Pharmaco-Toxicological Effects of <i>Cachrys libanotis</i> Extract: Antioxidant, Antimicrobial, and Cytotoxic Activities in Human Cell Lines and Embryonic Models

We conducted a study to explore the potential of an enriched coumarin extract from <i>Cachrys libanotis</i> for the prevention and treatment of various diseases. The extract was prepared using pressurized cyclic solid–liquid extraction, and its safety profile was thoroughly evaluated usi...

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Main Authors: Mary Fucile, Ioana Gabriela Macasoi, Monica Negrea, Diana Obistioiu, Mariangela Marrelli, Ersilia Alexa, Cristina Dehelean, Giancarlo Statti, Filomena Conforti, Iulia Pinzaru
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Antioxidants
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Online Access:https://www.mdpi.com/2076-3921/14/7/810
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author Mary Fucile
Ioana Gabriela Macasoi
Monica Negrea
Diana Obistioiu
Mariangela Marrelli
Ersilia Alexa
Cristina Dehelean
Giancarlo Statti
Filomena Conforti
Iulia Pinzaru
author_facet Mary Fucile
Ioana Gabriela Macasoi
Monica Negrea
Diana Obistioiu
Mariangela Marrelli
Ersilia Alexa
Cristina Dehelean
Giancarlo Statti
Filomena Conforti
Iulia Pinzaru
author_sort Mary Fucile
collection DOAJ
description We conducted a study to explore the potential of an enriched coumarin extract from <i>Cachrys libanotis</i> for the prevention and treatment of various diseases. The extract was prepared using pressurized cyclic solid–liquid extraction, and its safety profile was thoroughly evaluated using both cellular and embryonic models. Our main goal was to uncover a mixture of bioactive compounds that could offer therapeutic benefits. The following parameters were assessed: (i) extract composition; (ii) antioxidant activity; (iii) effects on cell viability and morphology; (iv) irritant potential (in ovo); and (v) antimicrobial activity against nine microbial strains. Chromatographic and spectrometric analyses confirmed that the main specialized metabolites in <i>C. libanotis</i> extract were furanocoumarins, with xanthotoxin, bergapten, and isopimpinellin identified as the three predominant constituents. Treatment with the <i>C. libanotis</i> extract did not induce significant alterations in the adherent human keratinocytes, with confluence and epithelial morphology comparable to control cells. Conversely, viable cells declined in the breast carcinoma cell line (MDA-MB-231). Moreover, the <i>C. libanotis</i> extract exhibited a promising antimicrobial activity against two Gram-negative pathogens, <i>Shigella flexneri</i> and <i>Salmonella typhimurium</i>.
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spelling doaj-art-e550c19031184eb4abdebf317ca60d752025-08-20T03:55:48ZengMDPI AGAntioxidants2076-39212025-06-0114781010.3390/antiox14070810Pharmaco-Toxicological Effects of <i>Cachrys libanotis</i> Extract: Antioxidant, Antimicrobial, and Cytotoxic Activities in Human Cell Lines and Embryonic ModelsMary Fucile0Ioana Gabriela Macasoi1Monica Negrea2Diana Obistioiu3Mariangela Marrelli4Ersilia Alexa5Cristina Dehelean6Giancarlo Statti7Filomena Conforti8Iulia Pinzaru9Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, Cosenza, ItalyFaculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, RomaniaFaculty of Food Engineering, University of Life Sciences “King Mihai I”, Timisoara Aradului Street No.119, 300645 Timisoara, RomaniaFaculty of Food Engineering, University of Life Sciences “King Mihai I”, Timisoara Aradului Street No.119, 300645 Timisoara, RomaniaDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, Cosenza, ItalyFaculty of Food Engineering, University of Life Sciences “King Mihai I”, Timisoara Aradului Street No.119, 300645 Timisoara, RomaniaFaculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, RomaniaDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, Cosenza, ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, Cosenza, ItalyFaculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, RomaniaWe conducted a study to explore the potential of an enriched coumarin extract from <i>Cachrys libanotis</i> for the prevention and treatment of various diseases. The extract was prepared using pressurized cyclic solid–liquid extraction, and its safety profile was thoroughly evaluated using both cellular and embryonic models. Our main goal was to uncover a mixture of bioactive compounds that could offer therapeutic benefits. The following parameters were assessed: (i) extract composition; (ii) antioxidant activity; (iii) effects on cell viability and morphology; (iv) irritant potential (in ovo); and (v) antimicrobial activity against nine microbial strains. Chromatographic and spectrometric analyses confirmed that the main specialized metabolites in <i>C. libanotis</i> extract were furanocoumarins, with xanthotoxin, bergapten, and isopimpinellin identified as the three predominant constituents. Treatment with the <i>C. libanotis</i> extract did not induce significant alterations in the adherent human keratinocytes, with confluence and epithelial morphology comparable to control cells. Conversely, viable cells declined in the breast carcinoma cell line (MDA-MB-231). Moreover, the <i>C. libanotis</i> extract exhibited a promising antimicrobial activity against two Gram-negative pathogens, <i>Shigella flexneri</i> and <i>Salmonella typhimurium</i>.https://www.mdpi.com/2076-3921/14/7/810coumarinsphytocomplexescytotoxic effectantibacterial activityirritant potential
spellingShingle Mary Fucile
Ioana Gabriela Macasoi
Monica Negrea
Diana Obistioiu
Mariangela Marrelli
Ersilia Alexa
Cristina Dehelean
Giancarlo Statti
Filomena Conforti
Iulia Pinzaru
Pharmaco-Toxicological Effects of <i>Cachrys libanotis</i> Extract: Antioxidant, Antimicrobial, and Cytotoxic Activities in Human Cell Lines and Embryonic Models
Antioxidants
coumarins
phytocomplexes
cytotoxic effect
antibacterial activity
irritant potential
title Pharmaco-Toxicological Effects of <i>Cachrys libanotis</i> Extract: Antioxidant, Antimicrobial, and Cytotoxic Activities in Human Cell Lines and Embryonic Models
title_full Pharmaco-Toxicological Effects of <i>Cachrys libanotis</i> Extract: Antioxidant, Antimicrobial, and Cytotoxic Activities in Human Cell Lines and Embryonic Models
title_fullStr Pharmaco-Toxicological Effects of <i>Cachrys libanotis</i> Extract: Antioxidant, Antimicrobial, and Cytotoxic Activities in Human Cell Lines and Embryonic Models
title_full_unstemmed Pharmaco-Toxicological Effects of <i>Cachrys libanotis</i> Extract: Antioxidant, Antimicrobial, and Cytotoxic Activities in Human Cell Lines and Embryonic Models
title_short Pharmaco-Toxicological Effects of <i>Cachrys libanotis</i> Extract: Antioxidant, Antimicrobial, and Cytotoxic Activities in Human Cell Lines and Embryonic Models
title_sort pharmaco toxicological effects of i cachrys libanotis i extract antioxidant antimicrobial and cytotoxic activities in human cell lines and embryonic models
topic coumarins
phytocomplexes
cytotoxic effect
antibacterial activity
irritant potential
url https://www.mdpi.com/2076-3921/14/7/810
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