A Microfluidic Design for Quantitative Measurements of Shear Stress-Dependent Adhesion and Motion of <i>Dictyostelium discoideum</i> Cells
Shear stress plays a crucial role in modulating cell adhesion and signaling. We present a microfluidic shear stress generator used to investigate the adhesion dynamics of <i>Dictyostelium discoideum</i>, an amoeba cell model organism with well-characterized adhesion properties. We applie...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-10-01
|
| Series: | Biomimetics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2313-7673/9/11/657 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850145402640138240 |
|---|---|
| author | Sepideh Fakhari Clémence Belleannée Steve J. Charrette Jesse Greener |
| author_facet | Sepideh Fakhari Clémence Belleannée Steve J. Charrette Jesse Greener |
| author_sort | Sepideh Fakhari |
| collection | DOAJ |
| description | Shear stress plays a crucial role in modulating cell adhesion and signaling. We present a microfluidic shear stress generator used to investigate the adhesion dynamics of <i>Dictyostelium discoideum</i>, an amoeba cell model organism with well-characterized adhesion properties. We applied shear stress and tracked cell adhesion, motility, and detachment using time-lapse videomicroscopy. In the precise shear conditions generated on-chip, our results show cell migration patterns are influenced by shear stress, with cells displaying an adaptive response to shear forces as they alter their adhesion and motility behavior. Additionally, we observed that DH1-10 wild-type <i>D. discoideum</i> cells exhibit stronger adhesion and resistance to shear-induced detachment compared to <i>phg2</i> adhesion-defective mutant cells. We also highlight the influence of cell density on detachment kinetics. |
| format | Article |
| id | doaj-art-e409a91ef3ea4bc8861167db85aebe91 |
| institution | OA Journals |
| issn | 2313-7673 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomimetics |
| spelling | doaj-art-e409a91ef3ea4bc8861167db85aebe912025-08-20T02:28:07ZengMDPI AGBiomimetics2313-76732024-10-0191165710.3390/biomimetics9110657A Microfluidic Design for Quantitative Measurements of Shear Stress-Dependent Adhesion and Motion of <i>Dictyostelium discoideum</i> CellsSepideh Fakhari0Clémence Belleannée1Steve J. Charrette2Jesse Greener3Department of Chemistry, Faculty of Science and Engineering, Université Laval, Québec City, QC G1V 0A6, CanadaCentre de Recherche du Centre Hospitalier Universitaire de Québec, Université Laval, Québec City, QC G1V 0A6, CanadaDepartment of Biochemistry, Microbiology and Bioinformatics, Faculty of Science and Engineering, Université Laval, Québec City, QC G1V 0A6, CanadaDepartment of Chemistry, Faculty of Science and Engineering, Université Laval, Québec City, QC G1V 0A6, CanadaShear stress plays a crucial role in modulating cell adhesion and signaling. We present a microfluidic shear stress generator used to investigate the adhesion dynamics of <i>Dictyostelium discoideum</i>, an amoeba cell model organism with well-characterized adhesion properties. We applied shear stress and tracked cell adhesion, motility, and detachment using time-lapse videomicroscopy. In the precise shear conditions generated on-chip, our results show cell migration patterns are influenced by shear stress, with cells displaying an adaptive response to shear forces as they alter their adhesion and motility behavior. Additionally, we observed that DH1-10 wild-type <i>D. discoideum</i> cells exhibit stronger adhesion and resistance to shear-induced detachment compared to <i>phg2</i> adhesion-defective mutant cells. We also highlight the influence of cell density on detachment kinetics.https://www.mdpi.com/2313-7673/9/11/657cell adhesioncell motilityshear stressmicrofluidic devicesamoeba<i>Dictyostelium discoideum</i> |
| spellingShingle | Sepideh Fakhari Clémence Belleannée Steve J. Charrette Jesse Greener A Microfluidic Design for Quantitative Measurements of Shear Stress-Dependent Adhesion and Motion of <i>Dictyostelium discoideum</i> Cells Biomimetics cell adhesion cell motility shear stress microfluidic devices amoeba <i>Dictyostelium discoideum</i> |
| title | A Microfluidic Design for Quantitative Measurements of Shear Stress-Dependent Adhesion and Motion of <i>Dictyostelium discoideum</i> Cells |
| title_full | A Microfluidic Design for Quantitative Measurements of Shear Stress-Dependent Adhesion and Motion of <i>Dictyostelium discoideum</i> Cells |
| title_fullStr | A Microfluidic Design for Quantitative Measurements of Shear Stress-Dependent Adhesion and Motion of <i>Dictyostelium discoideum</i> Cells |
| title_full_unstemmed | A Microfluidic Design for Quantitative Measurements of Shear Stress-Dependent Adhesion and Motion of <i>Dictyostelium discoideum</i> Cells |
| title_short | A Microfluidic Design for Quantitative Measurements of Shear Stress-Dependent Adhesion and Motion of <i>Dictyostelium discoideum</i> Cells |
| title_sort | microfluidic design for quantitative measurements of shear stress dependent adhesion and motion of i dictyostelium discoideum i cells |
| topic | cell adhesion cell motility shear stress microfluidic devices amoeba <i>Dictyostelium discoideum</i> |
| url | https://www.mdpi.com/2313-7673/9/11/657 |
| work_keys_str_mv | AT sepidehfakhari amicrofluidicdesignforquantitativemeasurementsofshearstressdependentadhesionandmotionofidictyosteliumdiscoideumicells AT clemencebelleannee amicrofluidicdesignforquantitativemeasurementsofshearstressdependentadhesionandmotionofidictyosteliumdiscoideumicells AT stevejcharrette amicrofluidicdesignforquantitativemeasurementsofshearstressdependentadhesionandmotionofidictyosteliumdiscoideumicells AT jessegreener amicrofluidicdesignforquantitativemeasurementsofshearstressdependentadhesionandmotionofidictyosteliumdiscoideumicells AT sepidehfakhari microfluidicdesignforquantitativemeasurementsofshearstressdependentadhesionandmotionofidictyosteliumdiscoideumicells AT clemencebelleannee microfluidicdesignforquantitativemeasurementsofshearstressdependentadhesionandmotionofidictyosteliumdiscoideumicells AT stevejcharrette microfluidicdesignforquantitativemeasurementsofshearstressdependentadhesionandmotionofidictyosteliumdiscoideumicells AT jessegreener microfluidicdesignforquantitativemeasurementsofshearstressdependentadhesionandmotionofidictyosteliumdiscoideumicells |