Effect of Pro12Ala polymorphism of PPARG gene on lipid peroxidation and antioxidant defense in patients with type 2 diabetes depending on the duration of the disease
Aim – to study the impact of polymorphic marker rs1801282 of gene PPARG on the formation of oxidative stress in terms of LPO and AOS in patients with type 2 diabetes depending on the duration of the disease. Materials and methods. The study was conducted involving 138 patients, 88 of whom were pa...
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Zaporizhzhia State Medical and Pharmaceutical University
2016-08-01
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| Online Access: | http://pat.zsmu.edu.ua/article/view/81332/78226 |
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| author | V. Y. Mokrii S. V. Ziablytsev |
| author_facet | V. Y. Mokrii S. V. Ziablytsev |
| author_sort | V. Y. Mokrii |
| collection | DOAJ |
| description | Aim – to study the impact of polymorphic marker rs1801282 of gene PPARG on the formation of oxidative stress in terms of LPO and AOS in patients with type 2 diabetes depending on the duration of the disease.
Materials and methods. The study was conducted involving 138 patients, 88 of whom were patients with type 2 diabetes and the control group consisted of 50 people. Patients were divided into three groups according to disease duration: 5 years, 5-10 years and more than 10 years. LPO activity was assessed in terms of diene conjugates (DC) and malonic dialdehyde (MDA) and AOC status - the activity of superoxide dismutase (SOD), catalase and the level of α-tocopherol (α-TF). Molecular genetic studies were conducted by the method of PCR in real time, and to analyze polymorphic DNA loci a standardized test system TaqMan Mutation Detection Assays Life-Technology (USA) was used.
Results. During 5-10 years disease levels of DC and MDA in patients with Pro12Ala genotype were increased by 34,9% and 34,7%, as compared to Pro12Pro (p=0,01). Availability of Pro12Pro stipulated the reduction of catalase activity during 5-10 years disease by 75% (p=0,001), and for those, who are ill for more than 10 years by 2,04 times (p=0,01), which is not different from the reference level (F=1,19; p=0,600), but in the case of Pro12Ala, this figure was 2 times higher.
Conclusions. Pro12Ala polymorphism (rs1801282) of PPARG gene causes the development of oxidative stress in type 2 diabetes with 5-10 years durations, and genotype Pro12Pro - lack of the catalase enzyme level of antioxidant system in patients with durations of disease more than 5 years. |
| format | Article |
| id | doaj-art-e555fea036e0474a981a1cd1e1ffb2c8 |
| institution | OA Journals |
| issn | 2306-8027 2310-1237 |
| language | English |
| publishDate | 2016-08-01 |
| publisher | Zaporizhzhia State Medical and Pharmaceutical University |
| record_format | Article |
| series | Patologìâ |
| spelling | doaj-art-e555fea036e0474a981a1cd1e1ffb2c82025-08-20T02:04:30ZengZaporizhzhia State Medical and Pharmaceutical UniversityPatologìâ2306-80272310-12372016-08-012525710.14739/2310-1237.2016.2.81332Effect of Pro12Ala polymorphism of PPARG gene on lipid peroxidation and antioxidant defense in patients with type 2 diabetes depending on the duration of the diseaseV. Y. MokriiS. V. ZiablytsevAim – to study the impact of polymorphic marker rs1801282 of gene PPARG on the formation of oxidative stress in terms of LPO and AOS in patients with type 2 diabetes depending on the duration of the disease. Materials and methods. The study was conducted involving 138 patients, 88 of whom were patients with type 2 diabetes and the control group consisted of 50 people. Patients were divided into three groups according to disease duration: 5 years, 5-10 years and more than 10 years. LPO activity was assessed in terms of diene conjugates (DC) and malonic dialdehyde (MDA) and AOC status - the activity of superoxide dismutase (SOD), catalase and the level of α-tocopherol (α-TF). Molecular genetic studies were conducted by the method of PCR in real time, and to analyze polymorphic DNA loci a standardized test system TaqMan Mutation Detection Assays Life-Technology (USA) was used. Results. During 5-10 years disease levels of DC and MDA in patients with Pro12Ala genotype were increased by 34,9% and 34,7%, as compared to Pro12Pro (p=0,01). Availability of Pro12Pro stipulated the reduction of catalase activity during 5-10 years disease by 75% (p=0,001), and for those, who are ill for more than 10 years by 2,04 times (p=0,01), which is not different from the reference level (F=1,19; p=0,600), but in the case of Pro12Ala, this figure was 2 times higher. Conclusions. Pro12Ala polymorphism (rs1801282) of PPARG gene causes the development of oxidative stress in type 2 diabetes with 5-10 years durations, and genotype Pro12Pro - lack of the catalase enzyme level of antioxidant system in patients with durations of disease more than 5 years.http://pat.zsmu.edu.ua/article/view/81332/78226Pro12Ala polymorphism of PPARG genetype 2 diabetesoxidative stress |
| spellingShingle | V. Y. Mokrii S. V. Ziablytsev Effect of Pro12Ala polymorphism of PPARG gene on lipid peroxidation and antioxidant defense in patients with type 2 diabetes depending on the duration of the disease Patologìâ Pro12Ala polymorphism of PPARG gene type 2 diabetes oxidative stress |
| title | Effect of Pro12Ala polymorphism of PPARG gene on lipid peroxidation and antioxidant defense in patients with type 2 diabetes depending on the duration of the disease |
| title_full | Effect of Pro12Ala polymorphism of PPARG gene on lipid peroxidation and antioxidant defense in patients with type 2 diabetes depending on the duration of the disease |
| title_fullStr | Effect of Pro12Ala polymorphism of PPARG gene on lipid peroxidation and antioxidant defense in patients with type 2 diabetes depending on the duration of the disease |
| title_full_unstemmed | Effect of Pro12Ala polymorphism of PPARG gene on lipid peroxidation and antioxidant defense in patients with type 2 diabetes depending on the duration of the disease |
| title_short | Effect of Pro12Ala polymorphism of PPARG gene on lipid peroxidation and antioxidant defense in patients with type 2 diabetes depending on the duration of the disease |
| title_sort | effect of pro12ala polymorphism of pparg gene on lipid peroxidation and antioxidant defense in patients with type 2 diabetes depending on the duration of the disease |
| topic | Pro12Ala polymorphism of PPARG gene type 2 diabetes oxidative stress |
| url | http://pat.zsmu.edu.ua/article/view/81332/78226 |
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