Choosing the most suitable NGS technology to combine with a standardized viral enrichment protocol for obtaining complete avian orthoreovirus genomes from metagenomic samples

Choosing the most suitable NGS technology to combine with a standardized viral enrichment protocol for obtaining complete avian orthoreovirus genomes from metagenomic samples

Since viruses are obligate intracellular pathogens, sequencing their genomes results in metagenomic data from both the virus and the host. Virology researchers are constantly seeking new, cost-effective strategies and bioinformatic pipelines for the retrieval of complete viral genomes from these met...

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Bibliographic Details
Main Authors: Sonsiray Álvarez-Narváez, Telvin L. Harrell, Islam Nour, Sujit K. Mohanty, Steven J. Conrad
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-02-01
Series:Frontiers in Bioinformatics
Subjects:
avian orthoreovirus
ARV
whole genome sequencing
WGS
short-read sequencing
long-read sequencing
Online Access:https://www.frontiersin.org/articles/10.3389/fbinf.2025.1498921/full
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