<i>Bacillus velezensis</i> TCS001 Enhances the Resistance of Hickory to <i>Phytophthora cinnamomi</i> and Reshapes the Rhizosphere Microbial Community
<i>Phytophthora cinnamomi</i> causes significant root rot in hickory, leading to substantial yield losses. While <i>Bacillus</i> spp. are recognized as beneficial rhizosphere microorganisms, their application against hickory root rot and their impact on rhizosphere microbial...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Agriculture |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0472/15/2/193 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832589508769480704 |
|---|---|
| author | Chenshun Xie Yuntian Wu Zhonghao Wu Hao Cao Xiaohui Huang Feng Cui Shuai Meng Jie Chen |
| author_facet | Chenshun Xie Yuntian Wu Zhonghao Wu Hao Cao Xiaohui Huang Feng Cui Shuai Meng Jie Chen |
| author_sort | Chenshun Xie |
| collection | DOAJ |
| description | <i>Phytophthora cinnamomi</i> causes significant root rot in hickory, leading to substantial yield losses. While <i>Bacillus</i> spp. are recognized as beneficial rhizosphere microorganisms, their application against hickory root rot and their impact on rhizosphere microbial communities remain under-investigated. This study demonstrated that <i>Bacillus velezensis</i> TCS001 significantly inhibited <i>P. cinnamomi</i> ST402 growth in vitro, and achieved 71% efficacy in root rot disease management. Scanning electron microscopy (SEM) revealed that TCS001 fermentation filtrate induced mycelial deformities in <i>P. cinnamomi</i>. An analysis of <i>α</i> and <i>β</i> diversity indicated a significant impact of TCS001 on rhizosphere bacterial community richness and diversity, with minimal effects on the fungal community. Moreover, TCS001 altered the hickory rhizosphere microbiome co-occurrence network. The differential abundance analysis suggests that TCS001 promotes the recruitment of beneficial microbes associated with disease resistance, thereby suppressing disease development. These findings underscore the influence of TCS001 on the hickory rhizosphere microbiome in the presence of pathogens, providing valuable data for future research and the development of effective biocontrol strategies for hickory root rot. |
| format | Article |
| id | doaj-art-e44e253e4a4c49fea68486b4db7475a6 |
| institution | Kabale University |
| issn | 2077-0472 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj-art-e44e253e4a4c49fea68486b4db7475a62025-01-24T13:16:04ZengMDPI AGAgriculture2077-04722025-01-0115219310.3390/agriculture15020193<i>Bacillus velezensis</i> TCS001 Enhances the Resistance of Hickory to <i>Phytophthora cinnamomi</i> and Reshapes the Rhizosphere Microbial CommunityChenshun Xie0Yuntian Wu1Zhonghao Wu2Hao Cao3Xiaohui Huang4Feng Cui5Shuai Meng6Jie Chen7National Joint Local Engineering Laboratory for High-Efficient Preparation of Biopesticide, College of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, ChinaNational Joint Local Engineering Laboratory for High-Efficient Preparation of Biopesticide, College of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, ChinaNational Joint Local Engineering Laboratory for High-Efficient Preparation of Biopesticide, College of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, ChinaNational Joint Local Engineering Laboratory for High-Efficient Preparation of Biopesticide, College of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, ChinaNational Joint Local Engineering Laboratory for High-Efficient Preparation of Biopesticide, College of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, ChinaNational Joint Local Engineering Laboratory for High-Efficient Preparation of Biopesticide, College of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, ChinaNational Joint Local Engineering Laboratory for High-Efficient Preparation of Biopesticide, College of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, ChinaNational Joint Local Engineering Laboratory for High-Efficient Preparation of Biopesticide, College of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China<i>Phytophthora cinnamomi</i> causes significant