Impact of Arbuscular Mycorrhizal Symbiosis on Photosynthetic, Antioxidant Enzyme, and Water Flux Parameters in Salt-Stressed Chickpea (<i>Cicer arietinum</i>) Plants
Globally, escalating soil salinization poses significant abiotic stress, disproportionately impacting crops like chickpea (<i>Cicer arientinum</i> L.). This legume exhibits high sensitivity to salinity, which disrupts various physiological and metabolic processes, ultimately hindering gr...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/15/1/247 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832589438057709568 |
|---|---|
| author | Pooja Pooja Sarita Devi Sridevi Tallapragada Yogesh K. Ahlawat Nisha Sharma Pankaj Kasnia Nita Lakra Rosa Porcel Jose M. Mulet Khalid M. Elhindi |
| author_facet | Pooja Pooja Sarita Devi Sridevi Tallapragada Yogesh K. Ahlawat Nisha Sharma Pankaj Kasnia Nita Lakra Rosa Porcel Jose M. Mulet Khalid M. Elhindi |
| author_sort | Pooja Pooja |
| collection | DOAJ |
| description | Globally, escalating soil salinization poses significant abiotic stress, disproportionately impacting crops like chickpea (<i>Cicer arientinum</i> L.). This legume exhibits high sensitivity to salinity, which disrupts various physiological and metabolic processes, ultimately hindering growth and productivity. AMF (arbuscular mycorrhizal fungus) reduces salt’s detrimental effects on plants’ growth by bolstering the plant’s antioxidant defense system, effectively reducing the damage caused by oxidative stress. In this study, the impact of AMF on salinity stress alleviation in chickpea was investigated in pot-grown experiments. <i>Rhizophagus fasciculatus</i> was used to inoculate the seeds of three different chickpea varieties (HC-3, CSG-8962, and C-235), and the physiological and biochemical changes of the AMF-inoculated and non-inoculated chickpea plants were studied. When exposed to salinity stress, the plants exhibited decreased leaf relative water content (RWC %) (21.13–31.30%), increased leaf relative stress injury, decreased chlorophyll content (45.22–58.24%), photochemical quantum yield, photosynthetic rate, transpiration rate, and stomatal conductance as compared to the control plants, but opposite results were observed in AMF colonized plants. A 9.16% to 14.79% increase in chlorophyll content was reported after AMF colonization. The activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POX) were increased by salt stress. They were further enhanced by AMF inoculation SOD activity by 20.3% to 23.3%, CAT activity by 65.7% to 78.7%, and POX activity by 32.7% to 39.3%. The findings clearly show that AMF <i>Rhizophagus fasciculatus</i>, via enhancing RWC, photosynthetic parameters, and antioxidant enzymes, can mitigate salinity stress in chickpeas. |
| format | Article |
| id | doaj-art-e412a5f41d68433cabf9eb23427549b0 |
| institution | Kabale University |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj-art-e412a5f41d68433cabf9eb23427549b02025-01-24T13:17:19ZengMDPI AGAgronomy2073-43952025-01-0115124710.3390/agronomy15010247Impact of Arbuscular Mycorrhizal Symbiosis on Photosynthetic, Antioxidant Enzyme, and Water Flux Parameters in Salt-Stressed Chickpea (<i>Cicer arietinum</i>) PlantsPooja Pooja0Sarita Devi1Sridevi Tallapragada2Yogesh K. Ahlawat3Nisha Sharma4Pankaj Kasnia5Nita Lakra6Rosa Porcel7Jose M. Mulet8Khalid M. Elhindi9Department of Botany and Plant Physiology, CCS Haryana Agricultural University, Hisar 125001, Haryana, IndiaDepartment of Botany and Plant Physiology, CCS Haryana Agricultural University, Hisar 125001, Haryana, IndiaDepartment of Botany and Plant Physiology, CCS Haryana Agricultural University, Hisar 125001, Haryana, IndiaDepartment of Biotechnology, University Centre for Research and Development, Chandigarh University, Gharuan, Mohali 140413, Punjab, IndiaDepartment of Biotechnology, Graphic Era (Deemed to Be University), Dehradun 248002, Uttarakhand, IndiaDepartment of Botany and Plant Physiology, CCS Haryana Agricultural University, Hisar 125001, Haryana, IndiaDepartment of MBB, College of Biotechnology, CCS Haryana Agricultural University, Hisar 125001, Haryana, IndiaInstituto de Biología Molecular y Celular de Plantas (IBMCP), Universitat Politècnica de València-Consejo Superior de Investigaciones Científicas, Avd. de los Naranjos s/n, 46022 Valencia, SpainInstituto de Biología Molecular y Celular de Plantas (IBMCP), Universitat Politècnica de València-Consejo Superior de Investigaciones Científicas, Avd. de los Naranjos s/n, 46022 Valencia, SpainDepartment of Plant Production, College of Food & Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaGlobally, escalating soil salinization poses significant abiotic stress, disproportionately impacting crops like chickpea (<i>Cicer arientinum</i> L.). This legume exhibits high sensitivity to salinity, which disrupts various