Nucleic acid-binding KH domain proteins influence a spectrum of biological pathways including as part of membrane-localized complexes

K-Homology domain (KH domain) proteins bind single-stranded nucleic acids, influence protein–protein interactions of proteins that harbor them, and are found in all kingdoms of life. In concert with other functional protein domains KH domains contribute to a variety of critical biological activities...

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Main Authors: Md Kamrul Hasan, L. Jeannine Brady
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Journal of Structural Biology: X
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2590152424000114
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author Md Kamrul Hasan
L. Jeannine Brady
author_facet Md Kamrul Hasan
L. Jeannine Brady
author_sort Md Kamrul Hasan
collection DOAJ
description K-Homology domain (KH domain) proteins bind single-stranded nucleic acids, influence protein–protein interactions of proteins that harbor them, and are found in all kingdoms of life. In concert with other functional protein domains KH domains contribute to a variety of critical biological activities, often within higher order machineries including membrane-localized protein complexes. Eukaryotic KH domain proteins are linked to developmental processes, morphogenesis, and growth regulation, and their aberrant expression is often associated with cancer. Prokaryotic KH domain proteins are involved in integral cellular activities including cell division and protein translocation. Eukaryotic and prokaryotic KH domains share structural features, but are differentiated based on their structural organizations. In this review, we explore the structure/function relationships of known examples of KH domain proteins, and highlight cases in which they function within or at membrane surfaces. We also summarize examples of KH domain proteins that influence bacterial virulence and pathogenesis. We conclude the article by discussing prospective research avenues that could be pursued to better investigate this largely understudied protein category.
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spelling doaj-art-e527f396c16f49b3a66ea29ebcd8b4802025-08-20T02:50:27ZengElsevierJournal of Structural Biology: X2590-15242024-12-011010010610.1016/j.yjsbx.2024.100106Nucleic acid-binding KH domain proteins influence a spectrum of biological pathways including as part of membrane-localized complexesMd Kamrul Hasan0L. Jeannine Brady1Department of Oral Biology, University of Florida, Gainesville, FL 32610, USA; Division of Biology, Kansas State University, Manhattan, KS 66506, USADepartment of Oral Biology, University of Florida, Gainesville, FL 32610, USA; Corresponding author at: Department of Oral Biology, University of Florida, P.O. Box 100424, Gainesville, FL 32610, USA.K-Homology domain (KH domain) proteins bind single-stranded nucleic acids, influence protein–protein interactions of proteins that harbor them, and are found in all kingdoms of life. In concert with other functional protein domains KH domains contribute to a variety of critical biological activities, often within higher order machineries including membrane-localized protein complexes. Eukaryotic KH domain proteins are linked to developmental processes, morphogenesis, and growth regulation, and their aberrant expression is often associated with cancer. Prokaryotic KH domain proteins are involved in integral cellular activities including cell division and protein translocation. Eukaryotic and prokaryotic KH domains share structural features, but are differentiated based on their structural organizations. In this review, we explore the structure/function relationships of known examples of KH domain proteins, and highlight cases in which they function within or at membrane surfaces. We also summarize examples of KH domain proteins that influence bacterial virulence and pathogenesis. We conclude the article by discussing prospective research avenues that could be pursued to better investigate this largely understudied protein category.http://www.sciencedirect.com/science/article/pii/S2590152424000114KH domainRNA stabilityBacterial protein translocationMembrane protein complexesProtein-nucleic acid interactions
spellingShingle Md Kamrul Hasan
L. Jeannine Brady
Nucleic acid-binding KH domain proteins influence a spectrum of biological pathways including as part of membrane-localized complexes
Journal of Structural Biology: X
KH domain
RNA stability
Bacterial protein translocation
Membrane protein complexes
Protein-nucleic acid interactions
title Nucleic acid-binding KH domain proteins influence a spectrum of biological pathways including as part of membrane-localized complexes
title_full Nucleic acid-binding KH domain proteins influence a spectrum of biological pathways including as part of membrane-localized complexes
title_fullStr Nucleic acid-binding KH domain proteins influence a spectrum of biological pathways including as part of membrane-localized complexes
title_full_unstemmed Nucleic acid-binding KH domain proteins influence a spectrum of biological pathways including as part of membrane-localized complexes
title_short Nucleic acid-binding KH domain proteins influence a spectrum of biological pathways including as part of membrane-localized complexes
title_sort nucleic acid binding kh domain proteins influence a spectrum of biological pathways including as part of membrane localized complexes
topic KH domain
RNA stability
Bacterial protein translocation
Membrane protein complexes
Protein-nucleic acid interactions
url http://www.sciencedirect.com/science/article/pii/S2590152424000114
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