The value of fecal calprotectin measured by fluorescent immunochromatography assay in evaluating clinical and endoscopic activity in ulcerative colitis

Abstract Aim Fecal calprotectin (FC) levels correlate with clinical or endoscopic activity in ulcerative colitis (UC), however, these values vary widely between detection methods, and optimal cut-off values remain debated. To assess the correlation between FC levels measured by fluorescent immunochr...

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Main Authors: Mingyang Xu, Junrong Li, Wei Qian, Fangmei Ling, Yidong Chen, Shuang Li, Yiyu Cheng, Qi Yu, Liangru Zhu
Format: Article
Language:English
Published: BMC 2025-08-01
Series:BMC Gastroenterology
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Online Access:https://doi.org/10.1186/s12876-025-04200-7
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author Mingyang Xu
Junrong Li
Wei Qian
Fangmei Ling
Yidong Chen
Shuang Li
Yiyu Cheng
Qi Yu
Liangru Zhu
author_facet Mingyang Xu
Junrong Li
Wei Qian
Fangmei Ling
Yidong Chen
Shuang Li
Yiyu Cheng
Qi Yu
Liangru Zhu
author_sort Mingyang Xu
collection DOAJ
description Abstract Aim Fecal calprotectin (FC) levels correlate with clinical or endoscopic activity in ulcerative colitis (UC), however, these values vary widely between detection methods, and optimal cut-off values remain debated. To assess the correlation between FC levels measured by fluorescent immunochromatography assay (FICA) and disease activity and to identify optimal cut-off values for predicting clinical and endoscopic activity in UC. Method The study included patients hospitalized at Union Hospital, Tongji Medical College, Huazhong University of Science and Technology between January 2021 and June 2022. All the patients had a confirmed diagnosis of UC. Clinical activity was determined using the partial Mayo score (pMS), and endoscopic activity was determined using the ulcerative colitis endoscopy index of severity (UCEIS) and Mayo endoscopic score (MES). Results In 110 patients with UC, FC levels were significantly correlated with pMS (r = 0.609, P < 0.001), UCEIS (r = 0.751, P < 0.001) and MES (r = 0.635, P < 0.001). Moreover, the optimal FC cut-off values to predict clinical activity (pMS 3–12) and endoscopic activity (UCEIS 1–8 or MES 1–3) were 57.38 µg/g and 53.30 µg/g, respectively. Conclusion FC measured by FICA was a good predictor of clinical and endoscopic activity in patients with UC. When using FICA to detect FC, the optimal cut-off value to identify clinical activity in patients with UC was 57.38 µg/g, and a lower value should be chosen to optimize the identification of endoscopic activity in these patients, which was determined as 53.30 µg/g.
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spelling doaj-art-e4dbbc283f794b46ad66fcd1d762a8c02025-08-20T03:05:21ZengBMCBMC Gastroenterology1471-230X2025-08-012511810.1186/s12876-025-04200-7The value of fecal calprotectin measured by fluorescent immunochromatography assay in evaluating clinical and endoscopic activity in ulcerative colitisMingyang Xu0Junrong Li1Wei Qian2Fangmei Ling3Yidong Chen4Shuang Li5Yiyu Cheng6Qi Yu7Liangru Zhu8Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyAbstract Aim Fecal calprotectin (FC) levels correlate with clinical or endoscopic activity in ulcerative colitis (UC), however, these values vary widely between detection methods, and optimal cut-off values remain debated. To assess the correlation between FC levels measured by fluorescent immunochromatography assay (FICA) and disease activity and to identify optimal cut-off values for predicting clinical and endoscopic activity in UC. Method The study included patients hospitalized at Union Hospital, Tongji Medical College, Huazhong University of Science and Technology between January 2021 and June 2022. All the patients had a confirmed diagnosis of UC. Clinical activity was determined using the partial Mayo score (pMS), and endoscopic activity was determined using the ulcerative colitis endoscopy index of severity (UCEIS) and Mayo endoscopic score (MES). Results In 110 patients with UC, FC levels were significantly correlated with pMS (r = 0.609, P < 0.001), UCEIS (r = 0.751, P < 0.001) and MES (r = 0.635, P < 0.001). Moreover, the optimal FC cut-off values to predict clinical activity (pMS 3–12) and endoscopic activity (UCEIS 1–8 or MES 1–3) were 57.38 µg/g and 53.30 µg/g, respectively. Conclusion FC measured by FICA was a good predictor of clinical and endoscopic activity in patients with UC. When using FICA to detect FC, the optimal cut-off value to identify clinical activity in patients with UC was 57.38 µg/g, and a lower value should be chosen to optimize the identification of endoscopic activity in these patients, which was determined as 53.30 µg/g.https://doi.org/10.1186/s12876-025-04200-7Fecal calprotectinUlcerative colitisClinical activityEndoscopic activityCut-off value
spellingShingle Mingyang Xu
Junrong Li
Wei Qian
Fangmei Ling
Yidong Chen
Shuang Li
Yiyu Cheng
Qi Yu
Liangru Zhu
The value of fecal calprotectin measured by fluorescent immunochromatography assay in evaluating clinical and endoscopic activity in ulcerative colitis
BMC Gastroenterology
Fecal calprotectin
Ulcerative colitis
Clinical activity
Endoscopic activity
Cut-off value
title The value of fecal calprotectin measured by fluorescent immunochromatography assay in evaluating clinical and endoscopic activity in ulcerative colitis
title_full The value of fecal calprotectin measured by fluorescent immunochromatography assay in evaluating clinical and endoscopic activity in ulcerative colitis
title_fullStr The value of fecal calprotectin measured by fluorescent immunochromatography assay in evaluating clinical and endoscopic activity in ulcerative colitis
title_full_unstemmed The value of fecal calprotectin measured by fluorescent immunochromatography assay in evaluating clinical and endoscopic activity in ulcerative colitis
title_short The value of fecal calprotectin measured by fluorescent immunochromatography assay in evaluating clinical and endoscopic activity in ulcerative colitis
title_sort value of fecal calprotectin measured by fluorescent immunochromatography assay in evaluating clinical and endoscopic activity in ulcerative colitis
topic Fecal calprotectin
Ulcerative colitis
Clinical activity
Endoscopic activity
Cut-off value
url https://doi.org/10.1186/s12876-025-04200-7
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