Silent Human Trypanosoma brucei gambiense Infections around the Old Gboko Sleeping Sickness Focus in Nigeria
Trypanosoma brucei gambiense causes Gambian trypanosomosis, a disease ravaging affected rural parts of Sub-Saharan Africa. We screened 1200 human blood samples for T. b. gambiense using the card agglutination test for trypanosomosis, characterized trypanosome isolates with Trypanosoma gambiense seru...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/2656121 |
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| author | Karshima Solomon Ngutor Lawal A. Idris Okubanjo Oluseyi Oluyinka |
| author_facet | Karshima Solomon Ngutor Lawal A. Idris Okubanjo Oluseyi Oluyinka |
| author_sort | Karshima Solomon Ngutor |
| collection | DOAJ |
| description | Trypanosoma brucei gambiense causes Gambian trypanosomosis, a disease ravaging affected rural parts of Sub-Saharan Africa. We screened 1200 human blood samples for T. b. gambiense using the card agglutination test for trypanosomosis, characterized trypanosome isolates with Trypanosoma gambiense serum glycoprotein-PCR (TgsGP-PCR), and analyzed our data using Chi square and odds ratio at 95% confidence interval for statistical association. Of the 1200 samples, the CATT revealed an overall infection rate of 1.8% which ranged between 0.0% and 3.5% across study sites. Age and sex based infection rates ranged between 1.2% and 2.3%. We isolated 7 (33.3%) trypanosomes from the 21 seropositive samples using immunosuppressed mice which were identified as T. b. gambiense group 1 by TgsGP-PCR. Based on study sites, PCR revealed an overall infection rate of 0.6% which ranged between 0.0% and 1.5%. Females and males revealed PCR based infection rates of 0.3% and 0.8%, respectively. Infection rates in adults (1.3%) and children (0.1%) varied significantly (p<0.05). We observed silent T. b. gambiense infections among residents of this focus. Risks of disease development into the second fatal stage in these patients who may also serve as reservoirs of infection in the focus exist. |
| format | Article |
| id | doaj-art-e5ebab987b8140f283776d385a2c4bc1 |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-e5ebab987b8140f283776d385a2c4bc12025-02-03T01:11:44ZengWileyJournal of Parasitology Research2090-00232090-00312016-01-01201610.1155/2016/26561212656121Silent Human Trypanosoma brucei gambiense Infections around the Old Gboko Sleeping Sickness Focus in NigeriaKarshima Solomon Ngutor0Lawal A. Idris1Okubanjo Oluseyi Oluyinka2Department of Animal Health, Federal College of Animal Health and Production Technology, PMB 001, Vom, NigeriaDepartment of Veterinary Parasitology and Entomology, Ahmadu Bello University, PMB 1045, Zaria, NigeriaDepartment of Veterinary Parasitology and Entomology, Ahmadu Bello University, PMB 1045, Zaria, NigeriaTrypanosoma brucei gambiense causes Gambian trypanosomosis, a disease ravaging affected rural parts of Sub-Saharan Africa. We screened 1200 human blood samples for T. b. gambiense using the card agglutination test for trypanosomosis, characterized trypanosome isolates with Trypanosoma gambiense serum glycoprotein-PCR (TgsGP-PCR), and analyzed our data using Chi square and odds ratio at 95% confidence interval for statistical association. Of the 1200 samples, the CATT revealed an overall infection rate of 1.8% which ranged between 0.0% and 3.5% across study sites. Age and sex based infection rates ranged between 1.2% and 2.3%. We isolated 7 (33.3%) trypanosomes from the 21 seropositive samples using immunosuppressed mice which were identified as T. b. gambiense group 1 by TgsGP-PCR. Based on study sites, PCR revealed an overall infection rate of 0.6% which ranged between 0.0% and 1.5%. Females and males revealed PCR based infection rates of 0.3% and 0.8%, respectively. Infection rates in adults (1.3%) and children (0.1%) varied significantly (p<0.05). We observed silent T. b. gambiense infections among residents of this focus. Risks of disease development into the second fatal stage in these patients who may also serve as reservoirs of infection in the focus exist.http://dx.doi.org/10.1155/2016/2656121 |
| spellingShingle | Karshima Solomon Ngutor Lawal A. Idris Okubanjo Oluseyi Oluyinka Silent Human Trypanosoma brucei gambiense Infections around the Old Gboko Sleeping Sickness Focus in Nigeria Journal of Parasitology Research |
| title | Silent Human Trypanosoma brucei gambiense Infections around the Old Gboko Sleeping Sickness Focus in Nigeria |
| title_full | Silent Human Trypanosoma brucei gambiense Infections around the Old Gboko Sleeping Sickness Focus in Nigeria |
| title_fullStr | Silent Human Trypanosoma brucei gambiense Infections around the Old Gboko Sleeping Sickness Focus in Nigeria |
| title_full_unstemmed | Silent Human Trypanosoma brucei gambiense Infections around the Old Gboko Sleeping Sickness Focus in Nigeria |
| title_short | Silent Human Trypanosoma brucei gambiense Infections around the Old Gboko Sleeping Sickness Focus in Nigeria |
| title_sort | silent human trypanosoma brucei gambiense infections around the old gboko sleeping sickness focus in nigeria |
| url | http://dx.doi.org/10.1155/2016/2656121 |
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