum</i> (<i>Xvv</i>) and their potential to control bacterial leaf streak (BLS) of corn. Additionally, the study included investigations on the genome of one of these antagonist bacteria, such as genome sequencing and mining of genes involved in biofilm formation, swarming motility, and synthesis of secondary metabolites. The growth of <i>Xvv</i> was inhibited by both cell suspensions and cell-free supernatants of the bacterial strains LAFUEL 01, LAFUEL 02, and LAFUEL 03 in agar diffusion tests. All three antagonist strains significantly reduced the severity of BLS in the 3rd and 4th leaves of corn plants that were artificially inoculated at the V3 growth stage under greenhouse conditions. The 16S <i>rRNA</i> sequencing confirmed that the antagonistic bacterial strains belong to the genus <i>Bacillus</i>, with LAFUEL 03 having approximately 97% similarity to <i>B. velezensis</i>. <i>B. velezensis</i> LAFUEL 03 harbors genes related to the biosynthesis of secondary metabolites, biofilm formation/regulation, and swarming motility that enhances its potential for controlling BLS in corn and suggests a promising candidate for the development of a commercial biocontrol agent.
ISSN:	2073-4395