Investigating the Interaction of Nanodiamonds with Human Serum Albumin and Induced Cytotoxicity
Nanodiamonds (NDs) have been recognized as emerging carbon-based delivery vehicles due to their biocompatibility. NDs were reported to be nontoxic and suited for biomedical applications in the complete cell culture medium. However, in this study, the cytotoxicity of NDs in serum-free medium was stud...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2019/4503137 |
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| _version_ | 1850166905263882240 |
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| author | Miaomiao Chen Xiaoshuang Zuo Qinqin Xu Rong Wang Suhua Fan Hai Wu |
| author_facet | Miaomiao Chen Xiaoshuang Zuo Qinqin Xu Rong Wang Suhua Fan Hai Wu |
| author_sort | Miaomiao Chen |
| collection | DOAJ |
| description | Nanodiamonds (NDs) have been recognized as emerging carbon-based delivery vehicles due to their biocompatibility. NDs were reported to be nontoxic and suited for biomedical applications in the complete cell culture medium. However, in this study, the cytotoxicity of NDs in serum-free medium was studied and indicated that serum proteins in cell culture medium played significant effect on the toxicity of NDs. Therefore, the interaction mechanism between NDs and a serum protein (human serum albumin, HSA) was first investigated by fluorescence quenching technique and circular dichroism (CD) spectrometry. The results suggest that HSA strongly bonds on the NDs surface to form “protein corona,” which not only prevents the aggregation of NDs and improves its stability but also inhibits the cytotoxicity of NDs. In serum-free medium, NDs exhibited obvious toxicity toward the human lung epithelial cell line (BEAS-2B) and showed concentration-dependent cytotoxicity. In the presence of bovine serum albumin (BSA), which shares structural homology and similar properties with HSA, toxicity of NDs was apparently inhibited. Therefore, the interaction between serum protein and NDs should be considered in the understanding of the biological effects of NDs exposure in biomedical applications. |
| format | Article |
| id | doaj-art-e4ff7a91d0ca42cf83a5c957a8b6f31d |
| institution | OA Journals |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-e4ff7a91d0ca42cf83a5c957a8b6f31d2025-08-20T02:21:19ZengWileyJournal of Spectroscopy2314-49202314-49392019-01-01201910.1155/2019/45031374503137Investigating the Interaction of Nanodiamonds with Human Serum Albumin and Induced CytotoxicityMiaomiao Chen0Xiaoshuang Zuo1Qinqin Xu2Rong Wang3Suhua Fan4Hai Wu5School of Chemistry and Materials Engineering, Fuyang Normal University, Fuyang, Anhui 236037, ChinaSchool of Chemistry and Materials Engineering, Fuyang Normal University, Fuyang, Anhui 236037, ChinaSchool of Chemistry and Materials Engineering, Fuyang Normal University, Fuyang, Anhui 236037, ChinaAnhui Province Key Laboratory of Environmental Hormone and Reproduction, Anhui Provincial Key Laboratory for Degradation and Monitoring of Pollution of the Environment, Fuyang Normal University, Fuyang, Anhui 236037, ChinaSchool of Chemistry and Materials Engineering, Fuyang Normal University, Fuyang, Anhui 236037, ChinaSchool of Chemistry and Materials Engineering, Fuyang Normal University, Fuyang, Anhui 236037, ChinaNanodiamonds (NDs) have been recognized as emerging carbon-based delivery vehicles due to their biocompatibility. NDs were reported to be nontoxic and suited for biomedical applications in the complete cell culture medium. However, in this study, the cytotoxicity of NDs in serum-free medium was studied and indicated that serum proteins in cell culture medium played significant effect on the toxicity of NDs. Therefore, the interaction mechanism between NDs and a serum protein (human serum albumin, HSA) was first investigated by fluorescence quenching technique and circular dichroism (CD) spectrometry. The results suggest that HSA strongly bonds on the NDs surface to form “protein corona,” which not only prevents the aggregation of NDs and improves its stability but also inhibits the cytotoxicity of NDs. In serum-free medium, NDs exhibited obvious toxicity toward the human lung epithelial cell line (BEAS-2B) and showed concentration-dependent cytotoxicity. In the presence of bovine serum albumin (BSA), which shares structural homology and similar properties with HSA, toxicity of NDs was apparently inhibited. Therefore, the interaction between serum protein and NDs should be considered in the understanding of the biological effects of NDs exposure in biomedical applications.http://dx.doi.org/10.1155/2019/4503137 |
| spellingShingle | Miaomiao Chen Xiaoshuang Zuo Qinqin Xu Rong Wang Suhua Fan Hai Wu Investigating the Interaction of Nanodiamonds with Human Serum Albumin and Induced Cytotoxicity Journal of Spectroscopy |
| title | Investigating the Interaction of Nanodiamonds with Human Serum Albumin and Induced Cytotoxicity |
| title_full | Investigating the Interaction of Nanodiamonds with Human Serum Albumin and Induced Cytotoxicity |
| title_fullStr | Investigating the Interaction of Nanodiamonds with Human Serum Albumin and Induced Cytotoxicity |
| title_full_unstemmed | Investigating the Interaction of Nanodiamonds with Human Serum Albumin and Induced Cytotoxicity |
| title_short | Investigating the Interaction of Nanodiamonds with Human Serum Albumin and Induced Cytotoxicity |
| title_sort | investigating the interaction of nanodiamonds with human serum albumin and induced cytotoxicity |
| url | http://dx.doi.org/10.1155/2019/4503137 |
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