Blood Coagulation Favors Anti-Inflammatory Immune Responses in Whole Blood
Background: We studied the effects of human blood coagulation on antioxidant activity and the cellular secretion of immunoregulatory molecules in vitro. Methods: Reactive oxygen species (ROS) activity and cytokine content were determined in plasma and serum blood samples incubated with lipopolysacch...
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| Language: | English |
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MDPI AG
2025-04-01
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| Series: | Hematology Reports |
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| Online Access: | https://www.mdpi.com/2038-8330/17/2/19 |
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| author | Victor I. Seledtsov Anatoly A. Pyshenko Tatyana Ya. Lyubavskaya Irina A. Seledtsova Alexei A. von Delwig |
| author_facet | Victor I. Seledtsov Anatoly A. Pyshenko Tatyana Ya. Lyubavskaya Irina A. Seledtsova Alexei A. von Delwig |
| author_sort | Victor I. Seledtsov |
| collection | DOAJ |
| description | Background: We studied the effects of human blood coagulation on antioxidant activity and the cellular secretion of immunoregulatory molecules in vitro. Methods: Reactive oxygen species (ROS) activity and cytokine content were determined in plasma and serum blood samples incubated with lipopolysaccharide (LPS) for 3 h or 18 h. Results: Coagulation process significantly decreased ROS activity induced by LPS in blood samples from healthy donors. Human serum was found to have significantly higher antioxidant activity than plasma. Blood coagulation markedly reduced LPS-induced secretion of TNF-α by cells, without significantly affecting the secretion of interleukin-1 (IL-1), IL-6, IL-8, or C-reactive protein (CRP). Blood clotting led to an increase in LPS-induced release of vascular endothelial growth factor (VEGF) by blood cells. A significant increase in procalcitonin levels was also observed in serum samples. Conclusions: Blood clotting enhances the antioxidant and anti-inflammatory functions of immunoreactive blood cells. |
| format | Article |
| id | doaj-art-e5de3aa6396f48a38f1e5ca6ad719c5f |
| institution | OA Journals |
| issn | 2038-8330 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Hematology Reports |
| spelling | doaj-art-e5de3aa6396f48a38f1e5ca6ad719c5f2025-08-20T02:28:36ZengMDPI AGHematology Reports2038-83302025-04-011721910.3390/hematolrep17020019Blood Coagulation Favors Anti-Inflammatory Immune Responses in Whole BloodVictor I. Seledtsov0Anatoly A. Pyshenko1Tatyana Ya. Lyubavskaya2Irina A. Seledtsova3Alexei A. von Delwig4Federal State Budgetary Scientific Institution, Russian Scientific Center for Surgery Named after Academician B.V. Petrovsky, 119991 Moscow, RussiaFederal State Budgetary Scientific Institution, Russian Scientific Center for Surgery Named after Academician B.V. Petrovsky, 119991 Moscow, RussiaFederal State Budgetary Scientific Institution, Russian Scientific Center for Surgery Named after Academician B.V. Petrovsky, 119991 Moscow, RussiaFederal State Budgetary Scientific Institution, Russian Scientific Center for Surgery Named after Academician B.V. Petrovsky, 119991 Moscow, RussiaFederal State Budgetary Scientific Institution, Russian Scientific Center for Surgery Named after Academician B.V. Petrovsky, 119991 Moscow, RussiaBackground: We studied the effects of human blood coagulation on antioxidant activity and the cellular secretion of immunoregulatory molecules in vitro. Methods: Reactive oxygen species (ROS) activity and cytokine content were determined in plasma and serum blood samples incubated with lipopolysaccharide (LPS) for 3 h or 18 h. Results: Coagulation process significantly decreased ROS activity induced by LPS in blood samples from healthy donors. Human serum was found to have significantly higher antioxidant activity than plasma. Blood coagulation markedly reduced LPS-induced secretion of TNF-α by cells, without significantly affecting the secretion of interleukin-1 (IL-1), IL-6, IL-8, or C-reactive protein (CRP). Blood clotting led to an increase in LPS-induced release of vascular endothelial growth factor (VEGF) by blood cells. A significant increase in procalcitonin levels was also observed in serum samples. Conclusions: Blood clotting enhances the antioxidant and anti-inflammatory functions of immunoreactive blood cells.https://www.mdpi.com/2038-8330/17/2/19blood coagulationwhole blood immunoreactivityreactive oxygen speciescytokinesregeneration |
| spellingShingle | Victor I. Seledtsov Anatoly A. Pyshenko Tatyana Ya. Lyubavskaya Irina A. Seledtsova Alexei A. von Delwig Blood Coagulation Favors Anti-Inflammatory Immune Responses in Whole Blood Hematology Reports blood coagulation whole blood immunoreactivity reactive oxygen species cytokines regeneration |
| title | Blood Coagulation Favors Anti-Inflammatory Immune Responses in Whole Blood |
| title_full | Blood Coagulation Favors Anti-Inflammatory Immune Responses in Whole Blood |
| title_fullStr | Blood Coagulation Favors Anti-Inflammatory Immune Responses in Whole Blood |
| title_full_unstemmed | Blood Coagulation Favors Anti-Inflammatory Immune Responses in Whole Blood |
| title_short | Blood Coagulation Favors Anti-Inflammatory Immune Responses in Whole Blood |
| title_sort | blood coagulation favors anti inflammatory immune responses in whole blood |
| topic | blood coagulation whole blood immunoreactivity reactive oxygen species cytokines regeneration |
| url | https://www.mdpi.com/2038-8330/17/2/19 |
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