Response of Soil Bacteria to Short-Term Nitrogen Addition in Nutrient-Poor Areas
Increasing nitrogen (N) addition induces soil nutrient imbalances and is recognized as a major regulator of soil microbial communities. However, how soil bacterial abundance, diversity, and community composition respond to exogenous N addition in nutrient-poor and generally N-limited regions remains...
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2025-01-01
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| author | Hongbin Yin Mingyi Xu Qingyang Huang Lihong Xie Fan Yang Chao Zhang Gang Sha Hongjie Cao |
| author_facet | Hongbin Yin Mingyi Xu Qingyang Huang Lihong Xie Fan Yang Chao Zhang Gang Sha Hongjie Cao |
| author_sort | Hongbin Yin |
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| description | Increasing nitrogen (N) addition induces soil nutrient imbalances and is recognized as a major regulator of soil microbial communities. However, how soil bacterial abundance, diversity, and community composition respond to exogenous N addition in nutrient-poor and generally N-limited regions remains understudied. In this study, we investigated the effects of short-term exogenous N additions on soil bacterial communities using quantitative polymerase chain reaction (PCR) and Illumina Miseq sequencing in an in situ N addition field experiment. The results showed that a low nitrogen addition increased the observed species (Sobs) of the bacterial community, and with the increased nitrogen addition, the Sobs of bacteria gradually decreased, especially the unique OTUs. The relative abundance of <i>Proteobacteria</i>, <i>Actinobacteria</i>, and <i>Gemmatimonadetes</i> increased with increasing nitrogen addition, whereas the relative abundance of <i>Chloroflexi</i> and <i>Firmicutes</i> decreased. Soil properties play an important role in bacterial community structure at phylum or genus levels. Short-term nitrogen addition increased the proportion of nodes from <i>Actinobacteria</i> and <i>Proteobacteria</i> in the co-occurrence network and enhanced the stability of the microbial network. <i>Actinobacteria</i> may play an important role in constructing the network. Our study aims to explore the effects of nitrogen addition on the diversity, composition, and structure of soil bacterial communities in nutrient-poor areas caused by ecological disturbances. |
| format | Article |
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| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-e4f2d124e5454850a04cead41f03ea122025-01-24T13:42:27ZengMDPI AGMicroorganisms2076-26072025-01-011315610.3390/microorganisms13010056Response of Soil Bacteria to Short-Term Nitrogen Addition in Nutrient-Poor AreasHongbin Yin0Mingyi Xu1Qingyang Huang2Lihong Xie3Fan Yang4Chao Zhang5Gang Sha6Hongjie Cao7Institute of Natural Resources and Ecology, Heilongjiang Academy of Science, Harbin 150040, ChinaNational and Local Joint Laboratory of Wetland and Ecological Conservation, Harbin 150040, ChinaInstitute of Natural Resources and Ecology, Heilongjiang Academy of Science, Harbin 150040, ChinaInstitute of Natural Resources and Ecology, Heilongjiang Academy of Science, Harbin 150040, ChinaInstitute of Natural Resources and Ecology, Heilongjiang Academy of Science, Harbin 150040, ChinaInstitute of Natural Resources and Ecology, Heilongjiang Academy of Science, Harbin 150040, ChinaInstitute of Natural Resources and Ecology, Heilongjiang Academy of Science, Harbin 150040, ChinaInstitute of Natural Resources and Ecology, Heilongjiang Academy of Science, Harbin 150040, ChinaIncreasing nitrogen (N) addition induces soil nutrient imbalances and is recognized as a major regulator of soil microbial communities. However, how soil bacterial abundance, diversity, and community composition respond to exogenous N addition in nutrient-poor and generally N-limited regions remains understudied. In this study, we investigated the effects of short-term exogenous N additions on soil bacterial communities using quantitative polymerase chain reaction (PCR) and Illumina Miseq sequencing in an in situ N addition field experiment. The results showed that a low nitrogen addition increased the observed species (Sobs) of the bacterial community, and with the increased nitrogen addition, the Sobs of bacteria gradually decreased, especially the unique OTUs. The relative abundance of <i>Proteobacteria</i>, <i>Actinobacteria</i>, and <i>Gemmatimonadetes</i> increased with increasing nitrogen addition, whereas the relative abundance of <i>Chloroflexi</i> and <i>Firmicutes</i> decreased. Soil properties play an important role in bacterial community structure at phylum or genus levels. Short-term nitrogen addition increased the proportion of nodes from <i>Actinobacteria</i> and <i>Proteobacteria</i> in the co-occurrence network and enhanced the stability of the microbial network. <i>Actinobacteria</i> may play an important role in constructing the network. Our study aims to explore the effects of nitrogen addition on the diversity, composition, and structure of soil bacterial communities in nutrient-poor areas caused by ecological disturbances.https://www.mdpi.com/2076-2607/13/1/56nitrogen additionmicrobial community structurediversitystabilitylava plateau |
| spellingShingle | Hongbin Yin Mingyi Xu Qingyang Huang Lihong Xie Fan Yang Chao Zhang Gang Sha Hongjie Cao Response of Soil Bacteria to Short-Term Nitrogen Addition in Nutrient-Poor Areas Microorganisms nitrogen addition microbial community structure diversity stability lava plateau |
| title | Response of Soil Bacteria to Short-Term Nitrogen Addition in Nutrient-Poor Areas |
| title_full | Response of Soil Bacteria to Short-Term Nitrogen Addition in Nutrient-Poor Areas |
| title_fullStr | Response of Soil Bacteria to Short-Term Nitrogen Addition in Nutrient-Poor Areas |
| title_full_unstemmed | Response of Soil Bacteria to Short-Term Nitrogen Addition in Nutrient-Poor Areas |
| title_short | Response of Soil Bacteria to Short-Term Nitrogen Addition in Nutrient-Poor Areas |
| title_sort | response of soil bacteria to short term nitrogen addition in nutrient poor areas |
| topic | nitrogen addition microbial community structure diversity stability lava plateau |
| url | https://www.mdpi.com/2076-2607/13/1/56 |
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