Whole organism and tissue-specific analysis of pexophagy in Drosophila
Peroxisomes are essential organelles involved in critical metabolic processes in animals such as fatty acid oxidation, ether phospholipid production and reactive oxygen species detoxification. We have generated transgenic Drosophila melanogaster models expressing fluorescent reporters for the select...
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The Royal Society
2025-02-01
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| Series: | Open Biology |
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| Online Access: | https://royalsocietypublishing.org/doi/10.1098/rsob.240291 |
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| author | Francesco G. Barone Marco Marcello Sylvie Urbé Natalia Sanchez-Soriano Michael J. Clague |
| author_facet | Francesco G. Barone Marco Marcello Sylvie Urbé Natalia Sanchez-Soriano Michael J. Clague |
| author_sort | Francesco G. Barone |
| collection | DOAJ |
| description | Peroxisomes are essential organelles involved in critical metabolic processes in animals such as fatty acid oxidation, ether phospholipid production and reactive oxygen species detoxification. We have generated transgenic Drosophila melanogaster models expressing fluorescent reporters for the selective autophagy of peroxisomes, a process known as pexophagy. We show that these reporters are colocalized with a peroxisomal marker and that they can reflect pexophagy induction by iron chelation and inhibition by depletion of the core autophagy protein Atg5. Using light sheet microscopy, we have been able to obtain a global overview of pexophagy levels across the entire organism at different stages of development. Tissue-specific control of pexophagy is exemplified by areas of peroxisome abundance but minimal pexophagy, observed in clusters of oenocytes surrounded by epithelial cells where pexophagy is much more evident. Enhancement of pexophagy was achieved by feeding flies with the iron chelator deferiprone, in line with past results using mammalian cells. Specific drivers were used to visualize pexophagy in neurons, and to demonstrate that specific depletion in the larval central nervous system of Hsc70-5, the Drosophila homologue of the chaperone HSPA9/mortalin, led to a substantial elevation in pexophagy. |
| format | Article |
| id | doaj-art-e4bdc70475214dd782f27c331dd29071 |
| institution | Kabale University |
| issn | 2046-2441 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | The Royal Society |
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| series | Open Biology |
| spelling | doaj-art-e4bdc70475214dd782f27c331dd290712025-02-05T00:05:20ZengThe Royal SocietyOpen Biology2046-24412025-02-0115210.1098/rsob.240291Whole organism and tissue-specific analysis of pexophagy in DrosophilaFrancesco G. Barone0Marco Marcello1Sylvie Urbé2Natalia Sanchez-Soriano3Michael J. Clague4Department of Biochemistry, Cell and Systems Biology, ISMIB, University of Liverpool, Liverpool L69 3BX, UKCentre for Cell Imaging, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool L69 7ZB, UKDepartment of Biochemistry, Cell and Systems Biology, ISMIB, University of Liverpool, Liverpool L69 3BX, UKDepartment of Biochemistry, Cell and Systems Biology, ISMIB, University of Liverpool, Liverpool L69 3BX, UKDepartment of Biochemistry, Cell and Systems Biology, ISMIB, University of Liverpool, Liverpool L69 3BX, UKPeroxisomes are essential organelles involved in critical metabolic processes in animals such as fatty acid oxidation, ether phospholipid production and reactive oxygen species detoxification. We have generated transgenic Drosophila melanogaster models expressing fluorescent reporters for the selective autophagy of peroxisomes, a process known as pexophagy. We show that these reporters are colocalized with a peroxisomal marker and that they can reflect pexophagy induction by iron chelation and inhibition by depletion of the core autophagy protein Atg5. Using light sheet microscopy, we have been able to obtain a global overview of pexophagy levels across the entire organism at different stages of development. Tissue-specific control of pexophagy is exemplified by areas of peroxisome abundance but minimal pexophagy, observed in clusters of oenocytes surrounded by epithelial cells where pexophagy is much more evident. Enhancement of pexophagy was achieved by feeding flies with the iron chelator deferiprone, in line with past results using mammalian cells. Specific drivers were used to visualize pexophagy in neurons, and to demonstrate that specific depletion in the larval central nervous system of Hsc70-5, the Drosophila homologue of the chaperone HSPA9/mortalin, led to a substantial elevation in pexophagy.https://royalsocietypublishing.org/doi/10.1098/rsob.240291pexophagyperoxisomesmortalinHsc70-5neuronsDrosophila |
| spellingShingle | Francesco G. Barone Marco Marcello Sylvie Urbé Natalia Sanchez-Soriano Michael J. Clague Whole organism and tissue-specific analysis of pexophagy in Drosophila Open Biology pexophagy peroxisomes mortalin Hsc70-5 neurons Drosophila |
| title | Whole organism and tissue-specific analysis of pexophagy in Drosophila |
| title_full | Whole organism and tissue-specific analysis of pexophagy in Drosophila |
| title_fullStr | Whole organism and tissue-specific analysis of pexophagy in Drosophila |
| title_full_unstemmed | Whole organism and tissue-specific analysis of pexophagy in Drosophila |
| title_short | Whole organism and tissue-specific analysis of pexophagy in Drosophila |
| title_sort | whole organism and tissue specific analysis of pexophagy in drosophila |
| topic | pexophagy peroxisomes mortalin Hsc70-5 neurons Drosophila |
| url | https://royalsocietypublishing.org/doi/10.1098/rsob.240291 |
| work_keys_str_mv | AT francescogbarone wholeorganismandtissuespecificanalysisofpexophagyindrosophila AT marcomarcello wholeorganismandtissuespecificanalysisofpexophagyindrosophila AT sylvieurbe wholeorganismandtissuespecificanalysisofpexophagyindrosophila AT nataliasanchezsoriano wholeorganismandtissuespecificanalysisofpexophagyindrosophila AT michaeljclague wholeorganismandtissuespecificanalysisofpexophagyindrosophila |