Effects of Pesticides on Occupationally Exposed Humans
Pesticides are known to contain numerous genotoxic compounds; however, genotoxicity biomonitoring studies of workers occupationally exposed to pesticides have produced variable results. In this study, we employed the Comet assay to examine DNA damage in peripheral blood lymphocytes (PBLs) from 64 gr...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2006-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2006.207 |
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| author | Stylianos M. Piperakis Konstantina Kontogianni Michael M. Piperakis Ricardo Marcos Smaragdi Tsilimigaki |
| author_facet | Stylianos M. Piperakis Konstantina Kontogianni Michael M. Piperakis Ricardo Marcos Smaragdi Tsilimigaki |
| author_sort | Stylianos M. Piperakis |
| collection | DOAJ |
| description | Pesticides are known to contain numerous genotoxic compounds; however, genotoxicity biomonitoring studies of workers occupationally exposed to pesticides have produced variable results. In this study, we employed the Comet assay to examine DNA damage in peripheral blood lymphocytes (PBLs) from 64 greenhouse workers from Almería in south-eastern Spain in comparison to PBLs from 50 men from the same area but not engaged in any agricultural work. The results indicated that there were no differences in the basal levels of DNA damage in the two study groups. In addition, exposure of PBL from the workers and controls to hydrogen peroxide or γ-irradiation led to similar levels of DNA damage; the subsequent repair of the induced DNA damage was also similar for both study populations. Smoking had no impact on any of the responses. The results of this study indicate that the greenhouse workers had no detectable increase in DNA damage or alteration in the cellular response to DNA damage compared to our control population. |
| format | Article |
| id | doaj-art-e431983a16184bbb835c433a2cb21470 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2006-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-e431983a16184bbb835c433a2cb214702025-02-03T01:32:38ZengWileyThe Scientific World Journal1537-744X2006-01-0161211122010.1100/tsw.2006.207Effects of Pesticides on Occupationally Exposed HumansStylianos M. Piperakis0Konstantina Kontogianni1Michael M. Piperakis2Ricardo Marcos3Smaragdi Tsilimigaki4Biology Unit, Department of Pre-School, Education University of Thessaly, Volos 38221, GreeceBiology Unit, Department of Pre-School, Education University of Thessaly, Volos 38221, GreeceDepartment of Chemistry, University of Sheffield, United KingdomGrup de Mutagenesi, Departament de Genetica I de Microbiologia, Universitat Autonoma de Barcelona, 08193 Bellaterra, SpainDNA Repair Laboratory, Institute of Biology, National Center for Scientific Research “Demokritos”, 153 10 Aghia Paraskevi, Athens, GreecePesticides are known to contain numerous genotoxic compounds; however, genotoxicity biomonitoring studies of workers occupationally exposed to pesticides have produced variable results. In this study, we employed the Comet assay to examine DNA damage in peripheral blood lymphocytes (PBLs) from 64 greenhouse workers from Almería in south-eastern Spain in comparison to PBLs from 50 men from the same area but not engaged in any agricultural work. The results indicated that there were no differences in the basal levels of DNA damage in the two study groups. In addition, exposure of PBL from the workers and controls to hydrogen peroxide or γ-irradiation led to similar levels of DNA damage; the subsequent repair of the induced DNA damage was also similar for both study populations. Smoking had no impact on any of the responses. The results of this study indicate that the greenhouse workers had no detectable increase in DNA damage or alteration in the cellular response to DNA damage compared to our control population.http://dx.doi.org/10.1100/tsw.2006.207 |
| spellingShingle | Stylianos M. Piperakis Konstantina Kontogianni Michael M. Piperakis Ricardo Marcos Smaragdi Tsilimigaki Effects of Pesticides on Occupationally Exposed Humans The Scientific World Journal |
| title | Effects of Pesticides on Occupationally Exposed Humans |
| title_full | Effects of Pesticides on Occupationally Exposed Humans |
| title_fullStr | Effects of Pesticides on Occupationally Exposed Humans |
| title_full_unstemmed | Effects of Pesticides on Occupationally Exposed Humans |
| title_short | Effects of Pesticides on Occupationally Exposed Humans |
| title_sort | effects of pesticides on occupationally exposed humans |
| url | http://dx.doi.org/10.1100/tsw.2006.207 |
| work_keys_str_mv | AT stylianosmpiperakis effectsofpesticidesonoccupationallyexposedhumans AT konstantinakontogianni effectsofpesticidesonoccupationallyexposedhumans AT michaelmpiperakis effectsofpesticidesonoccupationallyexposedhumans AT ricardomarcos effectsofpesticidesonoccupationallyexposedhumans AT smaragditsilimigaki effectsofpesticidesonoccupationallyexposedhumans |