root rot in hickory, leading to substantial yield losses. While <i>Bacillus</i> spp. are recognized as beneficial rhizosphere microorganisms, their application against hickory root rot and their impact on rhizosphere microbial communities remain under-investigated. This study demonstrated that <i>Bacillus velezensis</i> TCS001 significantly inhibited <i>P. cinnamomi</i> ST402 growth in vitro, and achieved 71% efficacy in root rot disease management. Scanning electron microscopy (SEM) revealed that TCS001 fermentation filtrate induced mycelial deformities in <i>P. cinnamomi</i>. An analysis of <i>α</i> and <i>β</i> diversity indicated a significant impact of TCS001 on rhizosphere bacterial community richness and diversity, with minimal effects on the fungal community. Moreover, TCS001 altered the hickory rhizosphere microbiome co-occurrence network. The differential abundance analysis suggests that TCS001 promotes the recruitment of beneficial microbes associated with disease resistance, thereby suppressing disease development. These findings underscore the influence of TCS001 on the hickory rhizosphere microbiome in the presence of pathogens, providing valuable data for future research and the development of effective biocontrol strategies for hickory root rot.https://www.mdpi.com/2077-0472/15/2/193<i>Phytophthora cinnamomi</i><i>Bacillus velezensis</i> TCS001<i>Carya cathayensis</i>root rotrhizosphere microbial |
| spellingShingle | Chenshun Xie Yuntian Wu Zhonghao Wu Hao Cao Xiaohui Huang Feng Cui Shuai Meng Jie Chen <i>Bacillus velezensis</i> TCS001 Enhances the Resistance of Hickory to <i>Phytophthora cinnamomi</i> and Reshapes the Rhizosphere Microbial Community Agriculture <i>Phytophthora cinnamomi</i> <i>Bacillus velezensis</i> TCS001 <i>Carya cathayensis</i> root rot rhizosphere microbial |
| title | <i>Bacillus velezensis</i> TCS001 Enhances the Resistance of Hickory to <i>Phytophthora cinnamomi</i> and Reshapes the Rhizosphere Microbial Community |
| title_full | <i>Bacillus velezensis</i> TCS001 Enhances the Resistance of Hickory to <i>Phytophthora cinnamomi</i> and Reshapes the Rhizosphere Microbial Community |
| title_fullStr | <i>Bacillus velezensis</i> TCS001 Enhances the Resistance of Hickory to <i>Phytophthora cinnamomi</i> and Reshapes the Rhizosphere Microbial Community |
| title_full_unstemmed | <i>Bacillus velezensis</i> TCS001 Enhances the Resistance of Hickory to <i>Phytophthora cinnamomi</i> and Reshapes the Rhizosphere Microbial Community |
| title_short | <i>Bacillus velezensis</i> TCS001 Enhances the Resistance of Hickory to <i>Phytophthora cinnamomi</i> and Reshapes the Rhizosphere Microbial Community |
| title_sort | i bacillus velezensis i tcs001 enhances the resistance of hickory to i phytophthora cinnamomi i and reshapes the rhizosphere microbial community |
| topic | <i>Phytophthora cinnamomi</i> <i>Bacillus velezensis</i> TCS001 <i>Carya cathayensis</i> root rot rhizosphere microbial |
| url | https://www.mdpi.com/2077-0472/15/2/193 |
| work_keys_str_mv | AT chenshunxie ibacillusvelezensisitcs001enhancestheresistanceofhickorytoiphytophthoracinnamomiiandreshapestherhizospheremicrobialcommunity AT yuntianwu ibacillusvelezensisitcs001enhancestheresistanceofhickorytoiphytophthoracinnamomiiandreshapestherhizospheremicrobialcommunity AT zhonghaowu ibacillusvelezensisitcs001enhancestheresistanceofhickorytoiphytophthoracinnamomiiandreshapestherhizospheremicrobialcommunity AT haocao ibacillusvelezensisitcs001enhancestheresistanceofhickorytoiphytophthoracinnamomiiandreshapestherhizospheremicrobialcommunity AT xiaohuihuang ibacillusvelezensisitcs001enhancestheresistanceofhickorytoiphytophthoracinnamomiiandreshapestherhizospheremicrobialcommunity AT fengcui ibacillusvelezensisitcs001enhancestheresistanceofhickorytoiphytophthoracinnamomiiandreshapestherhizospheremicrobialcommunity AT shuaimeng ibacillusvelezensisitcs001enhancestheresistanceofhickorytoiphytophthoracinnamomiiandreshapestherhizospheremicrobialcommunity AT jiechen ibacillusvelezensisitcs001enhancestheresistanceofhickorytoiphytophthoracinnamomiiandreshapestherhizospheremicrobialcommunity |