Genome-wide analysis of MYB transcription factors in four Rheum L. plants provides new insights into the synthesis of Anthraquinones
R. tanguticum (Rheum tanguticum Maxim. ex Regel) is a herbaceous plant belonging to Polygonaceae family and Rheum L. genus. It holds considerable value in culinary and medicinal realms, primarily due to their rich Anthraquinones (AQs) content. Understanding the molecular mechanisms that regulate AQs...
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1558321/full |
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| author | Tao Wang Tao Wang Shuo Zhao Shuo Zhao Bo Wang Bo Wang Jianan Li Jianan Li Zengrong Ye Zengrong Ye Famei Zhang Famei Zhang Huiyuan Ma Huiyuan Ma Guoying Zhou Guoying Zhou |
| author_facet | Tao Wang Tao Wang Shuo Zhao Shuo Zhao Bo Wang Bo Wang Jianan Li Jianan Li Zengrong Ye Zengrong Ye Famei Zhang Famei Zhang Huiyuan Ma Huiyuan Ma Guoying Zhou Guoying Zhou |
| author_sort | Tao Wang |
| collection | DOAJ |
| description | R. tanguticum (Rheum tanguticum Maxim. ex Regel) is a herbaceous plant belonging to Polygonaceae family and Rheum L. genus. It holds considerable value in culinary and medicinal realms, primarily due to their rich Anthraquinones (AQs) content. Understanding the molecular mechanisms that regulate AQs biosynthesis is a prerequisite for increasing their yield. MYB transcription factors (TFs) can regulate the synthesis of a variety of plant secondary metabolites. However, only a few research have explored the role of MYB TFs in Rheum L. species. In this study, 1054 MYB genes from four Rheum L. species were identified. The number of MYB genes in each species was similar, distributed across 11 chromosomes. To investigate the phylogeny of identified MYB TFs, they were classified into four subfamilies. Sequence characteristics, phylogenetic relationships, evolutionary trends, and tissue expression of MYB genes in Rheum L. species were further studied. Subsequently, 12 MYB genes were selected, which shown differential expression in different tissues. Further research on these genes indicated a significant correlation with genes in shikimate pathway and polyketide pathway of AQs biosynthesis. Protein-protein interaction simulations in Arabidopsis thaliana and qRT-PCR experiments further confirmed this situation. This research lays the foundation for studying molecular mechanisms by which MYB TFs regulates AQs biosynthesis in four Rheum L. species. |
| format | Article |
| id | doaj-art-e4b5806a6dfe41aab0b2ed96faa72e14 |
| institution | OA Journals |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-e4b5806a6dfe41aab0b2ed96faa72e142025-08-20T02:31:45ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-05-011610.3389/fpls.2025.15583211558321Genome-wide analysis of MYB transcription factors in four Rheum L. plants provides new insights into the synthesis of AnthraquinonesTao Wang0Tao Wang1Shuo Zhao2Shuo Zhao3Bo Wang4Bo Wang5Jianan Li6Jianan Li7Zengrong Ye8Zengrong Ye9Famei Zhang10Famei Zhang11Huiyuan Ma12Huiyuan Ma13Guoying Zhou14Guoying Zhou15Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, ChinaCollege of Agriculture and Animal Husbandry, Qinghai University, Xining, ChinaKey Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, ChinaCollege of Life Sciences, Qinghai Normal University, Xining, ChinaKey Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaR. tanguticum (Rheum tanguticum Maxim. ex Regel) is a herbaceous plant belonging to Polygonaceae family and Rheum L. genus. It holds considerable value in culinary and medicinal realms, primarily due to their rich Anthraquinones (AQs) content. Understanding the molecular mechanisms that regulate AQs biosynthesis is a prerequisite for increasing their yield. MYB transcription factors (TFs) can regulate the synthesis of a variety of plant secondary metabolites. However, only a few research have explored the role of MYB TFs in Rheum L. species. In this study, 1054 MYB genes from four Rheum L. species were identified. The number of MYB genes in each species was similar, distributed across 11 chromosomes. To investigate the phylogeny of identified MYB TFs, they were classified into four subfamilies. Sequence characteristics, phylogenetic relationships, evolutionary trends, and tissue expression of MYB genes in Rheum L. species were further studied. Subsequently, 12 MYB genes were selected, which shown differential expression in different tissues. Further research on these genes indicated a significant correlation with genes in shikimate pathway and polyketide pathway of AQs biosynthesis. Protein-protein interaction simulations in Arabidopsis thaliana and qRT-PCR experiments further confirmed this situation. This research lays the foundation for studying molecular mechanisms by which MYB TFs regulates AQs biosynthesis in four Rheum L. species.https://www.frontiersin.org/articles/10.3389/fpls.2025.1558321/fullMYBsanthraquinonesgenome-wide analysisRNA-SeqqPCRRheum L. plants |
| spellingShingle | Tao Wang Tao Wang Shuo Zhao Shuo Zhao Bo Wang Bo Wang Jianan Li Jianan Li Zengrong Ye Zengrong Ye Famei Zhang Famei Zhang Huiyuan Ma Huiyuan Ma Guoying Zhou Guoying Zhou Genome-wide analysis of MYB transcription factors in four Rheum L. plants provides new insights into the synthesis of Anthraquinones Frontiers in Plant Science MYBs anthraquinones genome-wide analysis RNA-Seq qPCR Rheum L. plants |
| title | Genome-wide analysis of MYB transcription factors in four Rheum L. plants provides new insights into the synthesis of Anthraquinones |
| title_full | Genome-wide analysis of MYB transcription factors in four Rheum L. plants provides new insights into the synthesis of Anthraquinones |
| title_fullStr | Genome-wide analysis of MYB transcription factors in four Rheum L. plants provides new insights into the synthesis of Anthraquinones |
| title_full_unstemmed | Genome-wide analysis of MYB transcription factors in four Rheum L. plants provides new insights into the synthesis of Anthraquinones |
| title_short | Genome-wide analysis of MYB transcription factors in four Rheum L. plants provides new insights into the synthesis of Anthraquinones |
| title_sort | genome wide analysis of myb transcription factors in four rheum l plants provides new insights into the synthesis of anthraquinones |
| topic | MYBs anthraquinones genome-wide analysis RNA-Seq qPCR Rheum L. plants |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1558321/full |
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