physiological and metabolic processes, ultimately hindering growth and productivity. AMF (arbuscular mycorrhizal fungus) reduces salt’s detrimental effects on plants’ growth by bolstering the plant’s antioxidant defense system, effectively reducing the damage caused by oxidative stress. In this study, the impact of AMF on salinity stress alleviation in chickpea was investigated in pot-grown experiments. <i>Rhizophagus fasciculatus</i> was used to inoculate the seeds of three different chickpea varieties (HC-3, CSG-8962, and C-235), and the physiological and biochemical changes of the AMF-inoculated and non-inoculated chickpea plants were studied. When exposed to salinity stress, the plants exhibited decreased leaf relative water content (RWC %) (21.13–31.30%), increased leaf relative stress injury, decreased chlorophyll content (45.22–58.24%), photochemical quantum yield, photosynthetic rate, transpiration rate, and stomatal conductance as compared to the control plants, but opposite results were observed in AMF colonized plants. A 9.16% to 14.79% increase in chlorophyll content was reported after AMF colonization. The activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POX) were increased by salt stress. They were further enhanced by AMF inoculation SOD activity by 20.3% to 23.3%, CAT activity by 65.7% to 78.7%, and POX activity by 32.7% to 39.3%. The findings clearly show that AMF <i>Rhizophagus fasciculatus</i>, via enhancing RWC, photosynthetic parameters, and antioxidant enzymes, can mitigate salinity stress in chickpeas.https://www.mdpi.com/2073-4395/15/1/247chickpeasalt stressarbuscular mycorrhizaantioxidant enzymeschlorophyll |
| spellingShingle | Pooja Pooja Sarita Devi Sridevi Tallapragada Yogesh K. Ahlawat Nisha Sharma Pankaj Kasnia Nita Lakra Rosa Porcel Jose M. Mulet Khalid M. Elhindi Impact of Arbuscular Mycorrhizal Symbiosis on Photosynthetic, Antioxidant Enzyme, and Water Flux Parameters in Salt-Stressed Chickpea (<i>Cicer arietinum</i>) Plants Agronomy chickpea salt stress arbuscular mycorrhiza antioxidant enzymes chlorophyll |
| title | Impact of Arbuscular Mycorrhizal Symbiosis on Photosynthetic, Antioxidant Enzyme, and Water Flux Parameters in Salt-Stressed Chickpea (<i>Cicer arietinum</i>) Plants |
| title_full | Impact of Arbuscular Mycorrhizal Symbiosis on Photosynthetic, Antioxidant Enzyme, and Water Flux Parameters in Salt-Stressed Chickpea (<i>Cicer arietinum</i>) Plants |
| title_fullStr | Impact of Arbuscular Mycorrhizal Symbiosis on Photosynthetic, Antioxidant Enzyme, and Water Flux Parameters in Salt-Stressed Chickpea (<i>Cicer arietinum</i>) Plants |
| title_full_unstemmed | Impact of Arbuscular Mycorrhizal Symbiosis on Photosynthetic, Antioxidant Enzyme, and Water Flux Parameters in Salt-Stressed Chickpea (<i>Cicer arietinum</i>) Plants |
| title_short | Impact of Arbuscular Mycorrhizal Symbiosis on Photosynthetic, Antioxidant Enzyme, and Water Flux Parameters in Salt-Stressed Chickpea (<i>Cicer arietinum</i>) Plants |
| title_sort | impact of arbuscular mycorrhizal symbiosis on photosynthetic antioxidant enzyme and water flux parameters in salt stressed chickpea i cicer arietinum i plants |
| topic | chickpea salt stress arbuscular mycorrhiza antioxidant enzymes chlorophyll |
| url | https://www.mdpi.com/2073-4395/15/1/247 |
| work_keys_str_mv | AT poojapooja impactofarbuscularmycorrhizalsymbiosisonphotosyntheticantioxidantenzymeandwaterfluxparametersinsaltstressedchickpeaicicerarietinumiplants AT saritadevi impactofarbuscularmycorrhizalsymbiosisonphotosyntheticantioxidantenzymeandwaterfluxparametersinsaltstressedchickpeaicicerarietinumiplants AT sridevitallapragada impactofarbuscularmycorrhizalsymbiosisonphotosyntheticantioxidantenzymeandwaterfluxparametersinsaltstressedchickpeaicicerarietinumiplants AT yogeshkahlawat impactofarbuscularmycorrhizalsymbiosisonphotosyntheticantioxidantenzymeandwaterfluxparametersinsaltstressedchickpeaicicerarietinumiplants AT nishasharma impactofarbuscularmycorrhizalsymbiosisonphotosyntheticantioxidantenzymeandwaterfluxparametersinsaltstressedchickpeaicicerarietinumiplants AT pankajkasnia impactofarbuscularmycorrhizalsymbiosisonphotosyntheticantioxidantenzymeandwaterfluxparametersinsaltstressedchickpeaicicerarietinumiplants AT nitalakra impactofarbuscularmycorrhizalsymbiosisonphotosyntheticantioxidantenzymeandwaterfluxparametersinsaltstressedchickpeaicicerarietinumiplants AT rosaporcel impactofarbuscularmycorrhizalsymbiosisonphotosyntheticantioxidantenzymeandwaterfluxparametersinsaltstressedchickpeaicicerarietinumiplants AT josemmulet impactofarbuscularmycorrhizalsymbiosisonphotosyntheticantioxidantenzymeandwaterfluxparametersinsaltstressedchickpeaicicerarietinumiplants AT khalidmelhindi impactofarbuscularmycorrhizalsymbiosisonphotosyntheticantioxidantenzymeandwaterfluxparametersinsaltstressedchickpeaicicerarietinumiplants |