Morphologic confounders and CD19 negativity in a case of hairy cell leukemia.
Objectives:We report a case of hairy cell leukemia (HCL) initially misdiagnosed as plasma cell dyscrasia due to various clinical, morphological and immunophenotypic confounders. Methods and results:In a patient diagnosed of marrow plasmacytosis and serum monoclonal protein elsewhere and referred to...
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| Language: | English |
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PAGEPress Publications
2017-05-01
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| Series: | Mediterranean Journal of Hematology and Infectious Diseases |
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| Online Access: | http://www.mjhid.org/index.php/mjhid/article/view/2882 |
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| author | Pulkit Rastogi Sreejesh Sreedharanunni Uday Yanamandra Man Updesh Singh Sachdeva Neelam Varma |
| author_facet | Pulkit Rastogi Sreejesh Sreedharanunni Uday Yanamandra Man Updesh Singh Sachdeva Neelam Varma |
| author_sort | Pulkit Rastogi |
| collection | DOAJ |
| description | Objectives:We report a case of hairy cell leukemia (HCL) initially misdiagnosed as plasma cell dyscrasia due to various clinical, morphological and immunophenotypic confounders.
Methods and results:In a patient diagnosed of marrow plasmacytosis and serum monoclonal protein elsewhere and referred to our hospital, morphological evaluation of bone marrow aspirate smears and trephine biopsy, immunophenotyping, and molecular testing (BRAFV600E mutation) were done. Clinically, the patient was asymptomatic, bone marrow revealed plasmacytosis, mastocytosis and lymphocytosis with a few “hairy” cells. Immunophenotyping revealed features of HCL with aberrant CD10 expression and a subclone of CD19neg cells. A diagnosis of HCL with reactive plasmacytosis and mast cell hyperplasia was made and confirmed by immunophenotyping and molecular studies.
Conclusion:Hematopathologists must be aware of various confounding factors and should judiciously use flow cytometric and molecular studies for attaining a proper diagnosis of HCL. We also report a very rare immunophenotypic aberrancy (CD 19 negativity) in HCL |
| format | Article |
| id | doaj-art-e4d7cb2a1f3849c89ee74d0a76e5e9f6 |
| institution | OA Journals |
| issn | 2035-3006 |
| language | English |
| publishDate | 2017-05-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Mediterranean Journal of Hematology and Infectious Diseases |
| spelling | doaj-art-e4d7cb2a1f3849c89ee74d0a76e5e9f62025-08-20T01:47:47ZengPAGEPress PublicationsMediterranean Journal of Hematology and Infectious Diseases2035-30062017-05-0191e2017033e201703310.4084/mjhid.2017.0331687Morphologic confounders and CD19 negativity in a case of hairy cell leukemia.Pulkit Rastogi0Sreejesh Sreedharanunni1Uday Yanamandra2Man Updesh Singh Sachdeva3Neelam Varma4Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, India- 160012Postgraduate Institute of Medical Education and Research, Chandigarh, India-160012Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, India- 160012Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, India- 160012Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, India- 160012Objectives:We report a case of hairy cell leukemia (HCL) initially misdiagnosed as plasma cell dyscrasia due to various clinical, morphological and immunophenotypic confounders. Methods and results:In a patient diagnosed of marrow plasmacytosis and serum monoclonal protein elsewhere and referred to our hospital, morphological evaluation of bone marrow aspirate smears and trephine biopsy, immunophenotyping, and molecular testing (BRAFV600E mutation) were done. Clinically, the patient was asymptomatic, bone marrow revealed plasmacytosis, mastocytosis and lymphocytosis with a few “hairy” cells. Immunophenotyping revealed features of HCL with aberrant CD10 expression and a subclone of CD19neg cells. A diagnosis of HCL with reactive plasmacytosis and mast cell hyperplasia was made and confirmed by immunophenotyping and molecular studies. Conclusion:Hematopathologists must be aware of various confounding factors and should judiciously use flow cytometric and molecular studies for attaining a proper diagnosis of HCL. We also report a very rare immunophenotypic aberrancy (CD 19 negativity) in HCLhttp://www.mjhid.org/index.php/mjhid/article/view/2882Hairy cell leukemiaAberrancyImmunophenotypeCD19 negativity. |
| spellingShingle | Pulkit Rastogi Sreejesh Sreedharanunni Uday Yanamandra Man Updesh Singh Sachdeva Neelam Varma Morphologic confounders and CD19 negativity in a case of hairy cell leukemia. Mediterranean Journal of Hematology and Infectious Diseases Hairy cell leukemia Aberrancy Immunophenotype CD19 negativity. |
| title | Morphologic confounders and CD19 negativity in a case of hairy cell leukemia. |
| title_full | Morphologic confounders and CD19 negativity in a case of hairy cell leukemia. |
| title_fullStr | Morphologic confounders and CD19 negativity in a case of hairy cell leukemia. |
| title_full_unstemmed | Morphologic confounders and CD19 negativity in a case of hairy cell leukemia. |
| title_short | Morphologic confounders and CD19 negativity in a case of hairy cell leukemia. |
| title_sort | morphologic confounders and cd19 negativity in a case of hairy cell leukemia |
| topic | Hairy cell leukemia Aberrancy Immunophenotype CD19 negativity. |
| url | http://www.mjhid.org/index.php/mjhid/article/view/2882 |
| work_keys_str_mv | AT pulkitrastogi morphologicconfoundersandcd19negativityinacaseofhairycellleukemia AT sreejeshsreedharanunni morphologicconfoundersandcd19negativityinacaseofhairycellleukemia AT udayyanamandra morphologicconfoundersandcd19negativityinacaseofhairycellleukemia AT manupdeshsinghsachdeva morphologicconfoundersandcd19negativityinacaseofhairycellleukemia AT neelamvarma morphologicconfoundersandcd19negativityinacaseofhairycellleukemia |