Qualitative and Quantitative Analysis of Volatile Components of Zhengtian Pills Using Gas Chromatography Mass Spectrometry and Ultra-High Performance Liquid Chromatography
Zhengtian pills (ZTPs) are traditional Chinese medicine (TCM) which have been commonly used to treat headaches. Volatile components of ZTPs extracted by ethyl acetate with an ultrasonic method were analyzed by gas chromatography mass spectrometry (GC-MS). Twenty-two components were identified, accou...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2016/1206391 |
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| author | Cui-ting Liu Min Zhang Ping Yan Hai-chan Liu Xing-yun Liu Ruo-ting Zhan |
| author_facet | Cui-ting Liu Min Zhang Ping Yan Hai-chan Liu Xing-yun Liu Ruo-ting Zhan |
| author_sort | Cui-ting Liu |
| collection | DOAJ |
| description | Zhengtian pills (ZTPs) are traditional Chinese medicine (TCM) which have been commonly used to treat headaches. Volatile components of ZTPs extracted by ethyl acetate with an ultrasonic method were analyzed by gas chromatography mass spectrometry (GC-MS). Twenty-two components were identified, accounting for 78.884% of the total components of volatile oil. The three main volatile components including protocatechuic acid, ferulic acid, and ligustilide were simultaneously determined using ultra-high performance liquid chromatography coupled with diode array detection (UHPLC-DAD). Baseline separation was achieved on an XB-C18 column with linear gradient elution of methanol-0.2% acetic acid aqueous solution. The UHPLC-DAD method provided good linearity (R2≥0.9992), precision (RSD < 3%), accuracy (100.68–102.69%), and robustness. The UHPLC-DAD/GC-MS method was successfully utilized to analyze volatile components, protocatechuic acid, ferulic acid, and ligustilide, in 13 batches of ZTPs, which is suitable for discrimination and quality assessment of ZTPs. |
| format | Article |
| id | doaj-art-e541114564e2443fa90fa0830a74c4c6 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-e541114564e2443fa90fa0830a74c4c62025-02-03T05:48:20ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732016-01-01201610.1155/2016/12063911206391Qualitative and Quantitative Analysis of Volatile Components of Zhengtian Pills Using Gas Chromatography Mass Spectrometry and Ultra-High Performance Liquid ChromatographyCui-ting Liu0Min Zhang1Ping Yan2Hai-chan Liu3Xing-yun Liu4Ruo-ting Zhan5Research Center of Chinese Medicinal Resource Science and Engineering, Key Laboratory of Chinese Medicinal Resources from Lingnan of Ministry of Education, Joint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicines, Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong 510006, ChinaResearch Center of Chinese Medicinal Resource Science and Engineering, Key Laboratory of Chinese Medicinal Resources from Lingnan of Ministry of Education, Joint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicines, Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong 510006, ChinaResearch Center of Chinese Medicinal Resource Science and Engineering, Key Laboratory of Chinese Medicinal Resources from Lingnan of Ministry of Education, Joint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicines, Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong 510006, ChinaSchool of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, ChinaResearch Center of Chinese Medicinal Resource Science and Engineering, Key Laboratory of Chinese Medicinal Resources from Lingnan of Ministry of Education, Joint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicines, Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong 510006, ChinaResearch Center of Chinese Medicinal Resource Science and Engineering, Key Laboratory of Chinese Medicinal Resources from Lingnan of Ministry of Education, Joint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicines, Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong 510006, ChinaZhengtian pills (ZTPs) are traditional Chinese medicine (TCM) which have been commonly used to treat headaches. Volatile components of ZTPs extracted by ethyl acetate with an ultrasonic method were analyzed by gas chromatography mass spectrometry (GC-MS). Twenty-two components were identified, accounting for 78.884% of the total components of volatile oil. The three main volatile components including protocatechuic acid, ferulic acid, and ligustilide were simultaneously determined using ultra-high performance liquid chromatography coupled with diode array detection (UHPLC-DAD). Baseline separation was achieved on an XB-C18 column with linear gradient elution of methanol-0.2% acetic acid aqueous solution. The UHPLC-DAD method provided good linearity (R2≥0.9992), precision (RSD < 3%), accuracy (100.68–102.69%), and robustness. The UHPLC-DAD/GC-MS method was successfully utilized to analyze volatile components, protocatechuic acid, ferulic acid, and ligustilide, in 13 batches of ZTPs, which is suitable for discrimination and quality assessment of ZTPs.http://dx.doi.org/10.1155/2016/1206391 |
| spellingShingle | Cui-ting Liu Min Zhang Ping Yan Hai-chan Liu Xing-yun Liu Ruo-ting Zhan Qualitative and Quantitative Analysis of Volatile Components of Zhengtian Pills Using Gas Chromatography Mass Spectrometry and Ultra-High Performance Liquid Chromatography Journal of Analytical Methods in Chemistry |
| title | Qualitative and Quantitative Analysis of Volatile Components of Zhengtian Pills Using Gas Chromatography Mass Spectrometry and Ultra-High Performance Liquid Chromatography |
| title_full | Qualitative and Quantitative Analysis of Volatile Components of Zhengtian Pills Using Gas Chromatography Mass Spectrometry and Ultra-High Performance Liquid Chromatography |
| title_fullStr | Qualitative and Quantitative Analysis of Volatile Components of Zhengtian Pills Using Gas Chromatography Mass Spectrometry and Ultra-High Performance Liquid Chromatography |
| title_full_unstemmed | Qualitative and Quantitative Analysis of Volatile Components of Zhengtian Pills Using Gas Chromatography Mass Spectrometry and Ultra-High Performance Liquid Chromatography |
| title_short | Qualitative and Quantitative Analysis of Volatile Components of Zhengtian Pills Using Gas Chromatography Mass Spectrometry and Ultra-High Performance Liquid Chromatography |
| title_sort | qualitative and quantitative analysis of volatile components of zhengtian pills using gas chromatography mass spectrometry and ultra high performance liquid chromatography |
| url | http://dx.doi.org/10.1155/2016/1206391 |
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