Growth and Metabolite Enhancement of Acidophile Euglena sp. Isolated from Indonesia under Different Photoperiod Cycles
Abstract Euglena sp. is a unicellular, flagellated microalga considered one of the most promising microalgal feedstock species for biofuels. Reducing the level of liquid waste pollutants can be done biologically by using microalgal organisms. Its metabolites, including lipids, proteins,...
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Faculty of Fisheries and Marine Universitas Airlangga
2023-12-01
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| Series: | Jurnal Ilmiah Perikanan dan Kelautan |
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| Online Access: | https://e-journal.unair.ac.id/JIPK/article/view/46193 |
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| author | Tia Erfianti Budi Setiadi Daryono Arief Budiman Eko Agus Suyono |
| author_facet | Tia Erfianti Budi Setiadi Daryono Arief Budiman Eko Agus Suyono |
| author_sort | Tia Erfianti |
| collection | DOAJ |
| description | Abstract
Euglena sp. is a unicellular, flagellated microalga considered one of the most promising microalgal feedstock species for biofuels. Reducing the level of liquid waste pollutants can be done biologically by using microalgal organisms. Its metabolites, including lipids, proteins, carbohydrates, and pigments, are appropriate for producing biorefinery products such as biodiesel and jet fuels. They can be isolated from extreme environments, such as highly acidic and ammonia-rich environments, that are not conducive to their proliferation. This study sought to determine the effect of the photoperiod or (light: dark) cycle (24 L:0 D, 12 L:12 D, 14 L:10 D, and 16 L:8 D) on the growth, biomass, metabolite content consisting of lipids, carbohydrates, and proteins, and the rate of CO2 uptake by Euglena sp. As stated previously, the study was conducted by cultivating Euglena sp. on a laboratory scale with four photoperiod regimens. The results indicated that optimal growth, biomass content, and metabolite content were obtained with a 24 D:0 L lighting cycle. The control treatment (24 L: 0 D) had the highest biomass productivity (0.032 g.L-1.day-1 ± 0.004), lipid content (0.387 g.L-1 ± 0.031), protein content (0.542 mg.Ml-1 ± 0.007), carbohydrate content (0.409 x104 g.L-1), chlorophyll a (6.237 g.L-1 ± 0.184), chlorophyll b (2.838 g.L-1 ± 0.253), and total carotenoid (1.566 g.L-1 ± 0.105). Full light illumination (24 L:0 D) was significantly producing carotenoid content, including phaeophytin a, phaeophytin b, violaxanthin, 9'-cis-neoxanthin, dino xanthin, and fucoxanthin.
Highlight Research
• The growth rate was higher when Euglena was cultivated under continuous illumination.
• The biomass productivity of Euglena increased significantly under continuous illumination.
• The metabolite content of Euglena (lipids, proteins, and pigments) was higher in continuous illumination. |
| format | Article |
| id | doaj-art-e4d9dbaaa4374ba8bfdf9f87e78ef43b |
| institution | OA Journals |
| issn | 2085-5842 2528-0759 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Faculty of Fisheries and Marine Universitas Airlangga |
| record_format | Article |
| series | Jurnal Ilmiah Perikanan dan Kelautan |
| spelling | doaj-art-e4d9dbaaa4374ba8bfdf9f87e78ef43b2025-08-20T02:21:18ZengFaculty of Fisheries and Marine Universitas AirlanggaJurnal Ilmiah Perikanan dan Kelautan2085-58422528-07592023-12-01161153010.20473/jipk.v16i1.4619344279Growth and Metabolite Enhancement of Acidophile Euglena sp. Isolated from Indonesia under Different Photoperiod CyclesTia Erfianti0https://orcid.org/0000-0002-5462-1131Budi Setiadi Daryono1https://orcid.org/0000-0002-0703-2123Arief Budiman2https://orcid.org/0000-0003-4846-8270Eko Agus Suyono3https://orcid.org/0000-0002-9208-4541Faculty of Biology, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. IndonesiaFaculty of Biology, Universitas Gadjah MadaFaculty of Biology, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. IndonesiaDepartment of Chemical Engineering, Faculty of