Cytotoxic Effect of the Genus Sinularia Extracts on Human SCC25 and HaCaT Cells
Soft corals of the genus Sinularia are being increasingly adopted to treat a wide variety of disease processes. However, the mechanism underlying its activity against human oral cancer cells is poorly understood. This study evaluates the cyototoxicity effects of the genus Sinularia extracts (S. gra...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2009/634868 |
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| author | Guey-Horng Wang Tzung-Han Chou Rong-Jyh Lin Jyh-Horng Sheu Shih-Hao Wang Chia-Hua Liang |
| author_facet | Guey-Horng Wang Tzung-Han Chou Rong-Jyh Lin Jyh-Horng Sheu Shih-Hao Wang Chia-Hua Liang |
| author_sort | Guey-Horng Wang |
| collection | DOAJ |
| description | Soft corals of the genus Sinularia are being increasingly adopted to treat a wide variety of disease processes. However, the mechanism underlying its activity against human oral cancer cells is poorly understood. This study evaluates the cyototoxicity effects of the genus Sinularia extracts (S. grandilobata, S. parva, S. triangula, S. scabra, S. nanolobata and S. gibberosa) by SCC25 and HaCaT cells. The cell adhesion assay indicates that extracts reduce the cell attachment. Extracts exhibit a dose-dependent cytotoxic effect using MTS assay.Treatment of extracts to observe the morphological alterations in cells, membrane blebbing, nuclear condensation, and apoptotic bodies is demonstrated. Flow cytometry shows that extracts sensitized the cells in the G0/G1 and G2/M phases with a concomitant significantly increased sub-G1 fraction, suggesting cell death by apoptosis. Extracts of the genus Sinularia thus apparently cause apoptosis of SCC25 and HaCaT cells, and warrant further research investigating the possible antioral cancer compounds in these soft corals. |
| format | Article |
| id | doaj-art-e5c579e0aae0422b89ca776e1bcb4d9d |
| institution | Kabale University |
| issn | 1687-8191 1687-8205 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-e5c579e0aae0422b89ca776e1bcb4d9d2025-02-03T01:24:34ZengWileyJournal of Toxicology1687-81911687-82052009-01-01200910.1155/2009/634868634868Cytotoxic Effect of the Genus Sinularia Extracts on Human SCC25 and HaCaT CellsGuey-Horng Wang0Tzung-Han Chou1Rong-Jyh Lin2Jyh-Horng Sheu3Shih-Hao Wang4Chia-Hua Liang5Department of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan 717, TaiwanDepartment of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan 717, TaiwanDepartment of Parasitology, Faculty of Medicine, Kaohsiung Medical University, Kaohsiung 807, TaiwanDepartment of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung 804, TaiwanGraduate Institute of Pharmaceutical Chemistry, China Medical University, Taichung 404, TaiwanDepartment of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan 717, TaiwanSoft corals of the genus Sinularia are being increasingly adopted to treat a wide variety of disease processes. However, the mechanism underlying its activity against human oral cancer cells is poorly understood. This study evaluates the cyototoxicity effects of the genus Sinularia extracts (S. grandilobata, S. parva, S. triangula, S. scabra, S. nanolobata and S. gibberosa) by SCC25 and HaCaT cells. The cell adhesion assay indicates that extracts reduce the cell attachment. Extracts exhibit a dose-dependent cytotoxic effect using MTS assay.Treatment of extracts to observe the morphological alterations in cells, membrane blebbing, nuclear condensation, and apoptotic bodies is demonstrated. Flow cytometry shows that extracts sensitized the cells in the G0/G1 and G2/M phases with a concomitant significantly increased sub-G1 fraction, suggesting cell death by apoptosis. Extracts of the genus Sinularia thus apparently cause apoptosis of SCC25 and HaCaT cells, and warrant further research investigating the possible antioral cancer compounds in these soft corals.http://dx.doi.org/10.1155/2009/634868 |
| spellingShingle | Guey-Horng Wang Tzung-Han Chou Rong-Jyh Lin Jyh-Horng Sheu Shih-Hao Wang Chia-Hua Liang Cytotoxic Effect of the Genus Sinularia Extracts on Human SCC25 and HaCaT Cells Journal of Toxicology |
| title | Cytotoxic Effect of the Genus Sinularia Extracts on Human SCC25 and HaCaT Cells |
| title_full | Cytotoxic Effect of the Genus Sinularia Extracts on Human SCC25 and HaCaT Cells |
| title_fullStr | Cytotoxic Effect of the Genus Sinularia Extracts on Human SCC25 and HaCaT Cells |
| title_full_unstemmed | Cytotoxic Effect of the Genus Sinularia Extracts on Human SCC25 and HaCaT Cells |
| title_short | Cytotoxic Effect of the Genus Sinularia Extracts on Human SCC25 and HaCaT Cells |
| title_sort | cytotoxic effect of the genus sinularia extracts on human scc25 and hacat cells |
| url | http://dx.doi.org/10.1155/2009/634868 |
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