Knocking Down the DRD2 by shRNA Expressing Plasmids in the Nucleus Accumbens Prevented the Disrupting Effect of Apomorphine on Prepulse Inhibition in Rat
Prepulse Inhibition (PPI), the objective measure of sensorimotor gating disturbance has being widely used in animal models of schizophrenia. Dopaminergic direct and indirect agonists impair PPI. However, the profile of dopaminergic receptors involved in PPI impairment by dopamine agonists is not cle...
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University of Tehran
2015-09-01
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| Series: | Journal of Sciences, Islamic Republic of Iran |
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| Online Access: | https://jsciences.ut.ac.ir/article_55308_eb4b1150fb252aa03c8ffd6d54239943.pdf |
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| author | M.R. Noori-daloii A. Shahbazi S. Alizadeh Zendehrood A. Shayan Nia M. Mojarrad M. Kheirollahi A. Rashidi-Nezhad A. Haghparast F. Zare Mehrjerdi S. Niknazar M. Mostowfi H. Hashemi Sotoobadi M. Khaksari A. R. Noori-Daloii A. Mohammadi |
| author_facet | M.R. Noori-daloii A. Shahbazi S. Alizadeh Zendehrood A. Shayan Nia M. Mojarrad M. Kheirollahi A. Rashidi-Nezhad A. Haghparast F. Zare Mehrjerdi S. Niknazar M. Mostowfi H. Hashemi Sotoobadi M. Khaksari A. R. Noori-Daloii A. Mohammadi |
| author_sort | M.R. Noori-daloii |
| collection | DOAJ |
| description | Prepulse Inhibition (PPI), the objective measure of sensorimotor gating disturbance has being widely used in animal models of schizophrenia. Dopaminergic direct and indirect agonists impair PPI. However, the profile of dopaminergic receptors involved in PPI impairment by dopamine agonists is not clear. By injecting shRNA expressing plasmids against dopamine D2 receptor genes (DRD2) in the nucleus accumbens, here, we studied the effect of apomorphine on PPI in D2 down-regulated rats. Seventy two adult Wistar rats assigned randomly in nine groups, each received coding (250 and 500 ng/µl) or noncoding shRNA expressing plasmids against DRD2 in the nucleus accumbens, with or without apomorphine (0.5 mg/kg, S.C., 72 hours after treatment with plasmids). Auditory startle response and PPI were measured after apomorphine injection. Real time RT-PCR was used to measure DRD2 expression. Results showed that apomorphine significantly decreased PPI in noncoding plasmid treated rats; While, PPI did not impaired in rats pretreated with 250 and 500 ng/µl shRNA expressing plasmids. Accordingly, the expression of DRD2 mRNA in the nucleus accumbens showed 72-78% decrease in expressing plasmid treated rats. Additionally, treatment with expressing plasmids had no effect on basal PPI and/or auditory startle response. Taken together, our results demonstrated that DRD2 silencing in the nucleus accumbens can prevent PPI impairment by apomorphine. These observations suggest application of molecular techniques such as the use of shRNA against DRD2s in studies of schizophrenia pathophysiology and development of new treatments in schizophrenia. |
| format | Article |
| id | doaj-art-e45fd27fef1b4fcf8d455250d3383f2c |
| institution | Kabale University |
| issn | 1016-1104 2345-6914 |
| language | English |
| publishDate | 2015-09-01 |
| publisher | University of Tehran |
| record_format | Article |
| series | Journal of Sciences, Islamic Republic of Iran |
| spelling | doaj-art-e45fd27fef1b4fcf8d455250d3383f2c2025-08-20T03:54:07ZengUniversity of TehranJournal of Sciences, Islamic Republic of Iran1016-11042345-69142015-09-0126320521255308Knocking Down the DRD2 by shRNA Expressing Plasmids in the Nucleus Accumbens Prevented the Disrupting Effect of Apomorphine on Prepulse Inhibition in RatM.R. Noori-daloii0A. Shahbazi1S. Alizadeh Zendehrood2A. Shayan Nia3M. Mojarrad4M. Kheirollahi5A. Rashidi-Nezhad6A. Haghparast7F. Zare Mehrjerdi8S. Niknazar9M. Mostowfi10H. Hashemi Sotoobadi11M. Khaksari12A. R. Noori-Daloii13A. Mohammadi14Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of IranPhysiology Research Center, Iran University of Medical Sciences, Tehran, Islamic Republic of Iran.Physiology Research Center, Iran University of Medical Sciences, Tehran, Islamic Republic of Iran.Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of IranDepartment of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of IranDepartment of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of IranDepartment of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran.Neuroscience Research Center, ShahidBeheshti University of Medical Sciences, Tehran, Islamic Republic of Iran.Physiology Research Center, Iran University of Medical Sciences, Tehran, Islamic Republic of Iran.Physiology Research Center, Iran University of Medical Sciences, Tehran, Islamic Republic of Iran.Physiology Research Center, Iran University of Medical Sciences, Tehran, Islamic Republic of Iran.Physiology Research Center, Iran University of Medical Sciences, Tehran, Islamic Republic of Iran.Physiology Department, Shahrood University of Medical Sciences, Shahrood, Islamic Republic of Iran.Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran.Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Islamic Republic of Iran.Prepulse Inhibition (PPI), the objective measure of sensorimotor gating disturbance has being widely used in animal models of schizophrenia. Dopaminergic direct and indirect agonists impair PPI. However, the profile of dopaminergic receptors involved in PPI impairment by dopamine agonists is not clear. By injecting shRNA expressing plasmids against dopamine D2 receptor genes (DRD2) in the nucleus accumbens, here, we studied the effect of apomorphine on PPI in D2 down-regulated rats. Seventy two adult Wistar rats assigned randomly in nine groups, each received coding (250 and 500 ng/µl) or noncoding shRNA expressing plasmids against DRD2 in the nucleus accumbens, with or without apomorphine (0.5 mg/kg, S.C., 72 hours after treatment with plasmids). Auditory startle response and PPI were measured after apomorphine injection. Real time RT-PCR was used to measure DRD2 expression. Results showed that apomorphine significantly decreased PPI in noncoding plasmid treated rats; While, PPI did not impaired in rats pretreated with 250 and 500 ng/µl shRNA expressing plasmids. Accordingly, the expression of DRD2 mRNA in the nucleus accumbens showed 72-78% decrease in expressing plasmid treated rats. Additionally, treatment with expressing plasmids had no effect on basal PPI and/or auditory startle response. Taken together, our results demonstrated that DRD2 silencing in the nucleus accumbens can prevent PPI impairment by apomorphine. These observations suggest application of molecular techniques such as the use of shRNA against DRD2s in studies of schizophrenia pathophysiology and development of new treatments in schizophrenia.https://jsciences.ut.ac.ir/article_55308_eb4b1150fb252aa03c8ffd6d54239943.pdfshrnadrd2nucleus accumbensschizophreniaprepulse inhibition |
| spellingShingle | M.R. Noori-daloii A. Shahbazi S. Alizadeh Zendehrood A. Shayan Nia M. Mojarrad M. Kheirollahi A. Rashidi-Nezhad A. Haghparast F. Zare Mehrjerdi S. Niknazar M. Mostowfi H. Hashemi Sotoobadi M. Khaksari A. R. Noori-Daloii A. Mohammadi Knocking Down the DRD2 by shRNA Expressing Plasmids in the Nucleus Accumbens Prevented the Disrupting Effect of Apomorphine on Prepulse Inhibition in Rat Journal of Sciences, Islamic Republic of Iran shrna drd2 nucleus accumbens schizophrenia prepulse inhibition |
| title | Knocking Down the DRD2 by shRNA Expressing Plasmids in the Nucleus Accumbens Prevented the Disrupting Effect of Apomorphine on Prepulse Inhibition in Rat |
| title_full | Knocking Down the DRD2 by shRNA Expressing Plasmids in the Nucleus Accumbens Prevented the Disrupting Effect of Apomorphine on Prepulse Inhibition in Rat |
| title_fullStr | Knocking Down the DRD2 by shRNA Expressing Plasmids in the Nucleus Accumbens Prevented the Disrupting Effect of Apomorphine on Prepulse Inhibition in Rat |
| title_full_unstemmed | Knocking Down the DRD2 by shRNA Expressing Plasmids in the Nucleus Accumbens Prevented the Disrupting Effect of Apomorphine on Prepulse Inhibition in Rat |
| title_short | Knocking Down the DRD2 by shRNA Expressing Plasmids in the Nucleus Accumbens Prevented the Disrupting Effect of Apomorphine on Prepulse Inhibition in Rat |
| title_sort | knocking down the drd2 by shrna expressing plasmids in the nucleus accumbens prevented the disrupting effect of apomorphine on prepulse inhibition in rat |
| topic | shrna drd2 nucleus accumbens schizophrenia prepulse inhibition |
| url | https://jsciences.ut.ac.ir/article_55308_eb4b1150fb252aa03c8ffd6d54239943.pdf |
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