Engineering, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. Indonesia; Center of Excellent of Microalgae, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. IndonesiaFaculty of Biology, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. Indonesia; Center of Excellent of Microalgae, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. IndonesiaAbstract Euglena sp. is a unicellular, flagellated microalga considered one of the most promising microalgal feedstock species for biofuels. Reducing the level of liquid waste pollutants can be done biologically by using microalgal organisms. Its metabolites, including lipids, proteins, carbohydrates, and pigments, are appropriate for producing biorefinery products such as biodiesel and jet fuels. They can be isolated from extreme environments, such as highly acidic and ammonia-rich environments, that are not conducive to their proliferation. This study sought to determine the effect of the photoperiod or (light: dark) cycle (24 L:0 D, 12 L:12 D, 14 L:10 D, and 16 L:8 D) on the growth, biomass, metabolite content consisting of lipids, carbohydrates, and proteins, and the rate of CO2 uptake by Euglena sp. As stated previously, the study was conducted by cultivating Euglena sp. on a laboratory scale with four photoperiod regimens. The results indicated that optimal growth, biomass content, and metabolite content were obtained with a 24 D:0 L lighting cycle. The control treatment (24 L: 0 D) had the highest biomass productivity (0.032 g.L-1.day-1 ± 0.004), lipid content (0.387 g.L-1 ± 0.031), protein content (0.542 mg.Ml-1 ± 0.007), carbohydrate content (0.409 x104 g.L-1), chlorophyll a (6.237 g.L-1 ± 0.184), chlorophyll b (2.838 g.L-1 ± 0.253), and total carotenoid (1.566 g.L-1 ± 0.105). Full light illumination (24 L:0 D) was significantly producing carotenoid content, including phaeophytin a, phaeophytin b, violaxanthin, 9'-cis-neoxanthin, dino xanthin, and fucoxanthin. Highlight Research • The growth rate was higher when Euglena was cultivated under continuous illumination. • The biomass productivity of Euglena increased significantly under continuous illumination. • The metabolite content of Euglena (lipids, proteins, and pigments) was higher in continuous illumination.https://e-journal.unair.ac.id/JIPK/article/view/46193carotenoidseuglena sp.growth ratemetabolitesphotoperiod |
| spellingShingle | Tia Erfianti Budi Setiadi Daryono Arief Budiman Eko Agus Suyono Growth and Metabolite Enhancement of Acidophile Euglena sp. Isolated from Indonesia under Different Photoperiod Cycles Jurnal Ilmiah Perikanan dan Kelautan carotenoids euglena sp. growth rate metabolites photoperiod |
| title | Growth and Metabolite Enhancement of Acidophile Euglena sp. Isolated from Indonesia under Different Photoperiod Cycles |
| title_full | Growth and Metabolite Enhancement of Acidophile Euglena sp. Isolated from Indonesia under Different Photoperiod Cycles |
| title_fullStr | Growth and Metabolite Enhancement of Acidophile Euglena sp. Isolated from Indonesia under Different Photoperiod Cycles |
| title_full_unstemmed | Growth and Metabolite Enhancement of Acidophile Euglena sp. Isolated from Indonesia under Different Photoperiod Cycles |
| title_short | Growth and Metabolite Enhancement of Acidophile Euglena sp. Isolated from Indonesia under Different Photoperiod Cycles |
| title_sort | growth and metabolite enhancement of acidophile euglena sp isolated from indonesia under different photoperiod cycles |
| topic | carotenoids euglena sp. growth rate metabolites photoperiod |
| url | https://e-journal.unair.ac.id/JIPK/article/view/46193 |
| work_keys_str_mv | AT tiaerfianti growthandmetaboliteenhancementofacidophileeuglenaspisolatedfromindonesiaunderdifferentphotoperiodcycles AT budisetiadidaryono growthandmetaboliteenhancementofacidophileeuglenaspisolatedfromindonesiaunderdifferentphotoperiodcycles AT ariefbudiman growthandmetaboliteenhancementofacidophileeuglenaspisolatedfromindonesiaunderdifferentphotoperiodcycles AT ekoagussuyono growthandmetaboliteenhancementofacidophileeuglenaspisolatedfromindonesiaunderdifferentphotoperiodcycles |