T-cell immune status in patients with acute exacerbation of chronic obstructive pulmonary disease: a case-control study
IntroductionImmune inflammatory response plays an important role in chronic obstructive pulmonary disease (COPD). However, the cellular immune status of patients with COPD at different phases is unclear. Herein, we aim to investigate the distribution and functional status of T cell subsets in differ...
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Frontiers Media S.A.
2025-01-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2025.1433844/full |
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| author | Xiao-feng Xiong Xiao-feng Xiong Min Zhu Min Zhu Hong-xia Wu Zuo-hong Wu Li-li Fan De-yun Cheng |
| author_facet | Xiao-feng Xiong Xiao-feng Xiong Min Zhu Min Zhu Hong-xia Wu Zuo-hong Wu Li-li Fan De-yun Cheng |
| author_sort | Xiao-feng Xiong |
| collection | DOAJ |
| description | IntroductionImmune inflammatory response plays an important role in chronic obstructive pulmonary disease (COPD). However, the cellular immune status of patients with COPD at different phases is unclear. Herein, we aim to investigate the distribution and functional status of T cell subsets in different phases of COPD (acute exacerbation of COPD [AECOPD] and stable COPD [SCOPD]).MethodsThis is an observational case-control study undertaken in West China Hospital. The distribution of T cell subsets in peripheral blood of AECOPD, SCOPD, and healthy controls (HCs) was measured using multi-color flow cytometry, and the functional status was analyzed by additional staining of activation markers.ResultsA total of 43 HCs, 43 SCOPD patients, and 64 AECOPD patients were evaluated. The total number and percentage of lymphocytes and the CD4+/CD8+ T cells ratio were significantly lower in AECOPD patients when compared to HCs. HLA-DR expression in CD3+, CD4+, CD8+, CD8+ TCR aβ, and CD4+ TCR aβ T cells was upregulated in the AECOPD group. Similarly, the expressions of HLA-DR, CD57, and PD-1 were higher in T cell subsets in the AECOPD group. Compared with the SCOPD and HC groups, the AECOPD had a significantly lower proportion of CD4+CD27+CD28+ T cells, but opposite results were found for CD4+CD27-CD28- T cells. In addition, the proportion of CD4+CD39+ T cells and CD4+CD25+FoxP3+ T cells was significantly higher in the AECOPD and SCOPD groups when compared to the HC group (P < 0.05).DiscussionThe distribution of nearly half the T cell subsets in AECOPD patients was significantly different from that in SCOPD patients and HCs. AECOPD patients may have cellular immune suppression, immune dysfunction, abnormal activation, and higher senescence depletion of T cells. |
| format | Article |
| id | doaj-art-e5d499a2540c49bfa4d7540928819618 |
| institution | Kabale University |
| issn | 2296-858X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Medicine |
| spelling | doaj-art-e5d499a2540c49bfa4d75409288196182025-01-24T05:21:19ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2025-01-011210.3389/fmed.2025.14338441433844T-cell immune status in patients with acute exacerbation of chronic obstructive pulmonary disease: a case-control studyXiao-feng Xiong0Xiao-feng Xiong1Min Zhu2Min Zhu3Hong-xia Wu4Zuo-hong Wu5Li-li Fan6De-yun Cheng7Department of Respiratory and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu, ChinaLaboratory of Pulmonary Immunology and Inflammation, Frontiers Science Center for Disease-Related Molecular Network, Sichuan University, Chengdu, ChinaDepartment of Respiratory and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu, ChinaLaboratory of Pulmonary Immunology and Inflammation, Frontiers Science Center for Disease-Related Molecular Network, Sichuan University, Chengdu, ChinaDepartment of Respiratory and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Respiratory and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Respiratory and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Respiratory and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu, ChinaIntroductionImmune inflammatory response plays an important role in chronic obstructive pulmonary disease (COPD). However, the cellular immune status of patients with COPD at different phases is unclear. Herein, we aim to investigate the distribution and functional status of T cell subsets in different phases of COPD (acute exacerbation of COPD [AECOPD] and stable COPD [SCOPD]).MethodsThis is an observational case-control study undertaken in West China Hospital. The distribution of T cell subsets in peripheral blood of AECOPD, SCOPD, and healthy controls (HCs) was measured using multi-color flow cytometry, and the functional status was analyzed by additional staining of activation markers.ResultsA total of 43 HCs, 43 SCOPD patients, and 64 AECOPD patients were evaluated. The total number and percentage of lymphocytes and the CD4+/CD8+ T cells ratio were significantly lower in AECOPD patients when compared to HCs. HLA-DR expression in CD3+, CD4+, CD8+, CD8+ TCR aβ, and CD4+ TCR aβ T cells was upregulated in the AECOPD group. Similarly, the expressions of HLA-DR, CD57, and PD-1 were higher in T cell subsets in the AECOPD group. Compared with the SCOPD and HC groups, the AECOPD had a significantly lower proportion of CD4+CD27+CD28+ T cells, but opposite results were found for CD4+CD27-CD28- T cells. In addition, the proportion of CD4+CD39+ T cells and CD4+CD25+FoxP3+ T cells was significantly higher in the AECOPD and SCOPD groups when compared to the HC group (P < 0.05).DiscussionThe distribution of nearly half the T cell subsets in AECOPD patients was significantly different from that in SCOPD patients and HCs. AECOPD patients may have cellular immune suppression, immune dysfunction, abnormal activation, and higher senescence depletion of T cells.https://www.frontiersin.org/articles/10.3389/fmed.2025.1433844/fullchronic obstructive pulmonary diseaseacute exacerbationT cellsimmunitymulti-color flow cytometry |
| spellingShingle | Xiao-feng Xiong Xiao-feng Xiong Min Zhu Min Zhu Hong-xia Wu Zuo-hong Wu Li-li Fan De-yun Cheng T-cell immune status in patients with acute exacerbation of chronic obstructive pulmonary disease: a case-control study Frontiers in Medicine chronic obstructive pulmonary disease acute exacerbation T cells immunity multi-color flow cytometry |
| title | T-cell immune status in patients with acute exacerbation of chronic obstructive pulmonary disease: a case-control study |
| title_full | T-cell immune status in patients with acute exacerbation of chronic obstructive pulmonary disease: a case-control study |
| title_fullStr | T-cell immune status in patients with acute exacerbation of chronic obstructive pulmonary disease: a case-control study |
| title_full_unstemmed | T-cell immune status in patients with acute exacerbation of chronic obstructive pulmonary disease: a case-control study |
| title_short | T-cell immune status in patients with acute exacerbation of chronic obstructive pulmonary disease: a case-control study |
| title_sort | t cell immune status in patients with acute exacerbation of chronic obstructive pulmonary disease a case control study |
| topic | chronic obstructive pulmonary disease acute exacerbation T cells immunity multi-color flow cytometry |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2025.1433844/full |
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