Generation of Chicken IgY against SARS-COV-2 Spike Protein and Epitope Mapping
This new decade has started with a global pandemic of COVID-19 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), precipitating a worldwide health crisis and economic downturn. Scientists and clinicians have been racing against time to find therapies for COVID-19. Repurposing ap...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/9465398 |
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| author | Yan Lu Yajun Wang Zhen Zhang Jingliang Huang Meicun Yao Guobin Huang Yuanyuan Ge Peichun Zhang Huaxin Huang Yong Wang Huiliang Li Wen Wang |
| author_facet | Yan Lu Yajun Wang Zhen Zhang Jingliang Huang Meicun Yao Guobin Huang Yuanyuan Ge Peichun Zhang Huaxin Huang Yong Wang Huiliang Li Wen Wang |
| author_sort | Yan Lu |
| collection | DOAJ |
| description | This new decade has started with a global pandemic of COVID-19 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), precipitating a worldwide health crisis and economic downturn. Scientists and clinicians have been racing against time to find therapies for COVID-19. Repurposing approved drugs, developing vaccines and employing passive immunization are three major therapeutic approaches to fighting COVID-19. Chicken immunoglobulin Y (IgY) has the potential to be used as neutralizing antibody against respiratory infections, and its advantages include high avidity, low risk of adverse immune responses, and easy local delivery by intranasal administration. In this study, we raised antibody against the spike (S) protein of SARS-CoV-2 in chickens and extracted IgY (called IgY-S) from egg yolk. IgY-S exhibited high immunoreactivity against SARS-CoV-2 S, and by epitope mapping, we found five linear epitopes of IgY-S in SARS-CoV-2 S, two of which are cross-reactive with SARS-CoV S. Notably, epitope SIIAYTMSL, one of the identified epitopes, partially overlaps the S1/S2 cleavage region in SARS-CoV-2 S and is located on the surface of S trimer in 3D structure, close to the S1/S2 cleavage site. Thus, antibody binding at this location could physically block the access of proteolytic enzymes to S1/S2 cleavage site and thereby impede S1/S2 proteolytic cleavage, which is crucial to subsequent virus-cell membrane fusion and viral cell entry. Therefore, the feasibility of using IgY-S or epitope SIIAYTMS-specific IgY as neutralizing antibody for preventing or treating SARS-CoV-2 infection is worth exploring. |
| format | Article |
| id | doaj-art-e3e81709c6da44de980e866dd4329b0c |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-e3e81709c6da44de980e866dd4329b0c2025-02-03T01:05:15ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/94653989465398Generation of Chicken IgY against SARS-COV-2 Spike Protein and Epitope MappingYan Lu0Yajun Wang1Zhen Zhang2Jingliang Huang3Meicun Yao4Guobin Huang5Yuanyuan Ge6Peichun Zhang7Huaxin Huang8Yong Wang9Huiliang Li10Wen Wang11Huamin Medicine Co Ltd., Zhuhai, ChinaWolfson Institute for Biomedical Research, University College London, London, UKJoint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, ChinaSchool of Chemical Engineering and Technology, Sun Yat-sen University, Zhuhai, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Shenzhen, ChinaLevi Regenerative Medicine Technologies, Zhuhai, ChinaLevi Regenerative Medicine Technologies, Zhuhai, ChinaHuamin Medicine Co Ltd., Zhuhai, ChinaHuamin Medicine Co Ltd., Zhuhai, ChinaGuangzhou Hongxiang Biological Medicine Technology Co Ltd., Guangzhou, ChinaWolfson Institute for Biomedical Research, University College London, London, UKHuamin Medicine Co Ltd., Zhuhai, ChinaThis new decade has started with a global pandemic of COVID-19 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), precipitating a worldwide health crisis and economic downturn. Scientists and clinicians have been racing against time to find therapies for COVID-19. Repurposing approved drugs, developing vaccines and employing passive immunization are three major therapeutic approaches to fighting COVID-19. Chicken immunoglobulin Y (IgY) has the potential to be used as neutralizing antibody against respiratory infections, and its advantages include high avidity, low risk of adverse immune responses, and easy local delivery by intranasal administration. In this study, we raised antibody against the spike (S) protein of SARS-CoV-2 in chickens and extracted IgY (called IgY-S) from egg yolk. IgY-S exhibited high immunoreactivity against SARS-CoV-2 S, and by epitope mapping, we found five linear epitopes of IgY-S in SARS-CoV-2 S, two of which are cross-reactive with SARS-CoV S. Notably, epitope SIIAYTMSL, one of the identified epitopes, partially overlaps the S1/S2 cleavage region in SARS-CoV-2 S and is located on the surface of S trimer in 3D structure, close to the S1/S2 cleavage site. Thus, antibody binding at this location could physically block the access of proteolytic enzymes to S1/S2 cleavage site and thereby impede S1/S2 proteolytic cleavage, which is crucial to subsequent virus-cell membrane fusion and viral cell entry. Therefore, the feasibility of using IgY-S or epitope SIIAYTMS-specific IgY as neutralizing antibody for preventing or treating SARS-CoV-2 infection is worth exploring.http://dx.doi.org/10.1155/2020/9465398 |
| spellingShingle | Yan Lu Yajun Wang Zhen Zhang Jingliang Huang Meicun Yao Guobin Huang Yuanyuan Ge Peichun Zhang Huaxin Huang Yong Wang Huiliang Li Wen Wang Generation of Chicken IgY against SARS-COV-2 Spike Protein and Epitope Mapping Journal of Immunology Research |
| title | Generation of Chicken IgY against SARS-COV-2 Spike Protein and Epitope Mapping |
| title_full | Generation of Chicken IgY against SARS-COV-2 Spike Protein and Epitope Mapping |
| title_fullStr | Generation of Chicken IgY against SARS-COV-2 Spike Protein and Epitope Mapping |
| title_full_unstemmed | Generation of Chicken IgY against SARS-COV-2 Spike Protein and Epitope Mapping |
| title_short | Generation of Chicken IgY against SARS-COV-2 Spike Protein and Epitope Mapping |
| title_sort | generation of chicken igy against sars cov 2 spike protein and epitope mapping |
| url | http://dx.doi.org/10.1155/2020/9465398 |
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