Identification of quantitative trait loci of pod dehiscence in a collection of soybean grown in the southeast of Kazakhstan
Soybean [Glycine max (L.) Merr.] is one of the important crops that are constantly increasing their cultivation area in Kazakhstan. It is particularly significant in the southeastern regions of the country, which are currently predominant areas for cultivating this crop. One negative trait reducing...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2024-09-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/4233 |
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| author | B. N. Doszhanova A. K. Zatybekov S. V. Didorenko T. Suzuki Y. Yamashita Y. Turuspekov |
| author_facet | B. N. Doszhanova A. K. Zatybekov S. V. Didorenko T. Suzuki Y. Yamashita Y. Turuspekov |
| author_sort | B. N. Doszhanova |
| collection | DOAJ |
| description | Soybean [Glycine max (L.) Merr.] is one of the important crops that are constantly increasing their cultivation area in Kazakhstan. It is particularly significant in the southeastern regions of the country, which are currently predominant areas for cultivating this crop. One negative trait reducing yield in these dry areas is pod dehiscence (PD). Therefore, it is essential to understand the genetic control of PD to breed new cultivars with high yield potential. In this study, we evaluated 273 soybean accessions from different regions of the world for PD resistance in the conditions of southeastern regions of Kazakhstan in 2019 and 2021. The field data for PD suggested that 12 accessions were susceptible to PD in both studied years, and 32 accessions, in one of the two studied years. The genotyping of the collection using a DNA marker for the Pdh1 gene, a major gene for PD, revealed that 244 accessions had the homozygous R (resistant) allele, 14 had the homozygous S (susceptible) allele, and 15 accessions showed heterozygosity. To identify additional quantitative trait loci (QTLs), we applied an association mapping study using a 6K SNP Illumina iSelect array. The results suggested that in addition to major QTL on chromosome 16, linked to the physical location of Pdh1, two minor QTLs were identified on chromosomes 10 and 13. Both minor QTLs for PD were associated with calmodulin-binding protein, which presumably plays an important role in regulating PD in dry areas. Thus, the current study provided additional insight into PD regulation in soybean. The identified QTLs for PD can be efficiently employed in breeding for high-yield soybean cultivars. |
| format | Article |
| id | doaj-art-e4e5b9231208421ebc1199d413751313 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-e4e5b9231208421ebc1199d4137513132025-02-01T09:58:13ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592024-09-0128551552210.18699/vjgb-24-581486Identification of quantitative trait loci of pod dehiscence in a collection of soybean grown in the southeast of KazakhstanB. N. Doszhanova0A. K. Zatybekov1S. V. Didorenko2T. Suzuki3Y. Yamashita4Y. Turuspekov5Institute of Plant Biology and Biotechnology; Al-Farabi Kazakh National UniversityInstitute of Plant Biology and BiotechnologyKazakh Research Institute of Agriculture and Plant GrowingHokkaido Research OrganizationHokkaido Research OrganizationInstitute of Plant Biology and Biotechnology; Al-Farabi Kazakh National UniversitySoybean [Glycine max (L.) Merr.] is one of the important crops that are constantly increasing their cultivation area in Kazakhstan. It is particularly significant in the southeastern regions of the country, which are currently predominant areas for cultivating this crop. One negative trait reducing yield in these dry areas is pod dehiscence (PD). Therefore, it is essential to understand the genetic control of PD to breed new cultivars with high yield potential. In this study, we evaluated 273 soybean accessions from different regions of the world for PD resistance in the conditions of southeastern regions of Kazakhstan in 2019 and 2021. The field data for PD suggested that 12 accessions were susceptible to PD in both studied years, and 32 accessions, in one of the two studied years. The genotyping of the collection using a DNA marker for the Pdh1 gene, a major gene for PD, revealed that 244 accessions had the homozygous R (resistant) allele, 14 had the homozygous S (susceptible) allele, and 15 accessions showed heterozygosity. To identify additional quantitative trait loci (QTLs), we applied an association mapping study using a 6K SNP Illumina iSelect array. The results suggested that in addition to major QTL on chromosome 16, linked to the physical location of Pdh1, two minor QTLs were identified on chromosomes 10 and 13. Both minor QTLs for PD were associated with calmodulin-binding protein, which presumably plays an important role in regulating PD in dry areas. Thus, the current study provided additional insight into PD regulation in soybean. The identified QTLs for PD can be efficiently employed in breeding for high-yield soybean cultivars.https://vavilov.elpub.ru/jour/article/view/4233soybeanpod dehiscenceseed yieldgenome-wide association studyquantitative trait locus |
| spellingShingle | B. N. Doszhanova A. K. Zatybekov S. V. Didorenko T. Suzuki Y. Yamashita Y. Turuspekov Identification of quantitative trait loci of pod dehiscence in a collection of soybean grown in the southeast of Kazakhstan Вавиловский журнал генетики и селекции soybean pod dehiscence seed yield genome-wide association study quantitative trait locus |
| title | Identification of quantitative trait loci of pod dehiscence in a collection of soybean grown in the southeast of Kazakhstan |
| title_full | Identification of quantitative trait loci of pod dehiscence in a collection of soybean grown in the southeast of Kazakhstan |
| title_fullStr | Identification of quantitative trait loci of pod dehiscence in a collection of soybean grown in the southeast of Kazakhstan |
| title_full_unstemmed | Identification of quantitative trait loci of pod dehiscence in a collection of soybean grown in the southeast of Kazakhstan |
| title_short | Identification of quantitative trait loci of pod dehiscence in a collection of soybean grown in the southeast of Kazakhstan |
| title_sort | identification of quantitative trait loci of pod dehiscence in a collection of soybean grown in the southeast of kazakhstan |
| topic | soybean pod dehiscence seed yield genome-wide association study quantitative trait locus |
| url | https://vavilov.elpub.ru/jour/article/view/4233 |
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